Welcome to Our Laboratory of Tokyo University of Agriculture and Technology

Our research activities:

1. Deciphering the protein folding mechanism of cytosolic molecular chaperones.
Predoldin - CCT (Chaperonin Containing TCP-1) System
Small heat shock proteins

Yamamoto YY, Yohda M, et al.
Asymmetry in the function and dynamics of the cytosolic group II chaperonin CCT/TRiC.
PLoS One. (2017) 12:e0176054. doi: 10.1371/journal.pone 0176054.

Zako T, Yohda M, et al.
Contribution of the C-Terminal Region of a Group II Chaperonin to its Interaction with Prefoldin and Substrate Transfer.
J Mol Biol. (2016) 428:2405-17. doi: 10.1016/j.jmb.2016.04.006.

Hanazono Y, Yohda M, et al.
Nonequivalence observed for the 16-meric structure of a small heat shock protein, SpHsp16.0, from Schizosaccharomyces pombe.
Structure. (2013) 21:220-8. doi: 10.1016/j.str.2012.11.015.

2. Application of bacterial nano-compartment, encapsulin by Dr. Noguchi

Tamura A, Noguchi K, et al.
Packaging guest proteins into the encapsulin nanocompartment from Rhodococcus erythropolis N771.
Biotechnol Bioeng. (2015) 112:13-20. doi: 10.1002/bit.25322.

3. Study on folding and assembly of antibody in CHO cell

4. Bioremediation of chlorinated compounds
Novel Dehaococcoides mccartyi strains
Structure and mechanism of reductive dehalogenases

Yohda M, et al.
Isolation and genomic characterization of a Dehalococcoides strain suggests genomic rearrangement during culture.
Sci Rep. (2017) 7:2230. doi: 10.1038/s41598-017-02381-0.

5. Development of olfactory sensing system using odorant receptors by Dr. Fukutani

Fukutani Y, et al.
Split luciferase complementation assay for the analysis of G protein-coupled receptor ligand response in Saccharomyces cerevisiae.
Biotechnol Bioeng. (2017) 114:1354-1361. doi: 10.1002/bit.26255.

Asakawa M, et al.
Modification of the response of olfactory receptors to acetophenone by CYP1a2.
Sci Rep. (2017) 7:10167. doi: 10.1038/s41598-017-10862-5.

6. Deciphering the role of cilia in mice by Dr. Shinohara

Shinohara K, et al.
Absence of Radial Spokes in Mouse Node Cilia Is Required for Rotational Movement but Confers Ultrastructural Instability as a Trade-Off.
Dev Cell. (2015) 35:236-46. doi: 10.1016/j.devcel.2015.10.001.

7. Structure and mechanism of Plasmodium translocon of exported proteins (PTEX)

Hakamada K, Yohda M, et al.
Expression and characterization of the Plasmodium translocon of the exported proteins component EXP2.
Biochem Biophys Res Commun. (2017) 482:700-705. doi: 10.1016/j.bbrc.2016.11.097.