Achievements
A paper by Dr. Tsukakoshi (C01 representative) has been published in Sensors & Diagnostics.
Dr. Tsukakoshi (C01 representative)'s paper has been published in Sensors & Diagnostics. This paper describes the difference in the reaction rate of LAMP, a DNA amplification technique, for DNA oligonucleotides containing methylated cytosine, and the development of a principle for measuring methylated cytosine, an epigenetic modification, based on the difference in the amount of LAMP reaction product.
Mizuki Tomizawa, Kiwako Watanabe, Kaori Tsukakoshi and Kazunori Ikebukuro. Detection of CpG methylation based on the change in amplification efficiency of strand-displacement DNA polymerase by CpG methylation. Sens. Diagn., 2025, Accepted Manuscript
A paper with Dr. Tsukakoshi (C01 representative) as co-first author has been published in Nucleic Acid Research.
A paper co-first authored by Dr. Tsukakoshi (C01 representative) has been published in Nucleic Acid Research. This paper describes the development of Bright Baby Spinach, a novel highly fluorescent and pH-responsive fluorescent aptamer, by fusing a fluorescent RNA aptamer with an RNA triplex structure that changes structure in response to pH. Dr. Tsukakoshi contributed to the characterization of the aptamer.
Kinuko Ueno†, Kaori Tsukakoshi†, Norito Takeuch, Shintaro Inaba, Andrea Idili, Alessandro Porchetta, Francesco Ricci, Kazunori Ikebukuro. Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion. Nucleic Acid Research Accepted (†These authors are equally contributed.)
A paper by Dr. Hashimoto (B01 representative) has been published in Nature Neuroscience.
A paper by Hashimoto (B01 representative) has been published in Nature Neuroscience. This is a paper about a new tauopathy model mouse created by the knock-in technique.
Watamura N, Foiani MS, Bez S, Bourdenx M, Santambrogio A, Frodsham C, Camporesi E, Brinkmalm G, Zetterberg H, Patel A, Kamano N, Takahashi M, Rueda-Carrasco J, Katsouri L, Fowler S, Turkes E, Hashimoto S, Sasaguri H, Saito T, Islam AS, Benner S, Endo T, Kobayashi K, Ishida C, Vendruscolo M, Yamada M, Duff KE, Saido TC “In vivo hyperphosphorylation of tau is associated with synaptic loss and behavioral abnormalities in the absence of tau seeds.” Nature Neuroscience doi: 10.1038/s41593-024-01829-7, 2024
A paper by Dr. Hashimoto (B01 representative) has been published in eNeuro.
A paper by Hashimoto (B01 representative) has been published in eNeuro. The paper is about characterization of microglia associated with tau pathology.
Nagata K, Hashimoto S, Joho D, Fujioka R, Matsuba Y, Sekiguchi M, Mihira N, Motooka D, Liu YC, Okuzaki D, Kikuchi M, Murayama S, Saido TC, Kiyama H, Sasaguri H “Tau accumulation induces microglial state alterations in Alzheimer’s disease model mice” eNeuro 26 ENEURO.0260-24, 2024
Dr. Hashimoto's (B01 Representative) paper was published in Life Science Alliance.
Dr. Hashimoto's (B01 Representative) paper has been published in Life Science Alliance. It has been revealed that 3pEAβ1-42, an Aβ species important in the formation of amyloid plaques, is promoted by the decrease in neprilysin, an Aβ metabolic enzyme.
Iwata N, Tsubuki S, Sekiguchi M, Watanabe-Iwata K. Matsuba Y, Kamano N, Fujioka R, Takamura R, Watamura N, Kakiya N, Mihira N, Morito T, Shirotani K, MA Mann D, Robinson CA, Hashimoto S, Sasaguri H, Saito T, Higuchi M, Saido TC. Metabolic resistance of Aβ3pE-42, a target epitope of the anti-Alzheimer therapeutic antibody, donanemab. Life Science Alliance 7(12) e202402650.
https://www.life-science-alliance.org/content/7/12/e202402650
Dr. Tsukakoshi's (C01 Representative) paper was published in Advanced Science.
Dr. Tsukakoshi's (C01 Representative) paper has been published in Advanced Science. We have developed a new detection system that combines enzyme-modified antibodies and DNAzyme-modified aptamers, and uses a continuous enzyme reaction that occurs only when each is simultaneously bound to a virus particle. Tsukakoshi has contributed to the development of the aptamer.
Miura, D., Hayashi, W, Hirano, K., Sasaki, I., Tsukakoshi, K., Kakizoe, H., Asai, S, Vavricka, C.J., Takemae, H., Mizutani, T., Tsugawa, W., Sode, K., Ikebukuro, K., and Asano, R. Proximity-Unlocked Luminescence by Sequential Enzymatic Reactions from Antibody and Antibody/Aptamer (PULSERAA): A Platform for Detection and Visualization of Virus-Containing Spots. (2024) Adv Sci (Weinh). e2403871.
Dr. Hashimoto's (B01 Representative) paper was published in FEBS Letters.
Dr. Hashimoto's (B01 Representative) paper was published in FEBS Letters.
We found that oxidative stress due to reduced glutathione levels leads to increased Aβ42 and promoted tau pathology.
Hashim KN, Matsuba Y, Takahashi M, Kamano N, Tooyama I, Saido TC, Hashimoto S*. Neuronal glutathione depletion elevates the Aβ42/Aβ40 ratio and tau aggregation in Alzheimer's disease mice. FEBS letters 598(13) 1576-1590.
Dr. Tsukakoshi's (C01 Representative) paper was published in Talanta.
Dr. Tsukakoshi's (C01 Representative) paper was published in Talanta.
This is research on the development of a FRET-type sensing system using an aptamer that specifically binds to the antibody drug bevacizumab.
Yamada T, Tsukakoshi K, Furusho A, Sugiyama E, Mizuno H, Hayashi H, Yamano T, Kumobayashi H, Hasebe T, Ikebukuro K, Toyo'oka T, Todoroki K. Simple and fast one-step FRET assay of therapeutic mAb bevacizumab using anti-idiotype DNA aptamer for process analytical technology. Talanta. 2024 Sep 1;277:126349.
https://www.sciencedirect.com/science/article/pii/S0039914024007288?via%3Dihub