2024年

1. The 2.5th generation enzymatic sensors based on the construction of quasi-direct electron transfer type NAD(P)-Dependent dehydrogenases
   Kurea Ikegai^†, Junko Okuda-Shimazaki^†, Truc Thanh Tran, Mika Hatada, Ryutaro Asano, Kazunori Ikebukuro, Wakako Tsugawa^* and Koji Sode^*
   Biosensors and Bioelectronics (2024) 255, 116219; doi.org/10.1016/j.bios.2024.116219, †: Contributed equally


2. Review:
   Structure of cytotoxic amyloid oligomers generated during disaggregation
   Toshisuke Kaku, Kazunori Ikebukuro and Kaori Tsukakoshi^*
   The Journal of Biochemistry (2024) in press; doi.org/10.1093/jb/mvae023


3. Rapid and Convenient Single-Chain Variable Fragment-Employed Electrochemical C-Reactive Protein Detection System
   Daimei Miura^†, Saki Motohashi^†, Ayaka Goto, Hayato Kimura, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro and Ryutaro Asano^*
   International Journal of Molecular Sciences (2024) 25, 5, 2859; doi.org/10.3390/ijms25052859, †: Contributed equally


4. Aptamer-enhanced particle aggregation inhibition assay for simple homogeneous protein detection using DNA aptamer and thermo-responsive magnetic nanoparticles
   Shunsuke Rokutani^†, Kentaro Hiraka^†, Hiroshi Saitoh^†, Taiki Saito, Yoshihiko Nonaka, Kinuko Ueno, Kaori Tsukakoshi, Noriyuki Ohnishi^* and Kazunori Ikebukuro^*
   Biosensors and Bioelectronics (2024) 245, 115827; doi.org/10.1016/j.bios.2023.115827, †: Contributed equally


5. Exploration and Application of DNA-Binding Proteins to Make a Versatile DNA–Protein Covalent-Linking Patch (D-Pclip): The Case of a Biosensing Element
   Erika Komiya, Shouhei Takamatsu, Daimei Miura, Kaori Tsukakoshi, Wakako Tsugawa, Koji Sode, Kazunori Ikebukuro and Ryutaro Asano^*
   Journal of the American Chemical Society (2024) 146, 6, 4087-4097; doi.org/10.1021/jacs.3c12668



2023年

1. Real-time monitoring of the amyloid β1–42 monomer-to-oligomer channel transition using a lipid bilayer system
   Yuri Numaguchi^†, Kaori Tsukakoshi^†, Nanami Takeuchi, Yuki Suzuki, Kazunori Ikebukuro and Ryuji Kawano^*
   PNAS Nexus (2023) 3, 1, 437; doi.org/10.1093/pnasnexus/pgad437, †: Contributed equally


2. Development of a liquid chromatography-based versatile bioanalysis for bevacizumab based on pretreatment combining aptamer affinity purification and centrifugal ultrafiltration concentration
   Kenichiro Todoroki, Daichi Hamada, Tomohiro Yamada, Taro Saito, Yutaka Shimizu, Eiji Sugiyama, Hajime Mizuno, Hideki Hayashi, Kaori Tsukakoshi and Kazunori Ikebukuro^*
   Analytical sciences (2023) 39, 37660341, 1805-1811; doi.org/10.1007/s44211-023-00417-2


3. Cancer therapeutic trispecific antibodies recruiting both T and natural killer cells to cancer cells
   Kouki Kimura, Atsushi Kuwahara, Saori Suzuki, Takeshi Nakanishi, Izumi Kumagai and Ryutaro Asano^*
   Oncology reports (2023) 50, 6, 1-10; doi.org/10.3892/or.2023.8649


4. Review:
   Protein engineering of antibody fragments for pharmaceutical production
   Atsushi Kuwahara, Kazunori Ikebukuro and Ryutaro Asano^*
   Applied physics reviews (2023) 10, 031311; doi.org/10.1063/5.0158032


5. Construction of IgG-Fab2 bispecific antibody via intein-mediated protein trans-splicing reaction
   Risa Yamada, Ishin Nakahara, Izumi Kumagai, Ryutaro Asano, Takeshi Nakanishi and Koki Makabe^*
   Scientific reports (2023) 13, 15961; doi.org/10.1038/s41598-023-43110-0


6. Functional integration of protein A binding ability to antibody fragments for convenient and tag-free purification
   Atsushi Kuwahara, Misae Nazuka, Yuri Kuroki, Kohei Ito, Shunsuke Watanabe, Izumi Kumagai and Ryutaro Asano^*
   Bioengineered (2023) 14 (1), 2259093; doi.org/10.1080/21655979.2023.2259093


7. Incorporation of a repeated polypeptide sequence in therapeutic antibodies as a universal masking procedure: A case study of T cell-engaging bispecific antibodies
   Atsushi Maejima, Saori Suzuki, Koki Makabe, Izumi Kumagai and Ryutaro Asano^*
   New Biotechnology (2023) 77 (25), 80-89; doi.org/10.1016/j.nbt.2023.07.004


8. Regulation of thrombin activity by ligand-induced topological alteration in a thrombin-binding aptamer
   Shogo Sasaki, Yue Ma, Takatsugu Hirokawa, Kazunori Ikebukuro, Masayuki Tera^* and Kazuo Nagasawa^*
   Chemical Communications (2023) 59 (57), 8862-8865; doi.org/10.1039/d3cc02308g


9. Development of interdigitated electrode based enzyme sensor for triglyceride monitoring using engineered oxygen insensitive glycerol 3-phosphate oxidase
   Inyoung Lee^†, Sheng Tong^†, Mika Hatada^†, Adrianna Masterson, Kentaro Hiraka, Junko Okuda-Shimazaki, Wakako Tsugawa, Gary Hughes and Koji Sode^*
   Sensors and Actuators Reports (2023) 5, 100164; doi.org/10.1016/j.snr.2023.100164, †: Contributed equally


10. Development of an Anti-Idiotype Aptamer-Based Electrochemical Sensor for a Humanized Therapeutic Antibody Monitoring
    Madoka Nagata, Jinhee Lee, Taro Saito, Kazunori Ikebukuro and Koji Sode^*
    International Journal of Molecular Sciences (2023) 24 (6), 5277; doi.org/10.3390/ijms24065277


11. Universal Design of Luciferase Fusion Proteins for Epigenetic Modifications Detection Based on Bioluminescence Resonance Energy Transfer
    Takamichi Miyata, Hazuki Shimamura, Ryutaro Asano and Wataru Yoshida^*
    Analytical Chemistry (2023) 95 (7), 3799-3805; 10.1021/acs.analchem.2c05066


12. Development of a Versatile Method to Construct Direct Electron Transfer-Type Enzyme Complexes Employing SpyCatcher/SpyTag System
    Takumi Yanase^†, Junko Okuda-Shimazaki^†, Ryutaro Asano, Koji Sode^* and Wakako Tsugawa^*
    International Journal of Molecular Sciences (2023) 24 (3), 1837; doi.org/10.3390/ijms24031837, †: Contributed equally


13. Identification of novel amyloidosis in dogs: α-S1-casein acquires amyloidogenicity in mammary tumor by overexpression and N-terminal truncation
    Tomoaki Murakami, Toshisuke Kaku, Kaori Tsukakoshi, Susumu Iwaide, Yoshiyuki Itoh, Miki Hisada, Kohji Nomura, Rikako Kubo, Kazunori Ikebukuro, Yukiko Sassa-O'Brien and Fuyuki Kametani^*
    Veterinary Pathology (2023) 60 (2), 203-213; doi.org/10.1177/03009858221148511


14. Review:
    Biosensors: Immunosensors
    Daimei Miura and Ryutaro Asano^*
    Encyclopedia of Sensors and Biosensors (2023) 298-314; doi.org/10.1016/B978-0-12-822548-6.00008-X


15. Review:
    タンパク質工学を駆使した多重特異性抗体の開発
    前島 敦、浅野 竜太郎^*
    生物工学会誌 (2023) 101 (6), 276



2022年

16. Microgravity environment grown crystal structure information based engineering of direct electron transfer type glucose dehydrogenase
    Junko Okuda-Shimazaki, Hiromi Yoshida, Inyoung Lee, Katsuhiro Kojima, Nanoha Suzuki, Wakako Tsugawa, Mitsugu Yamada, Koji Inaka, Hiroaki Tanaka and Koji Sode ^*
    Communication Biology (2022) 5, 1334; doi.org/10.1038/s42003-022-04286-9


17. Development of Alkaline Phosphatase-Fused Mouse Prion Protein and Its Application in Toxic Aβ Oligomer Detection
    Kaori Tsukakoshi^†*, Rikako Kubo^† and Kazunori Ikebukuro^*
    International Journal of Molecular Sciences (2022) 23 (23), 14588; doi.org/10.3390/ijms232314588


18. Enhancement of DNAzymatic activity using iterative in silico maturation
    Renzo A. Fenati, Zifei Chen, Yasuko Yamagishi, Kaori Tsukakoshi, Kazunori Ikebukuro, Anjay Manian, Salvy P. Russo, Tomohiko Yamazaki^* and Amanda V. Ellis^*
    Journal of Materials Chemistry B (2022) 10, 8960-8969; doi.org/10.1039/D2TB01638A


19. The state of water molecules induces changes in the topologies and interactions of G-quadruplex DNA aptamers in hydrated ionic liquid
    Kyoko Fujita^*, Takuya Honda, Kaori Tsukakoshi, Hiroyuki Ohno and Kazunori Ikebukuro^*
    Journal of Molecular Liquids (2022) 366, 120175; doi.org/10.1016/j.molliq.2022.120175


20. Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product‐inhibition mechanism and oxygen gatekeeper
    Kentaro Hiraka, Hiromi Yoshida, Wakako Tsugawa, Ryutaro Asano, Jeffrey T. La Belle, Kazunori Ikebukuro and Koji Sode^*
    Protein Science (2022) 31 (10), e4434; doi.org/10.1002/pro.4434


21. Review:
    Strategies to simplify operation procedures for applying labeled antibody-based immunosensors to point-of-care testing
    Hayato Kimura and Ryutaro Asano^*
    Analytical Biochemistry (2022) 654, 114806; 10.1016/j.ab.2022.114806


22. Effects of G-Quadruplex Ligands on the Topology, Stability, and Immunostimulatory Properties of G-Quadruplex-Based CpG Oligodeoxynucleotides
    Anh Thi Tram Tu, Kazuaki Hoshi, Yue Ma, Taiji Oyama, Satoko Suzuki, Kaori Tsukakoshi, Kazuo Nagasawa, Kazunori Ikebukuro and Tomohiko Yamazaki^*
    ACS Chemical Biology (2022) 17 (7), 1703-1713; doi.org/10.1021/acschembio.1c00904


23. CpG Methylation Altered the Stability and Structure of the i-Motifs Located in the CpG Islands
    Daiki Oshikawa, Shintaro Inaba, Yudai Kitagawa, Kaori Tsukakoshi and Kazunori Ikebukuro^*
    International Journal of Molecular Sciences (2022) 23 (12), 6467; doi.org/10.3390/ijms23126467


24. Development of a Lateral Flow Assay for Bevacizumab Using an Anti-Idiotype DNA Aptamer as a Capture Molecule
    Kenichiro Todoroki^*, Ryota Isobe, Tomohiro Yamada, Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Eiji Sugiyama, Hajime Mizuno, Hideki Hayashi and Kazunori Ikebukuro
    Chromatography (2022) 43 (2), 73-77; doi.org/10.15583/jpchrom.2022.008


25. A Thiol-reactive Phenazine Ethosulfate - A Novel Redox Mediator for Quasi-direct Electron-transfer-type Sensors
    Maya Fitriana, Kentaro Hiraka, Kazunori Ikebukuro, Koji Sode and Wakako Tsugawa^*
    Sensors and Materials (2022) 34 (6), 2105-2124; doi.org/10.18494/SAM3898


26. Development of a DNA aptamer that binds to the complementarity-determining region of therapeutic monoclonal antibody and affinity improvement induced by pH-change for sensitive detection
    Taro Saito, Yutaka Shimizu, Kaori Tsukakoshi, Koichi Abe, Jinhee Lee, Kinuko Ueno, Ryutaro Asano, Brian V. Jones, Tomohiro Yamada, Tatsuki Nakano, Jiaxing Tong, Asami Hishiki, Kodai Hara, Hiroshi Hashimoto, Koji Sode, Toshimasa Toyo'oka and Kenichiro Todoroki^* and Kazunori Ikebukuro^*
    Biosensors and Bioelectronics (2022) 203, 114027; doi.org/10.1016/j.bios.2022.114027


27. In Vitro Continuous 3 Months Operation of Direct Electron Transfer Type Open Circuit Potential Based Glucose Sensor: Heralding the Next CGM Sensor
    Inyoung Lee, Tsugawa Wakako, Kazunori Ikebukuro and Koji Sode^*
    Journal of diabetes science and technology (2022) 16 (5) ; doi.org/10.1177/19322968221092449


28. Transient potentiometry based d-serine sensor using engineered d-amino acid oxidase showing quasi-direct electron transfer property
    Shohei Takamatsu, Inyoung Lee, Jinhee Lee, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro, Jeffrey E. Dick and Koji Sode^*
    Biosensors and Bioelectronics (2022) 200, 113927; doi.org/10.1016/j.bios.2021.113927


29. Development of a POCT type insulin sensor employing anti-insulin single chain variable fragment based on faradaic electrochemical impedance spectroscopy under single frequency measurement
    Mukund Khanwalker^†, Rinko Fujita^†, Jinhee Lee, Ellie Wilson, Kohei Ito, Ryutaro Asano, Kazunori Ikebukuro, Jeffrey LaBelle and Koji Sode^*
    Biosensors and Bioelectronics (2022) 200, 113901; doi.org/10.1016/j.bios.2021.113901,†: Contributed equally


30. Light-induced production of isobutanol and 3-methyl-1-butanol by metabolically engineered cyanobacteria
    Shunichi Kobayashi, Shota Atsumi, Kazunori Ikebukuro, Koji Sode and Ryutaro Asano^*
    Microbial Cell Factories (2022) 21, 7; doi.org/10.1186/s12934-021-01732-x


31. Stabilization of VEGF i-motif structure by CpG methylation
    Kosuke Kimura, Daiki Oshikawa, Kazunori Ikebukuro^* and Wataru Yoshida^*
    Biochemical and Biophysical Research Communications (2022) 594, 88-92; doi.org/10.1016/j.bbrc.2022.01.054


32. An Amine-Reactive Phenazine Ethosulfate (arPES)—A Novel Redox Probe for Electrochemical Aptamer-Based Sensor
    Madoka Nagata^†, Jinhee Lee^†, Stephen Henley, Kazunori Ikebukuro and Koji Sode^*
    Sensors (Basel) (2022) 22(5), 1760; doi.org/10.1016/j.bbrc.2022.01.054, †: Contributed equally


33. Review:
    Fabrication of Fragment Antibody-Enzyme Complex as a Sensing Element for Immunosensing
    Miho Oda and Ryutaro Asano^*
    International Journal of Molecular Sciences (2022) 23(3), 1335; doi.org/10.3390/ijms23031335


34. Review:
    低分子型二重特異性抗体の高機能化戦略
    浅野　竜太郎, 熊谷　泉
    バイオサイエンスとインダストリー (2022) 80 (1), 40-41


35. Review:
    電気化学イムノセンサーを指向した人工抗体の開発
    浅野　竜太郎
    電気化学 (2022) 90 (1), 69



2021年

36. Detection of CpG Methylation in G-Quadruplex Forming Sequences Using G-Quadruplex Ligands
    Hijiri Hasegawa, Ikkei Sasaki, Kaori Tsukakoshi, Yue Ma, Kazuo Nagasawa, Shunsuke Numata, Yuuki Inoue, Yeji Kim and Kazunori Ikebukuro^*
    International Journal of Molecular Sciences (2021) 22 (23), 13159; doi.org/10.3390/ijms222313159


37. Cytotoxic Aβ Protofilaments Are Generated in the Process of Aβ Fibril Disaggregation
    Toshisuke Kaku^†, Kaori Tsukakoshi^*† and Kazunori Ikebukuro^*
    International Journal of Molecular Sciences (2021) 22 (23), 12780; doi.org/10.3390/ijms222312780, †: Contributed equally


38. Review:
    T Cell Bispecific Antibodies: An Antibody-Based Delivery System for Inducing Antitumor Immunity
    Daisuke Kamakura, Ryutaro Asano and Masahiro Yasunaga^*
    Pharmaceuticals (2021) 14 (11), 1172; doi.org/10.3390/ph14111172


39. Enhancement of the Immunostimulatory Effect of Phosphodiester CpG Oligodeoxynucleotides by an Antiparallel Guanine-Quadruplex Structural Scaffold
    Fika Ayu Safitri, Anh Thi Tram Tu, Kazuaki Hoshi, Miwako Shobo, Dandan Zhao, Arief Budi Witarto, Sony Heru Sumarsono, Ernawati, Arifin Giri-Rachman, Kaori Tsukakoshi, Kazunori Ikebukuro and Tomohiko Yamazaki^*
    Biomolecules (2021) 11(11), 1617; doi.org/10.3390/biom11111617


40. Continuous electrochemical monitoring of L-glutamine using redox-probe-modified L-glutamine-binding protein based on intermittent pulse amperometry
    Shouhei Takamatsu, Jinhee Lee, Ryutaro Asano, Wakako Tsugawa, Kazunori Ikebukuro and Koji Sode^*
    Sensors and Actuators B: Chemical (2021) 346, 130554; doi.org/10.1016/j.snb.2021.130554


41. Rapid, convenient, and highly sensitive detection of human hemoglobin in serum using a high-affinity bivalent antibody–enzyme complex
    Daimei Miura, Hayato Kimura, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode and Ryutaro Asano^*
    Talanta (2021) 234, 122638; doi.org/10.1016/j.talanta.2021.122638


42. Artificial complementary chromatic acclimation gene expression system in Escherichia coli
    Dwi Ariyanti, Kazunori Ikebukuro^* and Koji Sode^*
    Microbial Cell Factories (2021) 20, 128; doi.org/10.1186/s12934-021-01621-3


43. G-quadruplex-forming aptamer enhances the peroxidase activity of myoglobin against luminol
    Kaori Tsukakoshi^†, Yasuko Yamagishi^†, Mana Kanazashi, Kenta Nakama, Daiki Oshikawa, Nasa Savory, Akimasa Matsugami, Fumiaki Hayashi, Jinhee Lee, Taiki Saito, Koji Sode, Kanjana Khunathai, Hitoshi Kuno and Kazunori Ikebukuro^*
    Nucleic Acids Research (2021) 49 (11), 6069-6081; doi.org/10.1093/nar/gkab388, †: Contributed equally


44. Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins
    Jinhee Lee, Kentaro Teramoto, Tomomi Yokoyama, Kinuko Ueno, Kaori Tsukakoshi, Koji Sode and Kazunori Ikebukuro^*
    Data in Brief (2021) 36, 107028; doi.org/10.1016/j.dib.2021.107028


45. Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement
    Mika Hatada^†, Noya Loew^†, Junko Okuda-Shimazaki, Mukund Khanwalker, Wakako Tsugawa, Ashok Mulchandani and Koji Sode^*
    Molecules (2021) 26 (3), 734; doi.org/10.3390/molecules26030734, †: Contributed equally


46. Evaluation of intercellular cross-linking abilities correlated with cytotoxicities of bispecific antibodies with domain rearrangements using AFM force-sensing
    Atsushi Maejima, Kenta Ishibashi, Hyonchol Kim, Izumi Kumagai and Ryutaro Asano^*
    Biosensors and Bioelectronics (2021) 178, 113037; doi.org/10.1016/j.bios.2021.113037


47. Review:
    G-quadruplex: Flexible conformational changes by cations, pH, crowding and its applications to biosensing
    Maui Nishio, Kaoti Tsukakoshi and Kazunori Ikebukuro^*
    Biosensors and Bioelectronics (2021) 178, 113030; doi.org/10.1016/j.bios.2021.113030


48. Development of glycated peptide enzyme sensor based flow injection analysis system for haemoglobin A1c monitoring using quasi-direct electron transfer type engineered fructosyl peptide oxidase
    Mika Hatada, Satomi Saito, Satoshi Yonehara, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro and Koji Sode^*
    Biosensors and Bioelectronics (2021) 177, 112984; doi.org/10.1016/j.bios.2021.112984


49. Rational design of direct electron transfer type L-lactate dehydrogenase for the development of multiplexed biosensor
    Kentaro Hiraka, Wakako Tsugawa, Ryutaro Asano, Murat A. Yokus, Kazunori Ikebukuro, Michael A. Daniele and Koji Sode^*
    Biosensors and Bioelectronics (2021) 176, 112933; doi.org/10.1016/j.bios.2020.112933


50. Strategic design and improvement of the internal electron transfer of heme b domain-fused glucose dehydrogenase for use in direct electron transfer-type glucose sensors
    Kohei Ito, Junko Okuda-Shimazaki, Katsuhiro Kojima, Kazushige Mori, Wakako Tsugawa, Ryutaro Asano, Kazunori Ikebukuro and Koji Sode^*
    Biosensors and Bioelectronics (2021) 176, 112911; doi.org/10.1016/j.bios.2020.112911


51. A self-powered glucose sensor based on BioCapacitor principle with micro-sized enzyme anode employing direct electron transfer type FADGDH
    Inyoung Lee, Junko Okuda-Shimazaki, Wakako Tsugawa, Kazunori Ikebukuro and Koji Sode^*
    Journal of Physics-Energy (2021) 3, 034009; doi.org/10.1088/2515-7655/abee32


52. Anti-EGFR antibody 528 binds to domain III of EGFR at a site shifted from the cetuximab epitope
    Koki Makabe, Takeshi Yokoyama, Shiro Uehara, Tomomi Uchikubo-Kamo, Mikako Shirouzu, Kouki Kimura, Kouhei Tsumoto, Ryutaro Asano, Yoshikazu Tanaka and Izumi Kumagai^*
    Scientific reports (2021) 11 (1), 5790; doi.org/10.1038/s41598-021-84171-3


53. Rapid and homogeneous electrochemical detection by fabricating a high affinity bispecific antibody-enzyme complex using two Catcher/Tag systems
    Hayato Kimura, Daimei Miura, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode and Ryutaro Asano^*
    Biosensors and Bioelectronics (2021) 175, 112885; doi.org/10.1016/j.bios.2020.112885


54. Mechanism of action of a T cell-dependent bispecific antibody as a breakthrough immunotherapy against refractory colorectal cancer with an oncogenic mutation
    Daisuke Kamakura, Ryutaro Asano, Hiroki Kawai and Masahiro Yasunaga^*
    Cancer Immunology, Immunotherapy (2021) 70, 177-188; doi.org/10.1007/s00262-020-02667-9


55. Review:
    二重特異性がん治療抗体の機能的な構造の理解に向けたあゆみ
    浅野　竜太郎, 真壁　幸樹, 田中　良和, 熊谷　泉
    医学のあゆみ (2021) 278 (6), 617-622


56. Review:
    POCTを志向した均一かつ汎用的な抗体酵素複合体の調製法の開発と電気化学イムノセンサへの展開
    三浦　大明, 浅野　竜太郎
    日本応用酵素協会誌 (2021) 56, 1-9



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