2026年

2. Competitive-SELEX discovery of DNA aptamers selective for neurofilament light chain in human plasma
   Miyu Matsumoto, Kazunori Ikebukuro^*, Kaori Tsukakoshi
   Biochemical and Biophysical Research Communications (2026) 796, 153151; doi.org/10.1016/j.bbrc.2025.153151



2025年

2. Turning on Protein Function Inhibited by DNA Aptamers Employing a Covalent DNA-Binding Protein
   Takeru Kanazu, Erika Komiya, Daimei Miura, Kaori Tsukakoshi, Kazunori Ikebukuro^*,Tomohiko Yamazaki^*, and Ryutaro Asano^*
   ACS Nanoscience Au (2025) in press; doi.org/10.1021/acsnanoscienceau.5c00143


3. Detection of Structural Changes in G-Quadruplex-Forming DNA Oligonucleotides via DNA Methylation Based on Luminol Chemiluminescence Catalyzed by Myoglobin
   Shintaro Inaba, Haruka Kawai, Mizuki Tomizawa, Daimei Miura, Kaori Tsukakoshi and Kazunori Ikebukuro^*
   Biosensors (2025) 16, 1, 1; doi.org/10.3390/bios16010001


4. Transient analysis and engineering of bacterial metabolic pathways using cell-penetrating peptide-peptide nucleic acid conjugates
   Yugo Kawabuchi, Shunichi Kobayashi, Jaeyoung Yu, Gota Kasai, Miku Kobe, Rei Suo, Shiro Itoi, Koji Sode, Takahiro Muraoka, Ryutaro Asano and Tetsushi Mori^*
   Microbial Cell Factories (2025) in press; doi.org/10.1186/s12934-025-02909-4


5. Low-Cost Polyphenol-Polypyrrole Molecularly Imprinted Sensor for Point-of-Care Alzheimer's Detection
   Ajith Mohan Arjun^†, Sudhaunsh Deshpande^†, Guoyi Liu, Daimei Miura, Krzysztof Pawlak,Tokuda Takahiko, Makoto Higuchi, Miyu Matsumoto, Tomoko Umemura, Kaori Tsukakoshi,and Sanjiv Sharma^*
   ACS Sensors (2025) 10, 11, 8435-8446; doi.org/10.1021/acssensors.5c01816, †: Contributed equally


6. Construction and Characterization of a Novel Direct Electron Transfer Type Enzymatic Sensor Using Spermidine Dehydrogenase
   Sheng Tong^†, Yuki Yaegashi^†, Mao Fukushi^†, Takumi Yanase, Junko Okuda-Shimazaki, Ryutaro Asano, Kazunori Ikebukuro, Madoka Nagata, Koji Sode^* and Wakako Tsugawa^*
   Biosensors (2025) 15, 10, 681; doi.org/10.3390/bio15100681, †: Contributed equally


7. Bispecific antibody-antigen complex structures reveal activity enhancement by domain rearrangement
   Kyohei Sato, Shiro Uehara, Atsushi Tsugita, Mayuka Ishii, Shieru Ishiyama, Atsushi Maejima, Ishin Nakahara, Misae Nazuka, Takashi Matsui, Gatsogiannis Christos, Takeshi Yokoyama, Izumi Kumagai, Koki Makabe^*, Ryutaro Asano^* and Yoshikazu Tanaka^*
   Cell Reports (2025) 44, 115965; doi.org/10.1016/j.celrep.2025.115965


8. Targeting G-Quadruplex with Bis-thiourea Compounds Inhibits SARS-CoV-2 Replication
   Shogo Sasaki, Shogo Nakajima, Rena Nohara, Hiroyuki Endo, Norito Takeuchi, Taiji Oyama, Naoya Iwano, Kaori Tsukakoshi, Kazunori Ikebukuro, Akira Shiraishi, Kazuo Nagasawa, Koichi Watashi^* and Masayuki Tera^*
   ACS Infectious Diseases (2025) 8, 11; doi.org/10.1021/acsinfecdis.5c00095


9. Engineered single chain variable fragments (scFvs) with improved pH-dependent kinetics for use in continuous biosensor applications
   Ellie Wilson, David Probst, Mai Hamasaki, Miho Oda, Varun Kochar, Qianming Xu, Ayumi Tanaka, Hirobumi Suzuki, Ryutaro Asano and Koji Sode^*
   RSC Advances (2025) 15, 25337; doi.org/10.1039/D5RA02051D


10. Evaluation of the effects of G4 ligands on the interaction between G-quadruplexes and their binding proteins
    Yuma Tanaya, Manaho Sashida, Hisao Masai, Hiroyuki Sasanuma, Daimei Miura, Ryutaro Asano, Kazuo Nagasawa and Masayuki Tera^*
    Chemical Communications (2025) 61, 11790; doi.org/10.1039/D5CC02801A


11. Functional and Structural Analyses of Diverse G-Quadruplex and Non-G-Quadruplex Structures Formed by Guanine-Rich Nucleic Acids: A Study on the Insulin Aptamer
    Naoya Iwano, Taiji Oyama, Mizuki Tomizawa, Shintaro Inaba, Ryuma Mori, Yasumoto Nakazawa, Koji Sode, Kaori Tsukakoshi, Takayuki Nakano and Kazunori Ikebukuro^*
    Small (2025) 2501336; doi.org/10.1002/smll.202501336


12. Immunological synapse formation as a key mechanism in T cell-dependent bispecific antibody-mediated immune activation and cytotoxicity
    Rikuto Nakamura, Ryo Tsumura, Takahiro Anzai, Ryutaro Asano and Masahiro Yasunaga^*
    Cancer Immunology, Immunotherapy (2025) 75, 246; doi.org/10.1007/s00262-025-04036-w


13. Bright and pH-sensitive Baby Spinach aptamer with RNA triplex fusion
    Kinuko Ueno^†, Kaori Tsukakoshi^†, Norito Takeuchi, Shintaro Inaba, Andrea Idili, Alessandro Porchetta, Francesco Ricci^* and Kazunori Ikebukuro^*
    Nucleic Acids Research (2025) 53, gkaf151; doi.org//10.1093/nar/gkaf151, †: Contributed equally


14. Design of a prodrug bispecific antibody masked by a functional molecule for lymphocyte activation for cancer therapy
    Daimei Miura^†, Yuki Kato^†, Masahiro Yasunaga, Izumi Kumagai and Ryutaro Asano^*
    Journal of Biological Engineering (2025) 19, 45; doi.org/10.1186/s13036-025-00517-9, †: Contributed equally


15. Expressing and functionally evaluating anticancer bispecific antibody fragments using cyanobacterium Synechocystis sp. PCC6803 for sustainable production
    Gota Kasai, Sayumi Shioya, Miku Kobe, Shunichi Kobayashi, Shouhei Takamatsu, Koki Makabe, Izumi Kumagai, Koji Sode and Ryutaro Asano^*
    Biochemical Engineering Journal (2025) 221, 109776; doi.org/10.1016/j.bej.2025.109776


16. Evaluation of an anti-CD3 VHH and construction of an anti-CD3/anti-EGFR bispecific tandem VHH as a cancer cell targeting drug construct
    Takuya Asanuma, Peiwei Ding, Yuki Kato, Takeshi Nakanishi, Ryutaro Asano and Koki Makabe^*
    Biochemistry and Biophysics Reports (2025) 42, 102015; doi.org/10.1016/j.bbrep.2025.102015


17. Detection of CpG methylation based on the change in amplification efficiency of strand-displacement DNA polymerase by CpG methylation
    Mizuki Tomizawa, Kiwako Watanabe, Kaori Tsukakoshi and Kazunori Ikebukuro^*
    Sensors & Diagnostics (2025) 4, 397; doi.org/10.1039/D5SD00012B


18. Protocol for circular dichroism spectral analysis of the thermal stability of CpG-methylated quadruplex structures
    Momo Moriya^*†, Taiji Oyama^*†, Masanori Goto, Kazunori Ikebukuro and Wataru Yoshida^*
    STAR Protocols (2025) 6, 103646; doi.org/10.1016/j.xpro.2025.103646, †: Contributed equally


19. Immunostimulatory Effects of Guanine-Quadruplex Topologies as Scaffolds for CpG Oligodeoxynucleotides
    Soumitra Pathak, Nguyen Bui Thao Le, Taiji Oyama, Yusuke Odahara, Atsuya Momotake, Kazunori Ikebukuro, Chiho Kataoka-Hamai, Chiaki Yoshikawa, Kohsaku Kawakami, Yoshihisa Kaizuka and Tomohiko Yamazaki^*
    Biomolecules (2025) 15, 95; doi.org/10.3390/biom15010095


20. Development of a mertansine-specific DNA aptamer and novel high-throughput sandwich enzyme-linked oligonucleotide assay for quantification and characterization of trastuzumab emtansine
    Tomohiro Yamada, Aogu Furusho, Kenji Kojima, Eiji Sugiyama, Hajime Mizuno, Kaori Tsukakoshi, Hideki Hayashi, Takeshi Yamano, Takashi Hasebe, Toshimasa Toyo’oka, Kazunori Ikebukuro and Kenichiro Todoroki^*
    Biosensors and Bioelectronics (2025) 272, 117108; doi.org/10.1016/j.bios.2024.117108


21. Development of tetravalent antibody–enzyme complexes employing a lactate oxidase and the application to electrochemical immunosensors
    Miho Oda, Kentaro Hiraka, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode^* and Ryutaro Asano^*
    Biosensors and Bioelectronics (2025) 267, 116741; doi.org/10.1016/j.bios.2024.116741


22. A Versatile Method to Create Antibody/Split-Enzyme Complexes and Its Application to a Rapid, Homogeneous, and Universal Electrochemical Immunosensing System
    Yuka Tobita^†, Kensuke Hirano^†, Daimei Miura^†, Yuma Hatano, Wakako Tsugawa, Kazunori Ikebukuro, Koji Sode^* and Ryutaro Asano^*
    Advanced Sensor Research (2025) 4, 2400112; doi.org/10.1002/adsr.202400112, †: Contributed equally


23. Review:
    Challenges in realizing therapeutic antibody biosensing
    Madoka Nagata, Ellie Wilson, Kazunori Ikebukuro^* and Koji Sode^*
    Trends in Biotechnology (2025) in press; doi.org/10.1016/j.tibtech.2025.07.003


24. Review:
    がん細胞―T細胞間架橋を志向した二重特異性抗体の開発と高機能化
    浅野竜太郎, 三浦大明
    Drug Delivery System (2025) 40 (5), 350-359



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