The SCP tag is a central piece of πBIO. Vaccine seeds developed by πBIO differ from conventional vaccines in that it uses not the virus itself but a fragment of the viral protein. The protein is expressed in E. coli and purified to a high purity, and used as the vaccine antigen. Protein fragments expressed in E. coli are inorganic molecules, non-toxic, and have overwhelming advantages over conventional vaccines in terms of safety, price, and development speed. However, the immunogenicity of recombinant proteins expressed in E. coli most often weak, making it unfit to use them as vaccine antigens. Therefore, to date, there is no subunit vaccine using proteins expressed in E. coli as antigens. SCP tag is the central technology of πBIO. SCP tag dramatically improves the immunogenicity of proteins and induces the generation of antiserum with neutralizing ability. In addition to the SCP tag, πBIO also includes methods to identify and produce viral proteins without affecting their structure and function, as well as methods to rapidly generate monoclonal antibodies.
Focus on the Receptor Binding Domain(RBD) of their spike protein that allow viruses to bind to host cells. RBD is created by protein engineering. The addition of a SCP tag consisting of a few residues to RBDs enhances immunogenicity and induces immune memory. Anti-RBD serum is a prime vaccine seed candidate because of its neutralizing ability.
Central technologies of πBIO are the fragmentation viral proteins, the enhancement of their immunogenicity, and their usage in pharmaceuticals and other products. The advantages of using πBIO include the following.