Taishi Umezawa - Tokyo University of Agriculture and Technology - Keywords: Plant Molecular Biology, Arabidopsis, abiotic stress, drought tolerance, salt tolerance, abscisic acid, ABA, signal transduction, proteome, phosphoproteomics, protein phosphoryaltion
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Peer-reviewed papers

  1. Li, Y., Kamiyama, Y., Minegishi, F., Tamura, Y., Yamashita, K., Katagiri, S., Takase, H., Otani, M., Tojo, R., Rupp, G.E., Suzuki, T., Kawakami, N., Peck, S.C. and Umezawa, T. (2024) “Group C MAP kinases phosphorylate MBD10 to regulate ABA-induced leaf senescence in Arabidopsis.” Plant J. 118(6):1747-1759. [Pubmed] [Journal]

  2. Borjigin, T., Ohkama-Ohtsu, N., Agake, S., Ngo, N.P., Maeda, M., Yokoyama, T., Umezawa, T. and Yamada, T. (2024) “Effects of plant growth-promoting rhizobacteria Bacillus pumilus TUAT1 strain inoculation on Arabidopsis thaliana seedling growth.” Asian J. Plant Sci. 23: 35-45.

  3. Katagiri, S., Kamiyama, Y., Yamashita, K., Iizumi, S., Suzuki, R., Aoi, Y., Takahashi, F., Kasahara, H., Kinoshita, T. and Umezawa, T. (2024) “Accumulation of phosphorylated SnRK2-Substrate 1 promotes drought escape in Arabidopsis.” Plant Cell Physiol. 65(2):259-268. [Pubmed] [Journal]

  4. Kamiyama, Y., Hirotani, M., Ishikawa, S., Minegishi, F., Katagiri, S., Rogan, C.J., Takahashi, F., Nomoto, M., Ishikawa, K., Kodama, Y., Tada, Y., Takezawa, D., Anderson, J.C., Peck, S.C., Shinozaki, K. and Umezawa, T. (2021) “Arabidopsis group C Raf-like protein kinases negatively regulate abscisic acid signaling and are direct substrates of SnRK2.” Proc. Natl. Acad. Sci. USA. 118(30):e2100073118. [Pubmed] [Journal]

  5. Islam, M., Inoue, T., Hiraide, M., Khatun, N., Jahan, A., Kuwata, K., Katagiri, S., Umezawa, T., Yotsui, I., Sakata, Y. and Takezawa, D. (2021) “Activation of SnRK2 by Raf-like kinase ARK represents a primary mechanism of ABA and abiotic stress responses.” Plant Physiol. 185(2):533-546. [Pubmed] [Journal]

  6. Matsuoka, S., Sato, K., Maruki-Imamura, R., Noutoshi, Y., Okabe, T., Kojima, H. and Umezawa, T. (2021) “Identification of novel compounds that inhibit SnRK2 kinase activity by high-throughput screening.” Biochem. Biophys. Res. Commun. 537:57-63. [Pubmed] [Journal]

  7. Kamal, M.M., Ishikawa, S., Takahashi, F., Suzuki, K., Kamo, M., Umezawa, T., Shinozaki, K., Kawamura, Y. and Uemura, M. (2020) “Large-scale phosphoproteomic study of Arabidopsis membrane proteins reveals early signaling events in response to cold.” Int. J. Mol. Sci. 21(22):8631. [Pubmed] [Journal]

  8. Katsuta, S., Masuda, G., Bak, H., Shinozawa, A., Kamiyama, Y., Umezawa, T., Takezawa, D., Yotsui, I., Taji, T. and Sakata, Y. (2020) Arabidopsis Raf-like kinases act as positive regulators of subclass III SnRK2 in osmostress signaling. Plant J. 103(2): 634-644. [Pubmed] [Journal]

  9. Ishikawa, S., Barrero, J.M., Takahashi, F., Peck, S.C., Gubler, F., Shinozaki, K. and Umezawa, T. (2019) “Comparative phosphoproteomic analysis reveals a decay of ABA signaling in barley embryos during after-ripening process.” Plant Cell Physiol. 60: 2758-2768. [Pubmed] [Journal]

  10. Barrero, J.M., Dorr, M., Talbot, M.J., Ishikawa, S., Umezawa, T., Alonso-Peral, M.M., White, R.G. and Gubler, F. (2019) “A role for PM19-Like 1 in seed dormancy in Arabidopsis.” Seed Sci. Res. 29: 184-196. [Journal]

  11. Ishikawa, S., Barrero, J.M., Takahashi, F., Peck, S.C., Gubler, F., Shinozaki, K. and Umezawa, T. (2019) “Comparative phosphoproteomic analysis of barley embryos with different dormancy during imbibition.” Int. J. Mol. Sci. 20: 451. [Pubmed] [Journal]

  12. Shinozawa, A., Otake, R., Takezawa, D., Umezawa, T., Komatsu, K., Tanakam K., Amagai, A., Ishikawa, S., Hara, Y., Kamisugi, Y., Cuming, A.C., Hori, K., Ohta, H., Takahashi, F., Shinozaki, K., Hayashi, T., Taji, T. and Sakata, Y. (2019) “SnRK2 protein kinases represent an ancient system in plants for adaptation to a terrestrial environment.” Commun. Biol. 2: 30. [Pubmed] [Journal]

  13. Jahan, A., Komatsu, K., Wakida-Sekiya, M., Hiraide, M., Tanaka, K., Ohtake, R., Umezawa, T., Toriyama, T., Shinozawa, A., Yotsui, I., Sakata, Y. and Takezawa, D. (2019) “Archetypal roles of an abscisic acid receptor in drought and sugar responses in liverworts.” Plant Physiol. 179: 317-328. [Pubmed] [Journal]

  14. Osako, Y., Takata, N., Nuoendagula, N., Ishikawa, S., Umezawa, T., Taniguchi, T. and Kajita, S. (2019) “Expression analysis of cellulose synthases that comprise the Type II complex in hybrid aspen.” Plant Biol. 21(2):361-370. [Pubmed] [Journal]

  15. Amagai, A., Honda, Y., Ishikawa, S., Hara, Y., Kuwamura, M., Shinozawa, A., Sugiyama, N., Ishihama, Y., Takezawa, D., Sakata, Y., Shinozaki, K. and Umezawa, T. (2018) “Phosphoproteomic profiling reveals ABA-responsive phosphosignaling pathways in Physcomitrella patens.” Plant J. 94: 699-708. [Pubmed] [Journal]

  16. Yu, X., Ohtani, M., Kusano, M., Nishikubo, N., Uenoyama, M., Umezawa, T., Saito, K., Shinozaki, K. and Demura, T. (2017) “Enhancement of abiotic stress tolerance in poplar by overexpression of key Arabidopsis stress response genes, AtSRK2C and AtGolS2.” Mol. Breeding 37: 57. [Journal]

  17. Saruhashi, M., Kumar-Ghosh, T., Arai, K., Ishizaki, Y., Hagiwara, K., Komatsu, K., Shiwa, Y., Umezawa, T., Sakata, Y. and Takezawa, D. (2015) “Plant Raf-like kinase integrates abscisic acid and hyperosmotic stress signaling upstream of SNF1-related protein kinase2.” Proc. Nat. Acad. Sci. USA. 112(46): E6388-6396. [Pubmed] [Journal]

  18. Saruhashi, M., Kumar-Ghosh, T., Arai, K., Ishizaki, Y., Hagiwara, K., Komatsu, K., Shiwa, Y., Umezawa, T., Sakata, Y. and Takezawa, D. (2015) “Plant Raf-like kinase integrates abscisic acid and hyperosmotic stress signaling upstream of SNF1-related protein kinase2.” Proc. Nat. Acad. Sci. USA. 112(46): E6388-6396. [Pubmed] [Journal]

  19. Ito, T., Kondoh, Y., Yoshida, K., Umezawa, T., Shimizu, T., Shinozaki, K. and Osada, H. (2015) "Novel abscisic acid antagonists identified with chemical array screening." ChemBioChem 16(17): 2471-2478. [Pubmed] [Journal]

  20. Umezawa, T., Sugiyama, N., Takahashi, F., Anderson, J.C., Ishihama, Y., Peck, S.C. and Shinozaki, K. (2013) “Genetics and phosphoproteomics reveal a protein phosphorylation network in the abscisic acid pathway in Arabidopsis thaliana.” Sci. Signal. 6(270): rs8. [Pubmed] [Journal] [Free Full text] [Reprint]

  21. Osakabe, Y., Arinaga, N., Umezawa, T., Katsura, S., Nagamachi, K., Tanaka, H., Ohiraki, H., Yamada, K., Seo, S.-U., Abo, M., Yoshimura, E., Shinozaki, K., Yamaguchi-Shinozaki, K. (2013) “Osmotic stress response and plant growth controlled by the potassium transporters in Arabidopsis.” Plant Cell 25(2): 609-624. [Pubmed] [Journal]

  22. *Mizoguchi, M., *Umezawa, T., Nakashima, K., Kidokoro, S., Takasaki, H., Fujira, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2010) “Two closely related subclass II SnRK2 protein kinases cooperatively regulate drought-inducible gene expression.” Plant Cell Physiol. 51(5):842-847.  *equal contribution. [Pubmed] [Journal]

  23. Schmutz, J., Cannon, S.B., Schlueter, J., Ma, J., Hyten, D., Song, Q., Mitros, T., Nelson, W., May, G.D., Gill, N., Peto, M., Goodstein, D., Thelen, J.J., Cheng, J., Sakurai, T., Umezawa, T., Shinozaki, K., Du, J., Bhattacharyya, M., Sandhu, D., Grant, D., Joshi, T., Libault, M., Zhang, X-.C., Xu, D., Futrell-Griggs, M., Abernathy, B., Hellsten, U., Berry, K., Grimwood, J., Wing, R.A., Cregan, P., Stacey, G., Specht, J., Rokhsar, D., Shoemaker, R.C. and Jackson, S.A. (2010) “Genome sequence of the paleopolyploid soybean (Glycine max (L.) Merr.)” Nature 463(7278):178-183. [Pubmed] [Journal]

  24. Choi, P., Mano, Y., Ishikawa, A., Odashima, M., Umezawa, T., Fujimura, T., Takahata, Y. and Komatsuda, T. (2010) “Identification of QTLs controlling somatic embryogenesis using RI population of cultivar × weedy soybean.” Plant Biotech. Rep. 4(1): 23-27.

  25. Fujita, Y., Nakashima, K., Yoshida, T., Katagiri, T., Kidokoro, S., Kanamori, N., Umezawa, T., Fujita, M., Maruyama, K., Ishiyama, K., Kobayashi, M., Nakasone, S., Yamada, K., Ito, T., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2009) “Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis.” Plant Cell Physiol. 50(12):2123-2132.

  26. Sato, A.,  Sato, Y.,  Fukao, Y.,  Fujiwara, M.,  Umezawa, T., Shinozaki, K., Hibi, T.,  Taniguchi, M.,  Miyake, H.,  Goto, D.B. and Uozumi, N. (2009) “Threonine  at  position  306  of  the  KAT1  potassium  channel  is  essential  for channel activity and is a target site for ABA-activated SRK2E/OST1/SnRK2.6 protein kinase.” Biochem. J. 424(3):439-448.

  27. Umezawa, T., Sugiyama, Y., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T. and Shinozaki, K. (2009) “Type 2C protein phosphatases directly regulate abscisic acid-activated protein kinases in Arabidopsis.”  Proc. Nat. Acad. Sci. USA. 106(41): 17588-17593. Pubmed] [Free Full Text]

  28. Nakashima, K., Fujita, Y., Kanamori, N., Katagiri, T., Umezawa, T., Kidokoro, S., Maruyama, K., Yoshida, T., Ishiyama, K., Kobayashi, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2009) “Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3 involved in ABA-signaling are essential for the control of seed development and dormancy.” Plant Cell Physiol. 50(7):1345-1363.

  29. Myouga, F., Hosoda, C., Umezawa, T., Iizumi, H., Kuromori, T., Motohashi, R., Shono, Y., Nagata, N., Ikeuchi, M. and Shinozaki, K. (2008) “Heterocomplex of Arabidopsis chloroplast-targeted iron superoxide dismutases, FSD2 and FSD3, mediates defense against oxidative stress in chloroplast nucleoids and is essential for chloroplast development.” Plant Cell 20(11):3148-3162.

  30. Umezawa, T., Sakurai, T., Totoki, Y., Toyoda, A., Seki, M., Ishiwata, A., Akiyama, K., Kurotani, A., Yoshida, T., Mochida, K., Kasuga, M., Todaka, D., Maruyama, K., Nakashima, K., Enju, A., Mizukado, S., Ahmed, S., Yoshiwara, K., Harada, K., Tsubokura, Y., Hayashi, M., Sato, S., Anai, T., Ishimoto, M., Funatsuki, H., Teraishi, M., Osaki, M., Shinano, T., Akashi, R., Sakaki, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2008) “Sequencing and Analysis of Approximately 40,000 Soybean cDNA Clones from a Full-length Enriched cDNA Library.” DNA Res. 15(6):333-346. [Pubmed] [Journal]

  31. Yasuda, M., Ishikawa, A., Jikumaru, Y., Seki, M., Umezawa, T., Asami, T., Maruyama-Nakashita, A., Kudo, T., Shinozaki, K., Yoshida, S. and Nakashita, H. (2008) “Antagonistic Interaction between Systemic Acquired Resistance and the Abscisic Acid–Mediated Abiotic Stress Response in Arabidopsis.” Plant Cell 20(6):1678-1692.

  32. Qin, F., Sakuma Y., Tran, LP., Maruyama, K., Kidokoro, S., Fujita, Y., Fujita, M., Umezawa, T., Sawano, Y., Miyazono, K., Tanokura, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2008) “Arabidopsis DREB2A-Interacting Proteins Function as RING E3 Ligases and Negatively Regulate Plant Drought Stress–Responsive Gene Expression.” Plant Cell 20(6):1693-1707

  33. Akihiro, T., Umezawa, T., Ueki, C., Lobna, B. M., Mizuno, K. and Fujimura, T. (2006) “Genome wide cDNA-AFLP analysis of genes rapidly induced by combined sucrose and ABA treatment in rice cultured cells.” FEBS Lett. 580(25): 5947-5952.

  34. Umezawa, T., Okamoto, M., Kushiro, T., Nambara, E., Oono, Y., Seki, M., Kobayashi. M., Kamiya, Y. and Shinozaki, K. (2006) “CYP707A3, a major ABA 8”-hydroxylase involved in dehydration and rehydration process in Arabidopsis thaliana.” Plant J. 46: 171-182. [Pubmed] [Journal]

  35. Yoshida, R., Umezawa, T., Mizoguchi, T., Takahashi, S., Takahashi, F. and Shinozaki, K.(2006) “Regulatory domain of SRK2E/OST1 interacts with ABI1 and integrates ABA and osmotic stress signals controlling stomatal closure in Arabidopsis.” J. Biol. Chem. 281(8): 5310-5318.

  36. Furihata, T., Maruyama, K., Fujita, Y., Umezawa, T., Yoshida, R., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2006) “ABA-dependent multisite phosphorylation regulates the activity of a transcription activator AREB1.” Proc. Nat. Acad. Sci. USA. 103(6): 1988-1993.

  37. Kamei, A., Seki, M., Umezawa, T., Ishida, J., Sato, M., Akiyama, K., Zhu, J.K. and Shinozaki, K. (2005) “Analysis of gene expression profiles in Arabidopsis salt-overly sensitive mutants sos2-1 and sos3-1.” Plant Cell Environ. 28(10): 1267-1275.

  38. Kitahata, N., Saito, S., Miyazawa, Y., Umezawa, T., Shimada, Y., Min, Y.K., Mizutani, M., Hirai, N., Shinozaki, K., Yoshida, S. and Asami T.(2005) “Chemical regulation of abscisic acid catabolism in plants by cytochrome P450 inhibitors.” Bioorg. Med. Chem. 13(14): 4491-4498.

  39. Umezawa, T., Yoshida, R., Maruyama, K., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2004) “SRK2C, a SNF1-related protein kinase 2 that improves drought tolerance by controlling stress-responsive genes in Arabidopsis thaliana.” Proc. Nat. Acad. Sci. USA. 101(49): 17306-17311. [Pubmed] [Free Full Text]

  40. Narusaka, Y., Narusaka, M., Seki, M., Umezawa, T., Ishida, J., Nakajima, M., Enju, A. and Shinozaki, K. (2004) “Crosstalk in the responses to abiotic and biotic stresses in Arabidopsis: Analysis of gene expression in cytochrome P450 gene superfamily by cDNA microarray.” Plant Mol. Biol. 55:327-342.

  41. Choi, P., Mano, Y., Ishikawa, A., Odashima, M., Umezawa, T., Fujimura, T., Takahata, Y. and Komatsuda, T. (2003) “Construction of a high-density AFLP and SSR map using recombinant inbred lines of cultivated × weedy soybean.” Breed. Sci. 53(4):335-344.

  42. Seki, M., Ishida, J., Narusaka, M., Fujita, M., Nanjo, T., Umezawa, T., Kamiya, A.,Nakajima, M., Enju, A., Sakurai, T., Satou, M., Akiyama, K., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y. and Shinozaki, K. (2002) “Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray.” Func. Integ. Genomics 2(6):282-91.

  43. Umezawa, T., Mizuno, K. and Fujimura, T. (2002) “Discrimination of genes expressed in response to the ionic or osmotic effect of salt stress in soybean with cDNA-AFLP.” Plant Cell Environ.25(12): 1617-1625.

  44. Umezawa, T., Shimizu, K., Kato, M. and Ueda, T. (2001) “Effects of non-stomatal components on photosynthesis in soybean under salt stress.” Jpn. J. Trop. Agric.45(1): 57-63.

  45. Umezawa, T., Shimizu, K., Kato, M. and Ueda, T. (2000) “Enhancement of salt tolerance in soybean with NaCl pretreatment.” Physiol. Plant.110: 59-63.

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Review/Books

  1. Kamiyama, Y., Katagiri, S. and Umezawa, T. (2021) "Growth promotion or osmotic stress response: How SNF1-related protein kinase 2 (SnRK2) kinases are activated and manage intracellular signaling in plants" Plants 10(7): 1443. [Journal]

  2. Kuromori, T., 　Mizoi, J., Umezawa, T., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2013) "Stress signaling networks: drought stress.” In The Plant Science (ed. S. Hoswell), Springer, Article ID:349838.[Link]

  3. Umezawa, T., Sugiyama, N., Anderson, J.C., Takahashi, F., Ishihama, Y., Peck, S.C., Shinozaki, K. (2013) "Protein phosphorylation networks in abscisic acid signaling.” In Plant and Microbe Adaptations to Cold in a Changing World (eds. R. Imai, M. Yoshida and N. Matsumoto), Springer, pp.155-164.

  4. Umezawa, T. (2011) “Systems biology approaches to abscisic acid signaling.” J. Plant Res. 24(4): 539-548.

  5. Umezawa, T., Hirayama, T., Kuromori, T. and Shinozaki, K. (2011) “Regulatory networks of plant responses to abscisic acid” Adv. Botan. Res. 57: 201-233.

  6. Umezawa, T., Nakashima K., Miyakawa, T. Kuromori, T. Tanokura, M., Shinozaki, K. and Yamaguchi-Shinozaki, K. (2010) “Molecular basis of the core regulatory network in ABA responses : sensing, signaling and transport.” Plant Cell Physiol. 51(11): 1821-1839.

  7. Hirayama, T. and Umezawa, T. (2010) “The PP2C–SnRK2 complex: The central regulator of an abscisic acid signaling pathway.” Plant Signal. Behav. 5(2): 160-163.

  8. Seki, M., Umezawa, T., Kim, J.M., Matsui, A., To, T. and Shinozaki, K. (2007) “Transcriptome analysis of plant drought and salt stress response.” In Advances in Molecular Breeding toward Drought and Salt Tolerant Crops (eds. M.A. Jenks, P.M. Hasegawa and S.M. Jain.), Springer Publishing, pp.261-283.

  9. Seki, M., Umezawa, T., Urano, K. and Shinozaki, K. (2007) “Regulatory metabolic networks in drought stress responses.”  Curr Opin Plant Biol.10(3): 296-302.

  10. Umezawa, T., Fujita, M., Fujita, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2006) “Engineering drought tolerance in plants: Discovering and tailoring genes unlock the future.”
    Curr. Opin. Biotech. 17: 113-122.

  11. Seki, M., Ishida, J., Nakajima, M., Enju, A., Iida, K., Satou, M., Fujita, M., Narusaka, Y., Narusaka, M., Sakurai, T., Akiyama, K., Oono, Y., Kamei, A., Umezawa, T., Mizukado, S., Maruyama, K., Yamaguchi-Shinozaki, K. and Shinozaki, K. (2005)　”Genomic analysis of stress response.” In Plant Abiotic Stress (eds. M.A. Jenks and P.M. Hasegawa), Blackwell Publishing Ltd, Sheffield, UK, pp.248-265.

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International conferences

  1. Umezawa, T. “Protein phosphorylation network in abscisic acid signaling.” 12th Annual Plant Biology Minisymposium, College Park, MD, Apr. 8-9, 2011. (Invited)

  2. Umezawa, T., Yamaguchi-Shinozaki, K. and Shinozaki, K. “Regulatory Network in Drought Stress Response and Tolerance : from Arabidopsis to crops.” International Symposium on Drought Tolerance in Plants, Goiania, Brazil, Oct. 19-21, 2010. (Invited)

  3. Umezawa, T., Sugiyama, N., Mizoguchi, M., Hayashi, S., Myouga, F., Yamaguchi-Shinozaki, K., Ishihama, Y., Hirayama, T. and Shinozaki, K. “The PP2C-SnRK2 complex : a central regulatory module in abscisic acid signaling.” Plant Protein Phosphorylation Symposium, University of Missouri-Columbia, Columbia, MO, May. 26-28, 2010. (Selected for oral presentation)
    　
  4. Umezawa T. “Type 2C Protein Phophatases Directly Regulate Abscisic Acid-Activated protein Kinases in Arabidopsis.” North Carolina Plant Molecular Biology Consortium Seminar Series, North Carolina Biotechnology Center, Research Triangle Park, NC, Nov. 2, 2009.

  5. Umezawa T. et al. ”Construction of a Soybean Full-length cDNA Library and Oligonucleotide Microarray.” The Kazusa Conference on Legume Genetics and Genomics in Asia, Kazusa DNA Research Institute, Kisarazu, Japan, Nov. 28-29, 2006

  6. Umezawa T., Yoshida R., Maruyama K., Yamaguchi-Shinozaki K. and Shinozaki K. “Arabidopsis SnRK2 protein kinases and osmotic stress signaling.” The Xth France Japan Workshop on Plant Sciences: Cellular Signalling and Development, Toulouse, France, Sep. 24-28, 2005.

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Patents

  1. Japan patent #2005-253395: Plant having tolerance to environmental stress ((Applicants: Taishi Umezawa and Kazuo Shinozaki)

  2. U.S. Patent #20050214808: Plant having tolerance to environmental stress (Applicants: Taishi Umezawa and Kazuo Shinozaki)

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