"Measurement of Mitochondrial Electron Transfer Complex Activity by Cell Imaging"

Welcome to Ohta Lab!
Ohta Lab is a laboratory in Department of Biotechnology and Life Science,
Graduate School of Engineering in Tokyo University of Agriculture and Technology.



        
 



Mitochondria are one of the intracellular organelles that play a central role in intracellular energy production, generation of reactive oxygen species, and cell death. We hope to contribute to human health by investigating and regulating its mechanism.


NEWS


2026/8/20  Student and teacher from Kashima Gakuen High School visited our laboratory.
After introducing our research, they attempted an experiment to isolate mitochondria from cells using a new method (iMIT).
Although it was their first time, they were able to obtain a mitochondrial suspension with high activity (approximately 85% of the mitochondria possessed a membrane potential).




 
 
2026/7/30  Our joint research with Dr. Yuma Yamada at the Graduate School of Pharmaceutical Sciences at Hokkaido University have been published in a paper.

"Trans MIT: microfluidically fabricated lipid nano capsules for robust intracellular delivery of mitochondria-derived components and metabolic enhancement"

Materials Today Advances. database



 
 
2026/6/6  Our joint research with Luca Science has been published.

"Bioenergetic benefits and therapeutic potential of storable mitochondria"
Biochem Biophys Res Commun. 828,154119, database



 
 
2026/3/25 Mr. Takada, Mr. Kishi and Mr. Wu have graduated.





 
2026/3/10,11  Mr. Tamura presented at the 15th Kanto Branch Meeting of the Biophysical Society of Japan (Waseda University, Nishiwaseda Campus).




 
 
2026/3/3 Mr. Izumisawa, Mr. Kojima, Mr. Sato, Mr. Joo and Ms. Mizukawa presented their graduation theses.

Juta Izumisawa
"Changes in Activity of Mitochondrial Electron Transport Chain Complex During Oxidative Damage"

Shoya Kojima
"Effects of Mitobroth on Oxidative Damage of Normal Human Skin Fibroblasts"

Yuki Sato
"Influence of Mitochondrial Damage on Mitochondrial Administration Effects"

Sasuke Joo
"Effects of Mitochondrial Supplementation on Oxidative Damage in Normal Human Epidermal Keratinocytes"

Runa Mizukawa
"Changes in Mitochondrial Electron Transport Chain Complex Activity During Neuronal Differentiation"


 
 
2026/2/5  Mr. Takada, Mr. Kishi and Mr. Wu presented their master's theses.

Ren Takada
"The Effect of Mitochondrial Supplementation: An Analysis Based on Mitochondrial Damage"

Kazuto Kishi
"Effects of Mitochondrial Administration on Hypoxia-Reoxygenation Injury in Cultured Cardiac Myocytes"

Wu Yujie
"Suppression of Cardiotoxicity by the Anticancer Drug Doxorubicin by Mitochondrial Addition"



 
2025/12/10

Saito-Ohta Lab won the TUAT Department of Biotechnology and Life Science Fall Softball Tournament 2025.





 
2025/11/3, 4, 5  Mr. Tamura, Mr. Kawase and Mr. Kato presented at the 98th Annual Meeting of the Japanese Biochemical Society (Kyoto International Conference Center).




 
2025/10/8   Third-year undergraduate students Ms. Kawakita, Ms. Nakao, Ms. Nakano, Ms. Mitsuhashi, Mr. Yokoyama joined our lab!



 
2025/9/26, 27  Prof. Ohta presented at the 31st Annual Meeting of Japan Bioimaging Society (Kumamoto Prefectural Exchange Center (Parea)).



 
2025/9/18  Mr. Ibban obtained a Ph. D.



 
2025/8/20
Mr. Ibban presented his doctor's theses.



 
2025/8/2 Mr. Ibban's paper has been published.

"Antioxidant Effects of Exogenous Mitochondria: The Role of Outer Membrane Integrity"
Antioxidants. 14(8), 951, database



 
2025/5
 

A paper on intact mitochondrial isolation methods has been published.

"A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality"
Biophysics and Physicobiology. 22, e220012_1, database



 
2024/12/4  Saito-Ohta Lab took 3rd place in the TUAT Department of Biotechnology and Life Science Fall Softball Tournament 2024.
 
 




 
2024/11/6
Mr. Ibban, Mr. Kishi, Mr. Takada and Mr. Wu presented at the 97th Annual Meeting of the Japanese Biochemical Society (PACIFICO Yokohama North).




 
2024/10
Third-year undergraduate students Mr. Izumisawa, Mr. Kojima, Mr. Sato, Mr. Joo, Ms. Mizukawa joined our lab!




 
2024/5/23 Prof. Ohta presented at The 39th Annual Meeting of the Academy of Pharmaceutical Science and Technology, Japan (Kobe International Conference Center).




 
2024/3/6
Ms. Neda presented at the 13th Kanto Branch Meeting of the Biophysical Society of Japan (Tokyo University of Science, Kagurazaka).




 
2024/3/1
 

Mr. Kishi, Mr. Kuroda and Mr. Takada presented their graduation theses.




 
2024/2/9
 

Ms. Naima presented her doctor's theses.



 
2024/2/8
 
Ms. Ogihara, Ms. Koyama, Ms. Saito, Mr. Maekawa and Ms. Neda presented their master's theses.



 
2024/1  Ms. Naima's paper has been published.

"A method for isolating and cryopreserving intact mitochondria with improved integrity and functionality"
Biophysics and Physicobiology. 22, e220012_1, database



 
2023/11/14
 

Ms. Kutami, Ms. Nishimura, Ms. Negoro, Mr. Ibban and Ms. Naima presented at the 61st Annual Meeting of the Biophysical Society of Japan (Nagoya Congress Center)




 
2023/10/31
 

Prof. Ohta presented at the 96th Annual Meeting of the Japanese Biochemical Society (Fukuoka International Congress Center, Marine Messe Fukuoka Hall B).



 
 
2023/10/1
 

Yujie Wu (research student) joined our lab!




 
2023/03/06

Ms. Isaka, Mr. Uchiumi, and Ms. Doi presented at the 12th Kanto Branch Meeting of the Biophysical Society of Japan (Tokyo University of Agriculture and Technology, Koganei).



2023/03/02

Ms. Kutami and Ms. Negoro presented their graduation theses.




2023/02/07

Ms. Isaka, Mr. Uchiumi, and Ms. Doi presented their master's theses.




2022/10/27

Prof. Ohta presented at the international conference "Targeting Mitochondria" (Berlin).



2022/10/06

Prof. Ohta gave a lecture at the 43rd Symposium on Interactions between Biomembranes and Drugs (Hokkaido University).



2022/10/01

Ibban Sadab Sipar (D1) joined our lab!




2022/09/28, 29

Ms. Ogihara, Ms. Koyama, Ms. Saito, Ms. Neda, Mr. Maekawa, and Ms. Naima presented at the 60th Annual Meeting of the Biophysical Society of Japan (Hakodate Arena).



2022/09/03

Prof. Ohta presented at the 31st Annual Meeting of Japan Bioimaging Society (Osaka University).



2022/08/31

Joint research with Prof. Yutaka Kuroda's group (Tokyo University of Agriculture and Technology) has been published.

“Direct Analysis of Mitochondrial Damage Caused by Misfolded/Destabilized Proteins”
Int. J. Mol. Sci. 23, 9881, database



2022/07/03 Joint research with Prof. Takeo Miyake's group (Waseda University) has been published.

“pH modulation in adhesive cells with a protonic biotransducer”
Bioelectrochemistry. 147, 108202, database



 
2022/04/04 

Joint research with Dr. Ikuro Osawa (Tokyo Metropolitan Institute of Gerontology) has been published in a press release. pdf



 
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