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The two Gtsf paralogs in silkworms orthogonally activate their partner PIWI proteins for target cleavage
The PIWI-interacting RNA (piRNA) pathway is a protection mechanism against transposons in animal germ cells. Most PIWI proteins possess …
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Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants
Secondary small interfering RNA (siRNA) production, triggered by primary small RNA targeting, is critical for proper development and …
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H3K4me3 histone modification in baculovirus-infected silkworm cells
Baculovirus infection modulates the chromatin states and gene expression of host insect cells. Here we performed chromatin …
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Dynamic subcellular compartmentalization ensures fidelity of piRNA biogenesis in silkworms
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CRISPR/Cas9-mediated mutagenesis of Ago2 and Siwi in silkworm cultured cells
The BmN-4 cell line, originated from the silkworm Bombyx mori ovary, possesses endogenous small interfering RNA (siRNA) and …
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Potential for small RNA production against Bombyx mori latent virus in Bombyx mori ovaries
Bombyx mori latent virus (BmLV) is a positive, single-stranded insect RNA virus closely related to plant maculaviruses. BmLV was first …
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Whole-genome sequencing and comparative transcriptome analysis of Bombyx mori nucleopolyhedrovirus La strain
o̧pyright 2020, Springer Science+Business Media, LLC, part of Springer Nature. The Bombyx mori nucleopolyhedrovirus (BmNPV) La is a …
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Zucchini consensus motifs determine the mechanism of pre-piRNA production
PIWI-interacting RNAs (piRNAs) of between approximately 24 and 31 nucleotides in length guide PIWI proteins to silence transposons in …
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Masc-induced dosage compensation in silkworm cultured cells
The Masculinizer (Masc) gene encodes a CCCH-tandem zinc finger protein that controls both masculinization and dosage compensation in …
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VCP Machinery Mediates Autophagic Degradation of Empty Argonaute
o̧pyright 2019 The Author(s) The Argonaute subfamily of proteins (AGO) loads microRNAs (miRNAs) to form the effector complex that …
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