KAWANO Lab.

Domain-specific deformation of phase-separated liposomes

Kayano Izumi and Ryuji Kawano
Biophysical Journal, 2024, 123 (3), 233a.

Time series analysis for detecting peptide fragments using EOF-enhanced nanopore

Misa Yamaji and Ryuji Kawano
Biophysical Journal, 2024, 123 (3), 292a.

Membrane permeation of antimicrobial peptides through asymmetric lipopolysaccharide lipid bilayer

Wakana Hashimoto and Ryuji Kawano
Biophysical Journal, 2024, 123 (3), 508a.

Rapid synthesis of de novo β-barrel peptide nanopores using cell-free expression

Shoko Fujita and Ryuji Kawano
Biophysical Journal, 2024, 123 (3), 163a.

Membrane-spanning DNA system: designing strand displacement across lipid bilayers

Sotaro Takiguchi and Ryuji Kawano
Proceedings of DNA, 2023, accepted.

Effects of excess DNA probes on DNA detection at femtomolar levels using a biological nanopore

Nanami Takeuchi and Ryuji Kawano
Proceedings of DNA, 2023, accepted.

Construction of a DNA Switching Circuit with Consciousness based on Integrated Information Theory

Fumika Kambara, Sotaro Takiguchi, Hiroki Watanabe, Masahiro Takinoue and Ryuji Kawano
Proceedings of DNA, 2023, accepted.

Nanopore sensing for nucleic acid detection at femtomolar levels without amplification

Nanami Takeuchi and Ryuji Kawano
MicroTAS, 2023, accepted.

Liposome Budding: Microfluidic generation of monodisperse liposomes

Jiajiu Ji and Ryuji Kawano
MicroTAS, 2023, accepted.

Theranostics molecular robot: detect a miRNA from tumor cells and generate the DNA drug in a liposome

Harune Suzuki, Ken Komiya, and Ryuji Kawano
MicroTAS, 2023, accepted.

Microfluidic Generation of Micro-soap Bubbles For Airborne Molecular Robot

Rina Takagi and Ryuji Kawano
MicroTAS, 2023, accepted.

Construction of nanopores using β-hairpin peptides synthesized by a cell-free system

Shoko Fujita, Miyu Fukuda, Ikuro Mizoguchi, Izuru Kawamura and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 367a.

Discrimination of cationic peptides using malaria translocon, EXP2, nanopores

Mitsuki Miyagi, Nina Kurokawa, Masafumi Yohda and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 438a.

Long-term survivable liposomes in seawater

Kayano Izumi, Keiichiro Koiwai and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 82a.

Ultra-low concentration detection of DNA without its amplification using a biological nanopore

Nanami Takeuchi and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 287a.

Analysis of direct transmembrane permeability of mono-amino acids by the planar membrane system

Kaiyi Zheng, Kayano Izumi and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 367a.

Unfolding of β-hairpin and α-helical peptides through a biological nanopore

Miyu Fukuda and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 439a.

Facilitating the nanopore detection of protein fragment with a neutral charge

Misa Yamaji and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 435a.

Electrical detection and analysis of exosomes by a glass capillary nanopore

Kohei Hayashi and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 438a.

De novo design of β-barrel nanopore with Gly-kink towards single-molecule detection

Ikuro Mizoguchi, Satomi Imai, Shuhei Yoshida, Yoshio Hamada, Yuto Suzuki, Yuzuha Araki, Kenji Usui, Izuru Kawamura and Ryuji Kawano
Biophysical Journal, 2023, 122 (3), 468a.

MicroRNA detection at femtomolar concentration using a probe-based nanopore technique

Nanami Takeuchi and Ryuji Kawano
Biophysical Journal, 2022, 121 (3), 223a.

Analysis of membrane transportation of cell penetrating peptides using lipid bilayer system

Chihiro Saito and Ryuji Kawano
Biophysical Journal, 2022, 121 (3), 223a.

De novo design of peptide nanopores with β-barrel structure synthesized by cell-free expression

Shoko Fujita, Ikuro Mizoguchi, Miyu Fukuda, Masataka Usami and Ryuji Kawano
Biophysical Journal, 2022, 121 (3), 222a.

Discrimination of polycationic peptides using a translocon EXP2 nanopore

Mitsuki Miyagi, Sotaro Takiguchi, Kazuaki Hakamada, Masafumi Yohda and Ryuji Kawano
Biophysical Journal, 2022, 121 (3), 467a.

De novo designed α-helix peptides which form barrel-stave nanopores

Masataka Usami, Yusuke Sekiya, Batsaikhan Mijiddorj, Izuru Kawamura and Ryuji Kawano
Biophysical Journal, 2022, 121 (3), 222a.

Design and Characterization of Enzyme-responsive Synthetic Ion Channels

Iiro Kiiski, Nanami Takeuchi, Alexandre Legrand, Reiko Sakaguchi, Kenji Usui, Shuhei Furukawa, Ryuji Kawano
Proceedings of MicroTAS, 2022, 366-367.

Molecular Basis of Femtomolar-nanopore-detection of DNAs with the Excess Complementary Probes

Nanami Takeuchi and Ryuji Kawano
Proceedings of MicroTAS, 2022, 1091-1092.

Toward Operation of Molecular Robot in the Air: Glass Capillary-based Generation of Reprosducible Soap Bubble

Rina Takagi, Sotaro Takiguchi and Ryuji Kawano
Proceedings of MicroTAS, 2022, 707-708.

Nanopore Decoding for DNA-computed Over and Under-expression of MicroRNA Patterns

Sotaro Takiguchi and Ryuji Kawano
Proceedings of DNA28, 2022, 74.

Direct Detection of DNA Methylation and Determination Intermediates using Biological Nanopore

Liu Ping and Ryuji Kawano
Proceedings of MicroTAS, 2021, 83-84.

Liposome Deformation Stimulated by Peptides

Kayano Izumi and Ryuji Kawano
Proceedings of MicroTAS, 2021, 369-370.

Femtomolar MicroRNA Detection by Diagnostic DNA System and Biological Nanopore

Nanami Takeuchi and Ryuji Kawano
Proceedings of MicroTAS, 2021, 727-728.

Lipid Membrane Deformation Induced by Transmembrane Peptides

Kayano Izumi, Keisuke Shimizu and Ryuji Kawano
Biophysical Journal, 2020, 118 (3), 231a.

Analyzing Single-Molecule Behavior of a Small Protein in Confined Nanospace of a Biological Nanopore

Misa Yamaji, Natsumi Takai, Mauro Chinappi and Ryuji Kawano
Biophysical Journal, 2020, 118 (3), 474a.

Development of Simple and Rapid Fabrications for Solid-State Nanopores

Natsumi Takai, Masaki Matsushita, Kan Shoji, Tei Maki and Ryuji Kawano
Biophysical Journal, 2020, 118 (3), 349a.

Construction of Programmable Nanopore using de novo Designed β-Sheet Peptide

Keisuke Shimizu, Shungo Sakashita, Yoshio Hamada, Kenji Usui, Batsaikhan Mijiddorj, Izuru Kawamura and Ryuji Kawano
Biophysical Journal, 2020, 118 (3), 474a.

Nanopore Decoding for Solution of Hamiltonian Path Problem in DNA Computing

Sotaro Takiguchi, Nanami Takeuchi and Ryuji Kawano
Proceedings of FNANO, 2020, 185-186.

Nanopore Decoding for DNA Computation with Parallel Self-Assembly

Sotaro Takiguchi and Ryuji Kawano
Proceedings of MicroTAS, 2020, 486-487.

Electrophysiological Analysis of Aβ42 ㏌ Planar Lipid Bilayer Imitating Nervous Cell-Membrane

Yuri Numaguchi, Keisuke Shimizu, Kaori Tsukakoshi, Kazunori Ikebukuro, and Ryuji Kawano
Proceedings of MicroTAS, 2019, 42-43.

Nanopore Decoding for MicroRNA Pattern of Cancer with DNA Computation

Nanami Takeuchi, Moe Hiratani, Asuka Tada, and Ryuji Kawano
Proceedings of MicroTAS, 2019, 186-187.

Specific Detection of Point-Mutation-Position using Biological Nanopore

Ping Liu, Keisuke Shimizu, and Ryuji Kawano
Proceedings of MicroTAS, 2019, 1692-1693.

Construction of Programmable Nanopore using β-Sheet Peptides

Keisuke Shimizu, Naoki Saigo, Shungo Sakashita, Yoshio Hamada, Kenji Usui, Batsaikhan Mijiddorj, Izuru Kawamura and Ryuji Kawano
Proceedings of MicroTAS, 2018, 202-203.

Simple Fabrication of a Solid-State Nanopore

Natsumi Takai, Masaki Matsushita, Kan Shoji, Tei Maki and Ryuji Kawano
Proceedings of MicroTAS, 2018, 570-571.

Liposome Deformation using a Transmembrane Peptide

Kayano Izumi, Naoki Saigo, and Ryuji Kawano
Proceedings of MicroTAS, 2018, 1406-1408.

Lipobot: Negative Chemotaxis by Osmotic Pressure

K. Shoji and R. Kawano
Proceedings of MicroTAS, 2017, 963-964.

MicroRNA Pattern Recognition for Bile Duct Cancer Using Programmable DNA and Biological Nanopore

M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 219-220.

Precise Recognition for Translocation of Single-Stranded DNA Through a Biological Nanopore Using Wavelet Transform

A. Tamotsu, M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 1250-1251.

Nanopore Probe: Analysis of Hofmeister Effect in Nanospace

M. Matsushita and R. Kawano
Proceedings of MicroTAS, 2017, 281-282.

Design of Programmable Nanopore by Using β-sheet Peptides

K. Shimizu, N. Saigo, Y. Sekiya, S. Sakashita, Y. Hamada, K. Usui, and R. Kawano
Proceedings of MicroTAS, 2017, 1549-1550.

Biological Nanopore Filter: Separation and Detection of Oligonuceotides

A. Tada, A. Tamotsu, M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 1328-1329.

A Single Molecular Logic Gate: “AND” Operation Using DNA Immobilized in Biological Nanopore

M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 118-119.

Recognition of microRNA Expression Pattern in Serum Using Programmable Droplet System for Cancer Diagnosis

M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1244-1245.

Direct Urinary Diagnosis System for Bladder Cancer: Fourier Analysis of microRNA Pattern From Nanopore Measurements

A. Tamotsu, M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 579-580.

Biological Nanopore Probe: Evaluation of Physicochemical Properties of DNA in Yocto (10-24) Litre Space

M. Matsushita, H. Watanabe, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1471-1472.

Exploer The Appropriate Biological Nanopore Selected from Pore Forming Protein Families

H. Watanabe, K. Tanaka, K. Tsumoto and R. Kawano
Proceedings of MicroTAS, 2016, 1304-1305.

A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine

Y. Sekiya, H. Watanabe, K. Usui and R. Kawano
Proceedings of MicroTAS, 2016, 595-596.

Molecular Evolution of Antimicrobial Peptides Using from Ascidiacea, Frog and Human

N. Saigo, Y. Sekiya, H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2016, 1511-1512.

Double-Stacking Lipid Bilayer Formation Using Five-Layerd Microchannels

K. Shoji and R. Kawano
Proceedings of MicroTAS, 2016, 723-724.

Electrical Analysis of The Pore-Forming Translocon Using Lipid Bilayers in Droplet System

K. Hakamada, H. Watanabe, R. Kawano, K Noguchi and M. Yohda
Proceedings of MicroTAS, 2016, 659-660.

Self-Assembled Nanoplates at The Water-Oil Interface

D. Ishikawa, Y. Suzuki, C. Kurokawa, M. Ohara, M. Morita, M. Yanagisawa, R. Kawano, M. Endo and M. Takinoue
Proceedings of MicroTAS, 2016, 116-117.

Stimuli-Responsive Microfiber Fabricated with Double-Network Hydrogel

S. Nakajima, R. Kawano and H. Onoe
Proceedings of MicroTAS, 2016, 1128-1129.

Logic Gate Operation using Three-way Junction DNA and Biological Nanopore

M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 1493-1495

Effects of Antimicrobial Activities on D- and L-Bombinin using Artificial Bacteria Cell-Membrane

Y. Sekiya, H. Watanabe, I. Kawamura, R.Kawano
Proceedings of MicroTAS, 2015, 823-825

Autonomous Diagnosis and Therapy: miRNA Detection and Drug Release using Programmable DNA and Biological Nanopore

M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 906-908

A Single Molecule Detection of Protein by Appropriate Sized Biological Nanopores

H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2015, 51-53

Extend The Size of Biological Nanopore Using Mgainin and Perforin Pores

H. Watanabe, R. Kawano
Proceedings of MicroTAS, 2014, 2125-2127.

DNA/RNA Computing with Biological Nanopore in Droplets System: AND Operation Using RNA Polymerization

M. Ohara, M. Takinoue, R. Kawano
Proceedings of MicroTAS, 2014, 1790-1792.

Logic Operation in DNA Nano Device: Electrical Input/Output through Biological Nanopores

K. Inoue, R. Kawano, H. Yasuga, M. Takinoue, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of MicroTAS, 2013, 1881-1883.

Droplet Network Connected By Biological Nanopores For DNA Computing

H. Yasuga, R. Kawano, M. Takinoue, Y. Tsuji, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of IEEE Transducers, 2013,1221-1222.

Synthetic Nanocage Formed by Rhodium-Organic Cuboctahedra: For Single Molecule Detection in Lipid Bilayer

R. Kawano, S. Furukawa, D. Kiriya, T. Osaki, K. Kamiya, S. Kitagawa, S. Takeuchi
Proceedings of IEEE Transducers, 2013, 854-855.

Confocal Laser Scanning Microscopic Observation of Deformation, Biological Reaction, and Contact of Cells using Mechanical Trapping System with Parylene Micro Filter

Y. Abe, K. Kamiya, T. Osaki, R. Kawano, N. Miki, S. Takeuchi
Proceedings of IEEE Transducers, 2013, 416-417.

Logic Gate Using Artificial Cell-Membrane: Nand Operation by Transmembrane DNA via a Biological Nanopore

H. Yasuga, R. Kawano, M. Takinoue, Y. Tsuji, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of IEEE MEMS, 2013, 1005-1006.

Intra/Extracellular Investigation for Ion Channels with Lipid Bilayer Array at the Single Molecule Level

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, K. Kamiya, T. Ide, N. Miki, S. Takeuchi
Proceedings of IEEE MEMS, 2013, 57-58.

Split-and-Contact Device to Form Planar Lipid Bilayers

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of IEEE MEMS, 2013, 161-164.

Microfluidic Analysis of ATP-Binding Cassette Transporters at Single-Vesicle Level

H Sasaki, R Kawano, T Osaki, K Kamiya, S Takeuchi
Proceedings of Biophysical Journal, 2012, 102 (3), 659a.

Solution Exchange of Droplet Contacting Lipid Bilayer System

Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of IEEE MEMS, 2012, 882-885.

Rapid Perfusion System for Inhibition Investigation of Membrane Proteins in Planar Lipid Bilayer

Y. Tsuji, R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of MicroTAS, 2012, 683-685.

Portable Membrane Protein Chip: Development of Membrane Protein sensors for Environment Analysis

R. Kawano, Y. Tsuji, T. Osaki, K. Kamiya, N. Miki, Y. Tanaka, S. Takeuchi
Proceedings of MicroTAS, 2012, 845-847.

Automated Drug Screening System for Ion Channel Proteins

R. Kawano, Y. Tsuji, M. Hirano, T. Osaki, H. Sasaki, K. Kamiya, N. Miki, T. Ide, S Takeuchi
Proceedings of MicroTAS, 2011, 76-78.

Parallel Recognition of Single-strand DNA using a Biological Nanopore Array

Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, K. Kamiya, N. Miki, S. Takeuchi
Proceedings of MicroTAS, 2011, 1284-1286.

Easy and Stable Lipid Bilayer Formation: A Droplets-Contacting-Method in Parylene Micropores for Multiple Ion Channel Recordings

Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, N. Miki, S. Takeuchi
Proceedings of the Transducers, 2011, 214-217.

Uniformly-Sized Giant Liposome Formation with Gentle Hydration

T. Osaki, K. Kuribayashi, R. Kawano, H. Sasaki, S. Takeuchi
Proceedings of the IEEE MEMS, 2011, 103-106.

25 Second Cocaine Sensing by Membrane Protein Channel Integrated in a Microfluidic Device

R. Kawano, T. Osaki, H. Sasaki, M. Takinoue, S. Yoshizawa, S. Takeuchi
Proceedings of the IEEE MEMS, 2011, 1333-1336.

Transporters on a chip: a fluorescence analysis of an ATP-binding cassette (ABC)-transporter

H. Sasaki, H. Onoe, T. Osaki, R. Kawano, S. Takeuchi
Proceedings of microTAS, 2010, 842-844.

Solvent-Free Bilayer Lipid Dome Device for Channel Protein Recordings

T. Osaki, R. Kawano, K. Kuribayashi, H. Sasaki, S. Takeuchi
Proceedings of microTAS, 2010, 540-542.

A parylene nanopore for stable planar lipid bilayer membrane

R. Kawano, T. Osaki, S. Takeuchi
Proceedings of the IEEE MEMS, 2010, 923-926.

Escape Dynamics of DNA from a Nanopore under the Influence of an AC Bias

Daniel K Lathrop, Geoffrey A Barrall, Eric N Ervin, Michael G Keehan, Michael A Krupka, Ryuji Kawano, Henry S White, Andrew H Hibbs
Proceedings of Biophysical Journal, 2009, 96 (3), 647a.