2026
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Molecular diagnostics for infectious disease and cancer based on glass capillary nanopore sensing
Ryogo Higashi, Izumi Shibayama, Jiajue Ji, Tao Ren, Iiro Kiiski, Nanami Takeuchi and Ryuji Kawano
Curr. Opin. Biotechnol., 2026, 97, 103394. DOI:10.1016/j.copbio.2025.103394
2025
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Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA
Soma Emura, †Nanami Takeuchi, Tomoko Ohshima, Kazuhito Satomura, and †Ryuji Kawano
ChemRxiv, 2025. DOI:10.26434/chemrxiv-2025-tcgk9
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De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs
Zugui Peng, Masataka Usami, Ayaka Nakada, Yusuke Sekiya, Shoko Fujita, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano
ACS Nano, 2025. DOI:10.1021/acsnano.5c15080
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DNA Origami Assembly inside Liposomes via Nanopore-Mediated DNA Transportation
Shoji Iwabuchi, Kengo Kurematsu, Kazuya Yokoo, Yusuke Sakai, Yoshihiro Shimizu, and Ryuji Kawano
ChemRxiv, 2025. DOI:10.26434/chemrxiv-2025-w3m81
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Self-Assembling Synthetic Peptides Remodel Mitochondrial Properties via Nanopore Formation
Nhung Thi Hong Nguyen, Shoko Fujita, Nanami Takeuchi, Ryuji Kawano, Takanari Inoue
BioRxiv, 2025. DOI:10.1101/2025.08.26.672490
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A kinase-responsive synthetic ion channel
†Nanami Takeuchi, †Iiro Kiiski, Shoko Fujita, Alexandre Legand, Tomoki Tateishi, Reiko Sakaguchi, Tomoko Inose, Kenji Usui, Shuhei Furukawa, Ryuji Kawano (†: equal contribution)
ChemRxiv, 2025. DOI:10.26434/chemrxiv-2025-b65h5
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De Novo Design of α-helical Peptide Nanopores for Single-molecule Detection using Helix Packing Motifs
Zugui Peng, Masataka Usami, Ayaka Nakada, Yusuke Sekiya, Shoko Fujita, Batsaikhan Mijiddorj, Izuru Kawamura, and Ryuji Kawano
ACS Nano, 2025, accepted.
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Self-assembled Block Copolymer Domains as Macromolecular Ion Transport Systems in Biological Membranes
Shunji Kosaka, Jokichi Fukushima, Nanami Takeuchi, Noriko Miyamoto, Liliana de Campo, Ryuji Kawano, and Tomoki Nishimura
Chem. Sci., 2025, 16, 16522-16533. DOI:10.1039/D5SC04256A
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Nanopore-Based Single-Molecule Logic Unit (sMOLU)
Sotaro Takiguchi, Nanami Takeuchi, Harune Suzuki, Masayuki Ohara, Ken Komiya and Ryuji Kawano
Small, 2025, 21, 34. DOI:10.1002/smll.202501560
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Determination of the position of DNA methylation and base modifications using a biological nanopore
Ping Liu, Masayuki Honda and Ryuji Kawano
Small Methods, 2025, 9, 8. DOI:10.1002/smtd.202401760 -
Serine clamp of Clostridium perfringens binary toxin BECb (CPILEb)-pore confers cytotoxicity and enterotoxicity
Toru Yoshida, Chie Monma, Yuki Ninomiya, Sotaro Takiguchi, Shoko Fujita, Yuto Uchida, Noriaki Sakoda, Vladimir A. Karginov, Jun-ichi Kishikawa, Tomohito Yamada, Ryuji Kawano & Hideaki Tsuge
Commun. Biol. 2025, 8, 1102. DOI: 10.1038/s42003-025-08519-5 -
Reconstitution of nanopores in bilayers of diblock and triblock copolymers
Hiroaki Kihara, Mikiyoshi Sato and Ryuji Kawano
Phys. Chem. Chem. Phys., 2025, 27, 12483-12493. DOI: 10.1039/D4CP04782F -
Generation of cell-sized liposome using laser-induced microjets
Jiajue Ji, Shuma Kawai, Rina Takagi, Keiichiro Koiwai, Ryuji Kawano, and Yoshiyuki Tagawa
Lab Chip, 2025, 25, 2644-2653. DOI:10.1039/D5LC00149H -
Redesign of Translocon EXP2 Nanopore for Detecting Peptide Fragments
Mitsuki Miyagi, Misa Yamaji, Nina Kurokawa, Masafumi Yohda, and Ryuji Kawano
Small Methods, 2025, 2401562. DOI: 10.1002/smtd.202401562 -
Harnessing DNA computing and nanopore decoding for practical applications: from informatics to microRNA-targeting diagnostics
Sotaro Takiguchi, Nanami Takeuchi, Vasily Shenshin, Guillaume Gines, Anthony J. Geno, Jeff Nivala, Yannick Rondelez, Ryuji Kawano
Chem. Soc. Rev., 2025, 54, 8-32. doi:10.1039/d3cs00396e -
Complex and non-sequential current signatures of a β-hairpin peptide confined in a nanopore
Misa Yamaji, Mauro Chinappi, Blasco Morozzo della Rocca, Kenji Usui, Ryuji Kawano
Anal. Chem. 2025, 97, 4, 2044–2051. DOI:10.1021/acs.analchem.4c04150 -
A microfluidics platform for simultaneous evaluation of sensitivity and side effects of anti-cancer drugs using a three-dimensional culture method
Yuki Kobayashi, Honoka Hashizume, Sotaro Takiguchi, Jiajue Ji, Ryuji Kawano, Keiichiro Koiwai, Haru Yamamoto, Mohamed Elbadawy, Tsutomu Omatsu, Amira Abugomaa, Masahiro Kaneda, Tatsuya Usui & Kazuaki Sasaki
Sci. Rep., 2025, 15, 39. DOI: 10.1038/s41598-024-84297-0
2024
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Microfluidic generation of cell-sized liposomes using a budding strategy
Jiajue Ji, Ryuji Kawano
Preprint in ChemRxiv 2024, DOI: 10.26434/chemrxiv-2024-4gnff-v2 -
De novo design of nanopores for single-molecule detection that incorporates α-helix peptides
Masataka Usami, Zugui Peng, Yusuke Sekiya, Batsaikhan Mijiddorj, Izuru Kawamura, Ryuji Kawano
Preprint in ChemRxiv 2024, DOI: 10.26434/chemrxiv-2024-wzql1 -
Controlled Self-Assembly of Vesicles by Electrospray Deposition
Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Kaori Kuribayashi-Shigetomi, Shoji Takeuchi
Small Structures. 2024;5(6). doi:10.1002/sstr.202300543 -
Long-term Stable Liposome Modified by PEG-lipid in Natural Seawater
Kayano Izumi, Jiajue Ji, Keiichiro Koiwai, Ryuji Kawano
ACS Omega 2024, 9, 9, 10958-10966. DOI: 10.1021/acsomega.3c10346 -
Lipid Vesicle-Based Molecular Robots
Zugui Peng, Shoji Iwabuchi, Kayano Izumi, Sotaro Takiguchi, Misa Yamaji, Shoko Fujita, Harune Suzuki, Fumika Kambara, Genki Fukasawa, Aileen Cooney, Lorenzo Di Michele, Yuval Elani, Tomoaki Matsuura, Ryuji Kawano
Lab on a Chip 2024, 24, 996-1029. DOI: 10.1039/D3LC00860F -
Real-time monitoring of the amyloid β1–42 monomer-to-oligomer channel transition using a lipid bilayer system
Yuri Numaguchi†, Kaori Tsukakoshi†, Nanami Takeuchi, Yuki Suzuki, Kazunori Ikebukuro, Ryuji Kawano (†equal contribution)
PNAS Nexus 2024, 3, 1, pgad437, DOI: 10.1093/pnasnexus/pgad437
2023
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The potential of nanopore technologies toward empowering biophysical research: Brief history, basic principle and applications
Hirohito Yamazaki, Zugui Peng, Ryuji Kawano, Kan Shoji
Biophysics and Physicobiology 2023, e210003, DOI: 10.2142/biophysico.bppb-v21.0003 -
Bottom-up creation of cell-free molecular systems: Basic research toward social implementation
Izuru Kawamura, Ryuji Kawano, Tomoaki Matsuura
Biophysics and Physicobiology 2023, e200042, DOI: 10.2142/biophysico.bppb-v20.0042 -
Structural fexibility of apolipoprotein E‑derived arginine‑rich peptides improves their cell penetration capability
Yuki Takechi‑Haraya, Takashi Ohgita, Akiko Usui, Kazuchika Nishitsuji, Kenji Uchimura, Yasuhiro Abe, Ryuji Kawano, Monika I. Konaklieva, Mart Reimund, Alan T. Remaley, Yoji Sato, Ken‑ichi Izutsu, Hiroyuki Saito
Scientific Reports 2023, 13, 19396, DOI: 10.1038/s41598-023-46754-0 -
Enhancement of cell membrane permeability by using charged nanoparticles and a weak external electric field
Hideya Nakamura, Takumi Okamura, Masaya Tajima, Ryuji Kawano, Misa Yamaji, Shuji Ohsaki, Satoru Watano
Physical Chemistry Chemical Physics 2023, 25, 32356-32363. DOI: 10.1039/D3CP03281G -
Simultaneous Recognition of Over- and Under-expressed MicroRNAs using Nanopore Decoding
Sotaro Takiguchi, Fumika Kambara, Mika Tani, Tsuyoshi Sugiura, Ryuji Kawano
Analytical Chemistry 2023, 95, 39, 14675–14685, DOI: 10.1021/acs.analchem.3c02560 -
Structure-activity relationship study of helix-stabilized antimicrobial peptides containing non-proteinogenic amino acids
Takahito Ito, Wakana Hashimoto, Nobumichi Ohoka, Takashi Misawa, Takao Inoue, Ryuji Kawano, Yosuke Demizu
ACS Biomaterials Science & Engineering 2023, 9, 8, 4654–4661, DOI: 10.1021/acsbiomaterials.3c00759 -
Nanopore Filter: A Method for Counting and Extracting Single DNA Molecules using a Biological Nanopore
†Asuka Tada, †Nanami Takeuchi, Kan Shoji, Ryuji Kawano
(†equal contribution)
Analytical Chemistry 2023, 95, 26, 9805–9812, DOI: 10.1021/acs.analchem.3c00573 -
Liposome deformation induced by membrane-binding peptides
Kayano Izumi, Chihiro Saito, Ryuji Kawano
Micromachines 2023, 14(2), 373, DOI: 10.3390/mi14020373 -
Cell-Free Expression of De Novo Designed Peptides That Form β-Barrel Nanopores
Shoko Fujita, Izuru Kawamura, Ryuji Kawano
ACS Nano 2023, 17, 4, 3358-3367. DOI: 10.1021/acsnano.2c07970
2022
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Pattern Recognition of microRNA Expression in Body Fluids using Nanopore Decoding at Sub-femtomolar Concentrations
Nanami Takeuchi, Moe Hiratani, Ryuji Kawano
JACS Au 2022, 2, 8, 1829-1838, DOI: 10.1021/jacsau.2c00117 -
Effect of hydrophobic moment on membrane interaction and cell penetration of apolipoprotein E-derived arginine-rich amphipathic α-helical peptides
Yuki Takechi-Haraya, Takashi Ohgita, Mana Kotani, Hiroki Kono, Chihiro Saito, Hiroko Tamagaki-Asahina, Kazuchika Nishitsuji,
Kenji Uchimura, Takeshi Sato, Ryuji Kawano, Kumiko Sakai-Kato, Ken-ichi Izutsu, and Hiroyuki Saito
Scientific Reports 2022, 12, 4959, DOI: 10.1038/s41598-022-08876-9 -
Single Polypeptide Detection using a Translocon EXP2 Nanopore
†Mitsuki Miyagi, †Sotaro Takiguchi, Kazuaki Hakamada, Masafumi Yohda, Ryuji Kawano
(†equal contribution)
Proteomics 2022, 22, 5-6, 2100070, DOI: 10.1002/pmic.202100070
(Contribution to a Special Issue on "Protein detection and characterization with nanopore sensors" ) -
De novo design of a nanopore for single-molecule detection that incorporates a β-hairpin peptide
Keisuke Shimizu, Batsaikhan Mijiddorj, Masataka Usami, Ikuro Mizoguchi, Shuhei Yoshida, Shiori Akayama, Yoshio Hamada,
Akifumi Ohyama, Kenji Usui, Izuru Kawamura, Ryuji Kawano
Nature Nanotechnology 2022, 17, 67-75,
DOI: 10.1038/s41565-021-01008-w
2021
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Simple Fabrication of Solid-State Nanopores on a Carbon Film
Natsumi Takai, Kan Shoji, Tei Maki, Ryuji Kawano
Micromachines 2021, 12, 1135, DOI: 10.3390/mi12091135
(Contribution to a Special Issue on "Frontiers in Micromachines in Japan") -
Single-cell RNA-seq analysis reveals penaeid shrimp hemocyte subpopulations and cell differentiation process
Keiichiro Koiwai, Takashi Koyama, Soichiro Tsuda, Atsushi Toyoda, Kiyoshi Kikuchi, Hiroaki Suzuki, Ryuji Kawano
eLife 2021, 10, e66954, DOI: 10.7554/eLife.66954 -
Nanopore Decoding for a Hamiltonian Path Problem
Sotaro Takiguchi and Ryuji Kawano
Nanoscale 2021, 13, 6192-6200, DOI: 10.1039/D0NR09031J -
Lipid bilayer on a microdroplet integrated with a patterned Ag/AgCl microelectrode for voltage-clamp fluorometry of membrane transport
Taishi Tonooka, Toshihisa Osaki, Koji Sato, Ryuji Kawano, Shoji Takeuchi
Sensors and Actuators B: Chemical 2021, 334, 129643, DOI: 10.1016/j.snb.2021.129643 -
Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence
Motoharu Hirano, Chihiro Saito, Hidetomo Yokoo, Chihiro Goto, Ryuji Kawano, Takashi Misawa, Yosuke Demizu
Molecules 2021, 26(2), 444, DOI: 10.3390/molecules26020444
2020
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Rational Design of Helix-stabilized Antimicrobial Peptide Foldamers Containing α,α-Disubstituted Amino Acids or Side-Chain Stapling
Motoharu Hirano, Chihiro Saito, Chihiro Goto, Hidetomo Yokoo, Ryuji Kawano, Takashi Misawa, and Yosuke Demizu
ChemPlusChem 2020, 85, 12, 2731-2736, DOI: 10.1002/cplu.202000749 -
Analytical Model for Particle Capture in Nanopores Elucidates Competition among Electrophoresis, Electroosmosis, and Dielectrophoresis
Mauro Chinappi, Misa Yamaji, Ryuji Kawano, and Fabio Cecconi
ACS Nano 2020, 14, 11, 15816-15828, DOI: 10.1021/acsnano.0c06981 -
Microfiber-shaped programmable materials with stimuli-responsive hydrogel
Nobuki Takeuchi, Shunsuke Nakajima, Koki Yoshida, Ryuji Kawano, Yutaka Hori, and Hiroaki OnoeSoft Robotics 2020, 0, 0, DOI: 10.1089/soro.2020.0038
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Recent Advances in Liposome-Based Molecular Robots
Kan Shoji and Ryuji Kawano
Micromachines 2020, 11(9), 788, DOI: 10.3390/mi11090788 -
Analysis of membrane protein de-insertion-associated currents with nanoneedle-supported bilayers to discover pore formation mechanisms
Kan Shoji, Ryuji Kawano, and Ryan J. White
Langmuir 2020, 36, 34, 10012–10021, DOI: 10.1021/acs.langmuir.0c00833 -
Microfluidic formation of honeycomb-patterned droplets bounded by interface bilayers via bimodal molecular adsorption
Shougo Fujiwara, Kan Shoji, Chiho Watanabe, Ryuji Kawano, Miho Yanagisawa
Micromachines 2020, 11(7), 701, DOI: 10.3390/mi11070701 -
Recessed Ag/AgCl Microelectrode-Supported Lipid Bilayer for Nanopore Sensing
Kan Shoji, Ryuji Kawano, and Ryan J. White
Analytical Chemistry 2020, 92, 15, 10856-10862, DOI: 10.1021/acs.analchem.0c02720 -
Recognition of Single-Point-Mutation using a Biological Nanopore
Ping Liu and Ryuji Kawano
Small Methods 2020, 2000101, DOI: 10.1002/smtd.202000101 -
Enhancement of direct membrane penetration of arginine-rich peptides by polyproline II helix structure
Takashi Ohgita, Yuki Takechi-Haraya, Keisuke Okada, Saki Matsui, Misaki Takeuchi, Chihiro Saito, Kazuchika Nishitsuji, Kenji Uchimura, Ryuji Kawano, Koki Hasegawa, Kumiko Sakai-Kato, Kenichi Akaji, Ken-ichi Izutsu, Hiroyuki Saito
BBA - Biomembranes 2020, 183403, DOI: 10.1016/j.bbamem.2020.183403 -
Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems
Yoko Tanaka, Yu Kitamura, Ryuji Kawano, Kan Shoji, Moe Hiratani, Tetsuo Honma, Hikaru Takaya, Hirofumi Yoshikawa, Takaaki Tsuruoka, Daisuke Tanaka
Chem. Eur. J. 2020, 26, 41, 8889-8896, DOI: 10.1002/chem.202002105 -
Dynamic behavior of an artificial protein needle contacting a membrane observed by high-speed atomic force microscopy
Takafumi Ueno, Kento Niwase, Daisho Tsubokawa, Kosuke Kikuchi, Natsumi Takai, Tadaomi Furuta, Ryuji Kawano, Takayuki Uchihashi
Nanoscale 2020, 12(15), 8166-8173, DOI: 10.1039/d0nr01121e -
Biological Nanopore Probe: Probing of Viscous Solutions in a Confined Nanospace
Masaki Matsushita, Kan Shoji, Natsumi Takai, Ryuji Kawano
J. Phys. Chem. B., 2020, 124, 12, 2410-2416, DOI:10.1021/acs.jpcb.9b11096
2019
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Osmotic-Engine-Driven Liposomes in Microfluidic Channels
Kan Shoji and Ryuji Kawano
Lab on a Chip, 2019, 19, 3472-3480, DOI :10.1039/C9LC00788A -
DNA origami nanoplate-based emulsion with designed nanopore function
Daisuke Ishikawa, Yuki Suzuki, Chikako Kurokawa, Masayuki Ohara, Misato Tsuchiya, Masamune Morita, Miho Yanagisawa,Masayuki Endo, Ryuji Kawano, Masahiro Takinoue
Angew. Chem., Int. Ed., 2019, 58, 43, DOI : 10.1002/anie.201908392 -
Electrophysiological Analysis of Antimicrobial Peptides in Diverse Species
Naoki Saigo, Kayano Izumi, and Ryuji Kawano
ACS Omega, 2019, 4, 8, 13124-13130, DOI:10.1021/acsomega.9b01033 -
Electrophysiological Analysis of Membrane Disruption by Bombinin and its Isomer using Lipid Bilayer System
Yusuke Sekiya, Keisuke Shimizu, Yuki Kitahashi, Akifumi Ohyama, Izuru Kawamura, and Ryuji Kawano
ACS Appl. Bio. Mater., 2019, 2, 4, 1542-1548, DOI:10.1021/acsabm.8b00835 -
Spatially Resolved Chemical Detection with a Nanoneedle-Probe-Supported Biological Nanopore
Kan Shoji, Ryuji Kawano, and Ryan J. White
ACS Nano, 2019, 13(2), 2606-2614, DOI :10.1021/acsnano.8b09667
2018
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Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system
Koki Kamiya, Toshihisa Osaki, Kenji Nakao, Ryuji Kawano, Satoshi Fujii, Nobuo Misawa, Masatoshi Hayakawa, and Shoji Takeuchi
Scientific Reports, 2018, 17498, DOI:10.1038/s41598-018-35316-4 -
Nanopore Decoding of Oligonucleotides in DNA Computing
Ryuji Kawano
Biotechnology Journal, 2018, 1800091, DOI: 10.1002/biot.201800091 -
Spring-shaped stimuli-responsive hydrogel actuator with large deformation
Koki Yoshida, Shunsuke Nakajima, Ryuji Kawano, Hiroaki Onoe
Sensors and Actuators B-Chemical, 2018, 272, 361, DOI: 10.1016/j.snb.2018.05.174 -
Microfluidic Formation of Double-stacked Lipid Bilayers by Controlling the Water-Oil Interface
Kan Shoji and Ryuji Kawano
Micromachines, 2018, 9(5), 253, DOI: 10.3390/mi9050253 -
Channel Current Analysis Estimates the Pore-formation and the Penetration of Transmembrane Peptides
Yusuke Sekiya, Shungo Sakashita, Keisuke Shimizu, Kenji Usui and Ryuji Kawano
Analyst, 2018, 143(15), 3540-3543, DOI: 10.1039/C8AN00243F -
DNA Logic Operation with Nanopore Decoding To Recognize MicroRNA Patterns in Small Cell Lung Cancer
Moe Hiratani and Ryuji Kawano
Analytical Chemistry, 2018, 90(14), 8531-8537, DOI: 10.1021/acs.analchem.8b01586 -
Design of protein-responsive micro-sized hydrogels for self-regulating microfluidic systems
Mayu Hirayama, Kazuhiro Tsuruta, Akifumi Kawamura, Masayuki Ohara, Kan Shoji, Ryuji Kawano and Takashi Miyata
Journal of Micromechanics and Microengineering, 2018, 28(3), DOI: 10.1088/1361-6439/aaa876 -
Well-controlled cell-trapping systems for investigating heterogeneous cell-cell interactions
Koki Kamiya, Yuta Abe, Kosuke Inoue, Toshihisa Osaki, Ryuji Kawano, Norihisa Miki and Shoji Takeuchi
Advanced Healthcare Materials, 2018, 7(6), 2192-2659, DOI: 10.1002/adhm.201701208 -
Synthetic Ion Channels and DNA Logic Gates as Components of Molecular Robots (Invited Minireview)
Ryuji Kawano
ChemPhysChem, 2018, 19(4), 359-366, DOI: 10.1002/cphc.201700982
2017
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Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores
Hirokazu Watanabe, Alberto Gubbiotti, Mauro Chinappi, Natsumi Takai, Koji Tanaka, Kouhei Tsumoto and Ryuji Kawano
Analytical Chemistry, 2017, 89(21), 11269–11277, DOI: 10.1021/acs.analchem.7b01550 -
MicroRNA Detection at Femtomolar Concentration by a Biological Nanopore with an Isothermal Amplification
Haolin Zhang, Moe Hiratani, Kentaro Nagaoka and Ryuji Kawano
Nanoscale, 2017, 9, 16124-16127, DOI: 10.1039/C7NR04215A -
pH Regulates Pore Formation of a Protease Activated Vip3Aa from Bacillus thuringiensis
Thittaya Kunthic, Hirokazu Watanabe, Ryuji Kawano, Yoshikazu Tanaka, Boonhiang Promdonkoy, Min Yao and Panadda Boonserm
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2017, 1859, 2234-2241, DOI: 10.1016/j.bbamem.2017.08.018 -
Serial DNA Relay in DNA Logic Gates by electrical Fusion and Mechanical Splitting of Droplets
Hiroki Yasuga, Kosuke Inoue, Ryuji Kawano, Masahiro Takinoue, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
PLOS ONE, 2017, 12(7), DOI: 10.1371/journal.pone.0180876 -
Nanopore Logic Operation with DNA to RNA Transcription in a Droplet System
Masayuki Ohara, Masahiro Takinoue and Ryuji Kawano
ACS Synthetic Biology, 2017, 6(7), 1427-1432, DOI: 10.1021/acssynbio.7b00101 -
Stimuli-responsive Hydrogel Microfibers with Controlled Anisotropic Shrinkage and Cross-sectional Geometries
Shunsuke Nakajima, Ryuji Kawano and Hiroaki Onoe
Soft Matter, 2017, 13, 3710-3719, DOI: 10.1039/C7SM00279C -
Metal-Organic Cuboctahedra for Synthetic Ion Channels with Multiple Conductance States
Ryuji Kawano, Nao Horike, Yuh Hijikata, Mio Kondo, Arnau Carné-Sánchez, Patrick Larpent, Shuya Ikemura, Toshihisa Osaki, Koki Kamiya, Susumu Kitagawa, Shoji Takeuchi and Shuhei Furukawa
Chem, 2017, 2, 393-403, DOI: http://dx.doi.org/10.1016/j.chempr.2017.02.002 -
Amplification and quantification of an antisense oligonucleotide from target microRNA using programmable DNA and a biological nanopore
Moe Hiratani, Masayuki Ohara and Ryuji Kawano
Analytical Chemistry, 2017, 89(4), 2312-2317, DOI: 10.1021/acs.analchem.6b03830 -
Expression and characterization of the Plasmodium translocon of exported proteins component EXP2
Kazuaki Hakamada, Hirokazu Watanabe, Ryuji Kawano, Keiichi Noguchi and Masafumi Yohda
Biochemical and Biophysical Research Communications (BBRC), 2017, 482(4), 700-705, DOI:10.1016/j.bbrc.2016.11.097
2016
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Rhodium–Organic Cuboctahedra as Porous Solids with Strong Binding Sites
Shuhei Furukawa, Nao Horike, Mio Kondo, Yuh Hijikata, Arnau Carné-Sánchez, Patrick Larpent, Nicolas Louvain, Stéphane Diring, Hiroshi Sato, Ryotaro Matsuda, Ryuji Kawano and Susumu Kitagawa
Inorganic Chemistry, 2016, 55 (21), 10843–10846, DOI: 10.1021/acs.inorgchem.6b02091 -
Scalable fabrication of microneedle arrays via spatially controlled UV exposure
†Hidetoshi Takahashi, †Yun Jung Heo, Nobuchika Arakawa, Tesuo Kan, Kiyoshi Matsumoto, Ryuji Kawano and Isao Shimoyama (†equal contribution)
Microsystems & Nanoengineering, 2016, 2, 16049, DOI:10.1038/micronano.2016.49 -
Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, Kazunari Akiyoshi and Shoji Takeuchi
Nature Chemistry, 2016, 8, 881–889, DOI:10.1038/nchem.2537 -
Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array
Masayuki Ohara, Yusuke Sekiya and Ryuji Kawano
Electrochemistry, 2016, 84(5), 338-341, DOI: 10.5796/electrochemistry.84.338 -
Logic Gate Operation by DNA Translocation through Biological Nanopores
†Hiroki Yasuga,† Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
(†equal contribution)
PLoS One., 2016, 11(2):e0149667, DOI: 10.1371/journal.pone.0149667 -
Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, using the Droplet Contact Method
Hirokazu Watanabe and Ryuji Kawano
Analytical Sciences, 2016, 32(1), 57-60, DOI: 10.2116/analsci.32.57
2015
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Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins
Y. Abe, K. Kamiya, T. Osaki, H. Sasaki, R. Kawano, N. Miki and S. Takeuchi
Analyst, 2015, 140, 5557-5562, DOI: 10.1039/C4AN02201G -
Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications
M. Tani, R. Kawano, K. Kamiya & K. Okumura
Scientific Reports, 2015, 5, 10263, DOI: 10.1038/srep10263 -
Preparation of Molecule-Responsive Microsized Hydrogels via Photopolymerization for Smart Microchannel Microvalves
Y. Shiraki, K. Tsuruta, J. Morimoto, C. Ohba, A. Kawamura, R. Yoshida, R. Kawano, T. Uragami and T. Miyata
Macromol. Rapid Commun., 2015, 36: 515–519, DOI: 10.1002/marc.201400676 -
Preparation of Structurally Colored, Monodisperse Spherical Assemblies Composed of Black and White ColloidalParticles using a Micro Flow-Focusing Device
M. Teshima, T. Seki, R. Kawano, S. Takeuchi, S. Yoshioka and Y. Takeoka
Journal of Materials Chemistry C, 2015, 3, 769 - 777, DOI: 10.1039/C4TC01929F
2014
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A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
R. Kawano, Y. Tsuji, K. Kamiya, T. Kodama, T. Osaki, N. Miki, S. Takeuchi
PLoS ONE, 2014, 9(7): e102427, DOI: 10.1371/journal.pone.0102427 -
Lipid bilayers on a picoliter microdroplet array for rapid fluorescence detection of membrane transport
T. Tonooka, K. Sato, T. Osaki, R. Kawano, S. Takeuchi
Small, 2014, 10, No. 16, 3275–3282, DOI: 10.1002/smll.201470093 (Selected as the cover of issue)
2013
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Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
(†equal contribution)
Anal. Chem., 2013, 85, 10913-10919. -
Automated Parallel Recordings of Topologically Identified Single Ion Channels
R. Kawano, Y. Tsuji, K. Sato, T. Osaki, K. Kamiya, M. Hirano, T. Ide, N. Miki, S. Takeuchi
Scientific Reports, 2013, 3: 1995, DOI: 10.1038/srep01995. -
Round-Tip Dielectrophoresis-Based Tweezers for Single Micro-Object Manipulation
T. Kodama, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi
Biosensors and Bioelectronics, 2013, 47, 206-212. -
Droplet based lipid bilayer system integrated with microfluidic channels for solution exchange
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
(†equal contribution)
Lab on a Chip, 2013, 13, 1476-1481. (Selected as the inside cover of the issue)
2012
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A glass fiber sheet-based electroosmotic lateral flow immunoassay for point-of-care testing
Y. Oyama, T. Osaki, K. Kamiya, R. Kawano, T. Honjoh, H. Shibata, T. Ide, S. Takeuchi
Lab on a Chip 2012, 12, 5155-5159. (Selected as the inside cover of the issue) -
Microfluidic Control of the Internal Morphology in Nanofiber-based Macroscopic Cables
D. Kiriya, R. Kawano, H. Onoe, S. Takeuchi
Angewandte Chemie Int. Ed., 2012, 51, 7942-7947. (Selected as the inside cover of the issue) -
Single-vesicle estimation of ATP-binding cassette transporters in microfluidic channels
H. Sasaki, R. Kawano, T. Osaki, K. Kamiya, S. Takeuchi
Lab on a Chip 2012, 12, 702-704.
2011
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Simple and Stable Lipid Bilayer Formation: A Droplets Contacting Method using Parylene Micropores for Multiple Ion Channel Recordings (in Japanese)
Y. Tsuji, R. Kawano, T. Osaki, H. Sasaki, N. Miki, S. Takeuchi
IEEJ Trans.E. 2011, 131, 419-424. -
Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip
R. Kawano, T. Osaki, H. Sasaki, M. Takinoue, S. Yoshizawa, S. Takeuchi
J. Am. Chem. Soc., 2011, 133, 8474-8477. -
Electrical Access to Lipid Bilayer Membrane Microchambers for Transmembrane Analysis
T. Osaki, Y. Watanabe, R. Kawano, H. Sasaki, S. Takeuchi
Journal of Microelectromechanical Systems, 2011, 20, 797-799.
2010
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Lipid-coated Microdroplet Array for in vitro Protein Synthesis
T. Osaki, S. Yoshizawa, R. Kawano, H. Sasaki, S. Takeuchi
Anal. Chem. 2010, 83, 3186-3191. -
Quartz Nanopore Membranes for Suspended Bilayer Ion Channel Recordings
A. Schibel, T. Edwards, R. Kawano, W. Lan, and H. White
Anal. Chem. 2010, 82, 7259–7266. -
Parylene-coating in PDMS channels to prevent the absorption of fluorescent dyes
H. Sasaki, H. Onoe, T. Osaki, R. Kawano, S. Takeuchi
Sensors & Actuators: B. Chemical 2010 150, 478-482. -
A rupture detection algorithm for the DNA translocation detection through biological nanopore
T. Osaki, J. P. Barbot, R. Kawano, H. Sasaki, O. Francais, B. Le Pioufle, S. Takeuchi
Procedia Engineering 2010 5, 796-799. -
A Polymer-based Nanopore-integrated Microfluidic Device for Generating Stable Bilayer Lipid Membranes
R. Kawano, T. Osaki, H. Sasaki, S. Takeuchi
Small 2010, 6, 2100-2104. (selected as the inside cover of the issue) -
Monitoring the Escape of DNA from a Nanopore Using an Alternating Current Signal
D. K. Lathrop, E. N. Ervin, G. A. Barrall, M. G. Keehan, R. Kawano, M. A. Krupka, H. S. White, and A. H. Hibbs
J. Am. Chem. Soc. 2010, 132, 1878-1885. -
Solid-State Dye-Sensitized Solar Cells using Polymerized Ionic Liquid Electrolyte and Platinum-Free Counter Electrode
R. Kawano*, T. Katakabe, H. Shimosawa, M. K. Nazeeruddinn, M. Gratzel, M. Watanabe
Phys. Chem. Chem. Phys., 2010, 12, 1916-1921.
2009
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Controlling the Translocation of Single-Stranded DNA through α-Hemolysin Ion Channels Using Viscosity
R. Kawano*, A. Schibel, C. Cauley, H. S. White
Langmuir, 2009, 25 (2), 1233–1237.
2008
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Simultaneous Alternating and Direct Current Readout of Protein Ion Channel Blocking Events using Glass Nanopore Membranes
E. N. Ervin, R. Kawano, R. J. White, H. S. White
Anal. Chem. 2008, 80, 2069-2076.
2007
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Acceleration of Redox Diffusion and Charge Transfer Rates in an Ionic Liquid with Nanoparticles Addition
T. Katakabe, R. Kawano, M. Watanabe
Electrochem. Solid-State Lett., 2007, 10, F23-F25. -
Dye-Sensitized TiO2 Solar Cells using Imidazolium-Type Ionic Liquid Crystal systems as Effective Electrolytes
N. Yamanaka, R. Kawano, W. Kubo, N. Masaki, T. Kitamura, Y. Wada, M. Watanabe, S. Yanagida
J. Phys. Chem. B 2007, 111, 4763-4769 (Most-Accessed Article: 9th, 2007). -
Amphiphilic ruthenium dye as an ideal sensitizer in conversion of light to electricity using ionic liquid crystal electrolyte
R. Kawano*, Md.K. Nazeeruddin, A. Sato, M. Grätzel, M. Watanabe
Electrochem. Commun. 2007, 9, 1134-1138.
2006
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Specific Charge Transport in Ionic Liquids and Ion Gels and the Importance in Material Science
R. Kawano, H. Tokuda, T. Katakabe, H. Nakamoto, H. Kokubo, S. Imabayashi, M. Watanabe
Jpn. J. Polym. Sci. Technol., 2006, 63, 31-40. (Review, in Japanese).
2005
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Anomaly of Charge Transport of an Iodide/Tri-iodide Redox Couple in an Ionic Liquid and Its Importance in Dye-sensitized Solar Cells
R. Kawano, M. Watanabe
Chem. Commun. 2005, 2107-2109. -
Ionic Liquid Crystal as a Hole Transport Layer of Dye-Sensitized Solar Cells
N.Yamanaka, R. Kawano, W. Kubo, T. Kitamura, Y. Wada, M. Watanabe, S. Yanagida
Chem. Commun., 2005 , 740-742. (Selected as a Hot Article)
2004
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Dye-sensitized Solar Cells Using Ionic Liquid-based Electrolytes
H. Matsui, K. Okada , N. Tanabe, R. Kawano and M. Watanabe
Trans. Mater. Res. Soc. Jpn., 2004, 29, 1017-1020. -
Application of an Ionic Liquid-Based Electrolyte to a 100x100 mm2 Sized Dye-Sensitized Solar Cell
H. Matsui, K. Okada, T. Kawashima, T. Ezure, N. Tanabe, R. Kawano and M. Watanabe
J. Photochem. Photobio. A, Chem., 2004, 164, 129-135. -
High Performance Dye-Sensitized Solar Cells using Ionic Liquids as Their Electrolytes
R. Kawano, H. Matsui, C. Matsuyama, A. Sato, M. A. B. H. Susan, N. Tanabe, M. Watanabe
J. Photochem. Photobio. A, Chem., 2004, 164, 87-92.
2003
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Equilibrium Potentials and Charge Transport of an I-/I3- Redox Couple in an Ionic Liquid
R. Kawano, M. Watanabe
Chem. Commun. 2003, 330-331.
2000
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Intramolecular Magnetic Interaction of Phenylene-Linked Bis-β-diketone Metal Complexes
M. M. Matsushita, T. Yasuda, R. Kawano, T. Kawai, T. Iyoda
Chemistry Letters, 2000, No.7, 812-813.
