Oct. 13, 2017
A study about detection method for ultra low concentration microRNA conducted by Hiratani-san and Zhang-san was press released from TUAT.
Oct. 5, 2017
A paper about protein detection nanopore conducted by Watanabe-kun, Takai-san and Dr. Mauro (Univ. of Rome) was accepted in Analytical Chemistry.
Sep. 25, 2017
A paper about the detection of fM miRNAs conducted by Hiratani-san, Zhang-san(Veterinary Medicine, TUAT) and Dr. Nagaoka(Veterinary Medicine, TUAT) was accepted in RSC Nanoscale.
Sep. 25, 2017
The 35th Summer School of Electrochemical Society organized by our laboratory was held. Shimizu-kun and Shoji-san had nice work! And finally, Hiratani-san won a poster award as usual!
Sep. 22, 2017
2nd Japan-Korea International Symposium on Cyborgnics: Integration between cell and electronics was held in Beppu. Hiratani-san won "BEST POSTER PRESENTATION AWARD". Thank you very much for all the participants!
Sep. 10, 2017
Dr. Kawano organized a special event "State of the art of Medical Diagnostic Technology Using Biological Functions" and gave a talk on there@Nagasaki Univ. The title was "Development of Artificial Cell Membrane System to Medical Diagnosis Technology".
Sep. 9, 2017
Dr. Kawano gave an invited talk on special symposium "State of the art of Nano-micro Chemical Analysis" at the 66th Japan Analytical Chemistry@Katsushika campus of Tokyo univ. of science. The title was "Nano-micro Chemical Analysis Using Artificial Cell Membrane System".
Sep. 1, 2017
Laboratory camp was held in Minakami, Gunma prefecture. In this year, we exercised downhill and canyoning.
Aug. 31, 2017
The "Liquid Biopsy" shared written by Tamotsu-kun, Hiratani-san and Dr. Kawano was published from CMC.
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure under a low
energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding the nano
scale structure produced by living things in artifacts.
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro
electro mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in
lipid bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography in a clean room.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> Application: drug testing, food inspection, and diagnostic pathology.
The mimetic device of cellular information transfer mechanism
> Bottom-up construction with biological molecules for “Molecular robot”.
> Construction of logic circuits modeled after living cells by networking droplets.
> Large-scale parallel computation of the droplet system by combining chemical sensing
Electrical molecular computing using nanopores
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule
Reconstitution membrane proteins in lipid bilayers
> Antimicrobial peptides kill mycetocyte by forming nanopore into the cell membranes.
> Pore-forming of the antimicrobial peptide is measured electrically using artificial cell
> Clarification of pore-forming mechanism of the antimicrobial peptide and its application
of antimicrobial drugs are expected.
High-throughput measurement and preparation of droplets
J. Mater, Chem. C, 2015, 3, 769–777
Tani, M. et al., Sci. Rep., 2015, 5, 10263
> Microfluidic enables energy and sample saving in chemical analysis by microscale
operation or chemical reaction on a microchip.
> The flow velocity in micro channels can be controlled precisely.
> The simultaneous chemical reaction is caused by flowing into some reaction fields
> The control of a flux in an oil layer and a water layer enables to form the uniform-
Ryuji Kawano, Ph.D.
Associate Professor( tenure-track )
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
The master's course
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
Favorites : Chorus, Music, Mah‐jong
Favorites : Theatergoing, Savings
Favorites : American Football
Favorites : Electrical work
Favorites : Watching soccer, Aikido
Favorites : Soft Tennis
Favorites : Softball, Reading
Favorites : Cooking, Photo
Kan Shoji, Ph.D.
PRESS & AWARD
May 5, 2017 The achievement of the study about nanopore DNA logic operation was press released from TUAT.
Mar. 3, 2017 The collaborating research with Prof. Furukawa (Kyoto Univ.) was published in Mynavi News.
Feb. 10, 2017 Hiratani-san's study about theranostics was press released from TUAT!
Oct. 24, 2016 Shoji-san won the 2016th research grant from The Okawa Foundation for Information and
Oct. 19, 2016 The study about miRNA nanopore detection of Hiratani-san was appeared in the Nikkei Business Daily!
Oct. 9-14, 2016 microTAS 2016@ Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS
Young Researcher Poster Award.
Feb. 23, 2016 Oral presentation for Graduation theses of B4, Hiratani-san got the pest presentation award.
Feb. 12, 2016 Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an
Oct. 25 - 29, 2015 microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015 The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015 The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014 1st. SAIBONICS symposium, Watanabe-kun got the best oral award.
Aug. 7, 2014 NIKKEI SANGYO SHIMBUN
210 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
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