WHAT's NEW
May. 13, 2020
A paper on a "De Novo Design of a Nanopore for DNA Detection Incorporating a β-hairpin Peptide" by Shimizu-kun is now preprinted in ChemRxiv!
Apr. 8, 2020
A paper on a "Competing Roles of Two Kinds of Ligand during Nonclassical Crystallization of Pillared-Layer Metal-Organic Frameworks Elucidated Using Microfluidic Systems" by Hiratani-san, Dr. Shoji, Dr. Kawano and Tanaka Lab. (Kwansei Gakuin Univ.) is now accepted in Chem. Eur. J.!
Apr. 1, 2020
Dr. Koiwai was assumed Assistant Professor!
Zheng Kaiyi has joined us as a research student!
Mar. 25, 2020
Master's degree (engineering) was awarded to Shimizu-kun, Takai-san and Tada-san, and bachelor's degree (engineering) was awarded to Usami-kun, Saito-san, Sato-kun and Takiguchi-kun.
Mar. 24, 2020
A paper on a "Dynamic behavior of an artificial protein needle contacting a membrane
observed by high-speed atomic force microscopy" by Takai-san, Dr. Kawano with Ueno Lab. (Tokyo tech.), Furuta Lab. (Tokyo tech.), Uchihashi Lab. (Nagoya University) is now accepted in Nanoscale!
Mar. 6, 2020
A paper about the study of nanopore probe conducted by Matsushita-kun, Takai-san
and Dr. Shoji became the Supplementary cover feature of J. Phys. Chem. B.!
Feb. 28, 2020
Usami-kun, Saitou-san, Satou-kun, Takiguchi-kun gave a presentation of Graduation thesis@TUAT.
Feb. 15-19, 2020
Takai-san gave an oral presentation and Shimizu-kun, Izumi-san and Yamaji-san gave a poster presentation on 64th Annual Meeting of the Biophysical Society@San Diego.
We visited Yamanaka Lab. @UC Riverside.
Previously update
EVENTS
Jun. 2 MicroTAS (abstract submission deadline)
Jun. 30 The 58th Annual meeting of the Biophysical
Society of Japan (abstract submission deadline)
Jul. 17 IRP (submission deadline)
Sep. 16-18 The 58th Annual meeting of
the Biophysical Society of Japan
RESEARCH
#1
#2
#3
#4
#5
#6
#7
#8
#9
#10
#11
#12
#13
#1
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure
under a low energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding
the nano scale structure produced by living things in artifacts.
#2
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress.
#3
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro electro
mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in lipid
bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
nano-space.
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques
in our study.
#4
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography with plasma-etching and exposure‑masking.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
#5
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
#6
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> DNA unzipping make the blocking time longer.
> Application: drug testing, food inspection, and diagnostic pathology.
#7
The mimetic device of cellular information transfer mechanism
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule level.
#8
Electrical molecular computing using nanopores
> Micro RNA(miRNA) is non-coding RNA, and it shows overexpress in cancer.
> The pattern recognition is necessary because expression pattern of miRNAs differ with
cancer types.
> This system can detect overexpress miRNAs in cancer specific by combination of DNA computing
and nanopore measurement.
> Application to simple diagnosis is expected.
#9
Characterization of various nanopores
> Nanopores used for sensing include various types of biological and solid nanopores.
> Characterization of these nanopores can find out optimal nanopore for sensing.
> By using nanopores with large diameter, it is possible to detect large molecules such as
diagnostic marker protein with high sensitivity.
#10
High-throughput measurement and preparation of droplets
> We achieved an artificial system generating two conductance states by embedding
a synthetic metal-organic porous (MOP) into lipid bilayers.
> Two-pore channels which are expressed ubiquitously in animals and plants control
the resting membrane potential by using dual channels.
> We demonstrated two distinct ion conductance states by embedding a single MOP
molecule with the geometry of an Archimedean cuboctahedron.
> The triangular and square apertures in the cuboctahedron work independently as
ion-transporting pathways.
#11
High-throughput measurement and preparation of droplets
> Antimicrobial peptides kill cell bacteria by forming nanopore into lipid bilayer.
> Pore-forming activity of the antimicrobial peptide is measured by electrical measurement.
> Current signals are classified by those of shapes, and we are enable to consider the
movement of peptides on membrane.
> This study contributes to clear the mechanism of antimicrobial peptides and to apply
them as antimicrobial drugs
#12
High-throughput measurement and preparation of droplets
> Molecular robots are composed of sensors, calculators, and actuators that are
all implemented in liposomes or hydrogels.
> Synthetic ion channels(nanopores) are potential candidates for the sensor sections
of molecular robots.
#13
High-throughput measurement and preparation of droplets
> The droplet contact method (DCM) has been reported to prepare stable planar bilayer
lipid membranes (pBLMs).
> In this study, we applied a DCM strategy to pBLM formation using microfluidic techniques
and attempted to form double-stacked pBLMs in micro-meter scale.
> First, microchannels with micro pillars were designed via hydrodynamic simulations to
form a five-layered flow with aqueous and lipid/oil solutions.
> Then, pBLMs were successfully formed by controlling the pumping pressure of the
solutions and allowing contact between the two lipid monolayers.
MEMBERS
Ryuji Kawano
Ph.D.
Associate Professor
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
Favorites
Climbing
Trail running
Kojiro (cat)
The doctor's course
Favorites : Sports
koiwai(@go.tuat.ac.jp)
・2016 - 2019: JSPS Research Fellowship (DC1)
・2019 - 2020: JSPS Research Fellowship (PD)
・2020 - : Assistant Professor, Toyko University of Agriculture and Technology
・2014: Bachelor's degree, Tokyo University of Marine Science and Technology
・2016: Master's of marine science,
Tokyo University of Marine Science and Technology
・2019: Ph. D., Tokyo University of Marine Science and Technology
liuping(@st.go.tuat.ac.jp)
Favorites : Eating
k-izumi(@st.go.tuat.ac.jp)
Favorites : Day trip, Running
n-takeuchi(@st.go.tuat.ac.jp)
Favorites : Drumming, Eating
y-numaguchi(@st.go.tuat.ac.jp)
Favorites : Watching figure skating
m-yamaji(@st.go.tuat.ac.jp)
Favorites : Watching baseball, Music
m-usami(@st.go.tuat.ac.jp)
Favorites : Play the guitar
c-saito(@st.go.tuat.ac.jp)
Favorites : Softball, Drinking
mi-sato(@st.go.tuat.ac.jp)
Favorites : Watching soccer, Yosakoi, Travel
s-takiguchi(@st.go.tuat.ac.jp)
Favorites : Basketball
Favorites : football, watching baseball
hayashi(@st.go.tuat.ac.jp)
favorite : baseball, drawing, travel
fukuda(@st.go.tuat.ac.jp)
favorite : playing the piano, walking
ikuro2mzgc(@st.go.tuat.ac.jp)
favorite : baseball, Music, travel
mitsuki(@st.go.tuat.ac.jp)
favorite : Watching TV-drama, travel
k-izumi(@st.go.tuat.ac.jp)
favorite : travel, running
kaiyi-zheng(@st.go.tuat.ac.jp)
Favorites：Watching anime & movies
Keiichiro Koiwai, Ph.D.
Liu Ping
Nanami takeuchi
Yuri Numaguchi
Misa Yamaji
Masataka Usami
Chihiro Saito
Mikiyoshi Sato
Sotaro Takiguchi
Aileen Cooney
Kohei Hayashi
Miyu Fukuda
Ikuro Mizoguchi
Mitsuki Miyagi
Zheng Kaiyi (Research student)
Undergraduates
Kayano Izumi
Assistant Professor
Keiko Okabe
Technical staff
Visiting Scholar
The master's course
PUBLICATIONS
Peer-reviewed Journals
- DNA origami nanoplate-based emulsion with designed nanopore function
Daisuke Ishikawa, Yuki Suzuki, Chikako Kurokawa, Masayuki Ohara, Misato Tsuchiya, Masamune Morita, Miho Yanagisawa,
Masayuki Endo, Ryuji Kawano, Masahiro Takinoue
Angew. Chem., Int. Ed. 2019 accepted. - Electrophysiological Analysis of Antimicrobial Peptides in Diverse Species
Naoki Saigo, Kayano Izumi, and Ryuji Kawano
ACS Omega, 2019 accepted. - Electrophysiological Analysis of Membrane Disruption by Bombinin and its Isomer using Lipid Bilayer System
Yusuke Sekiya, Keisuke Shimizu, Yuki Kitahashi, Akifumi Ohyama, Izuru Kawamura, and Ryuji Kawano
ACS Appl. Bio. Mater., Article ASAP, DOI:10.1021/acsabm.8b00835 [Link][pdf][press]
Peer-reviewed Journals
- Electrophysiological measurement of ion channels on plasma/organelle membranes using an on-chip lipid bilayer system
Koki Kamiya, Toshihisa Osaki, Kenji Nakao, Ryuji Kawano, Satoshi Fujii, Nobuo Misawa, Masatoshi Hayakawa, and Shoji Takeuchi
Scientific Reports, 2018, 17498, DOI:10.1038/s41598-018-35316-4[Link] - Nanopore Decoding of Oligonucleotides in DNA Computing
Ryuji Kawano
Biotechnology Journal, 2018, 1800091, DOI: 10.1002/biot.201800091[Link][pdf] - Spring-shaped stimuli-responsive hydrogel actuator with large deformation
Koki Yoshida, Shunsuke Nakajima, Ryuji Kawano, Hiroaki Onoe
Sensors and Actuators B-Chemical, 2018, 272, 361, DOI: 10.1016/j.snb.2018.05.174[Link] - Microfluidic Formation of Double-stacked Lipid Bilayers by Controlling the Water-Oil Interface
Kan Shoji and Ryuji Kawano
Micromachines, 2018, 9(5), 253, DOI: 10.3390/mi9050253[Link][pdf] - Channel Current Analysis Estimates the Pore-formation and the Penetration of Transmembrane Peptides
Yusuke Sekiya, Shungo Sakashita, Keisuke Shimizu, Kenji Usui and Ryuji Kawano
Analyst, 2018, 143(15), 3540-3543, DOI: 10.1039/C8AN00243F[Link][pdf][Cover] - DNA Logic Operation with Nanopore Decoding To Recognize MicroRNA Patterns in Small Cell Lung Cancer
Moe Hiratani and Ryuji Kawano
Analytical Chemistry, 2018, 90(14), 8531-8537, DOI: 10.1021/acs.analchem.8b01586[Link][pdf][Press1][Press2] - Design of protein-responsive micro-sized hydrogels for self-regulating microfluidic systems
Mayu Hirayama, Kazuhiro Tsuruta, Akifumi Kawamura, Masayuki Ohara, Kan Shoji, Ryuji Kawano and Takashi Miyata
Journal of Micromechanics and Microengineering, 2018, 28(3), DOI: 10.1088/1361-6439/aaa876[Link]
Peer-reviewed Journals
- Well-controlled cell-trapping systems for investigating heterogeneous cell-cell interactions
Koki Kamiya, Yuta Abe, Kosuke Inoue, Toshihisa Osaki, Ryuji Kawano, Norihisa Miki and Shoji Takeuchi
Advanced Healthcare Materials, 2018, 7(6), 2192-2659, DOI: 10.1002/adhm.201701208[Link][Cover] - Synthetic Ion Channels and DNA Logic Gates as Components of Molecular Robots (Invited Minireview)
Ryuji Kawano
ChemPhysChem, 2018, 19(4), 359-366, DOI: 10.1002/cphc.201700982[Link][PDF][Cover]
Peer-Reviewed Proceedings
- CONSTRUCTION OF PROGRAMMABLE NANOPORE USING β-SHEET PEPTIDES
Keisuke Shimizu, Naoki Saigo, Shungo Sakashita, Yoshio Hamada, Kenji Usui, Batsaikhan Mijiddorj, Izuru Kawamura and Ryuji Kawano
Proceedings of MicroTAS, 2018, 202-203. - SIMPLE FABRICATION OF A SOLID-STATE NANOPORE
Natsumi Takai, Masaki Matsushita, Kan Shoji, Tei Maki and Ryuji Kawano
Proceedings of MicroTAS, 2018, 570-571. - LIPOSOME DEFORMATION USING A TRANSMEMBRANE PEPTIDE
Kayano Izumi, Naoki Saigo, and Ryuji Kawano
Proceedings of MicroTAS, 2018, 1406-1408.
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Peer-reviewed Journals
- Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores
Hirokazu Watanabe, Alberto Gubbiotti, Mauro Chinappi, Natsumi Takai, Koji Tanaka, Kouhei Tsumoto and Ryuji Kawano
Analytical Chemistry, 2017, 89(21), 11269–11277, DOI: 10.1021/acs.analchem.7b01550[Link][pdf] - MicroRNA Detection at Femtomolar Concentration by a Biological Nanopore with an Isothermal Amplification
Haolin Zhang, Moe Hiratani, Kentaro Nagaoka and Ryuji Kawano
Nanoscale, 2017, 9, 16124-16127, DOI: 10.1039/C7NR04215A[Link][pdf][Press] - pH Regulates Pore Formation of a Protease Activated Vip3Aa from Bacillus thuringiensis
Thittaya Kunthic, Hirokazu Watanabe, Ryuji Kawano, Yoshikazu Tanaka, Boonhiang Promdonkoy, Min Yao and Panadda Boonserm
Biochimica et Biophysica Acta (BBA) - Biomembranes, 2017, 1859, 2234-2241, DOI: 10.1016/j.bbamem.2017.08.018[Link] - Serial DNA Relay in DNA Logic Gates by electrical Fusion and Mechanical Splitting of Droplets
iHiroki Yasuga, Kosuke Inoue, Ryuji Kawano, Masahiro Takinoue, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
PLOS ONE, 2017, 12(7), DOI: 10.1371/journal.pone.0180876[Link] - Nanopore Logic Operation with DNA to RNA Transcription in a Droplet System
Masayuki Ohara, Masahiro Takinoue and Ryuji Kawano
ACS Synthetic Biology, 2017, 6(7), 1427-1432, DOI: 10.1021/acssynbio.7b00101 [Link][pdf][Press] - Stimuli-responsive Hydrogel Microfibers with Controlled Anisotropic Shrinkage and Cross-sectional Geometries
Shunsuke Nakajima, Ryuji Kawano and Hiroaki Onoe
Soft Matter, 2017, 13, 3710-3719, DOI: 10.1039/C7SM00279C [Link] - Metal-Organic Cuboctahedra for Synthetic Ion Channels with Multiple Conductance States
Ryuji Kawano, Nao Horike, Yuh Hijikata, Mio Kondo, Arnau Carné-Sánchez, Patrick Larpent, Shuya Ikemura, Toshihisa Osaki, Koki Kamiya, Susumu Kitagawa, Shoji Takeuchi and Shuhei Furukawa
Chem, 2017, 2, 393-403, DOI: http://dx.doi.org/10.1016/j.chempr.2017.02.002 [Link] [pdf]
Peer-reviewed Journals
- Amplification and quantification of an antisense oligonucleotide from target microRNA using programmable DNA and a biological nanopore
Moe Hiratani, Masayuki Ohara and Ryuji Kawano
Analytical Chemistry, 2017, 89(4), 2312-2317, DOI: 10.1021/acs.analchem.6b03830 [Link] [pdf][Press] - Expression and characterization of the Plasmodium translocon of exported proteins component EXP2
Kazuaki Hakamada, Hirokazu Watanabe, Ryuji Kawano, Keiichi Noguchi and Masafumi Yohda
Biochemical and Biophysical Research Communications (BBRC), 2017, 482(4), 700-705, DOI:10.1016/j.bbrc.2016.11.097 [Link]
Peer-Reviewed Proceedings
- Lipobot: Negative Chemotaxis by Osmotic Pressure
K. Shoji and R. Kawano
Proceedings of MicroTAS, 2017, 963-964. - MicroRNA Pattern Recognition for Bile Duct Cancer Using Programmable DNA and Biological Nanopore
M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 219-220. - Precise Recognition for Translocation of Single-Stranded DNA Through a Biological Nanopore Using Wavelet Transform
A. Tamotsu, M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 1250-1251. - Nanopore Probe: Analysis of Hofmeister Effect in Nanospace
M. Matsushita and R. Kawano
Proceedings of MicroTAS, 2017, 281-282. - Design of Programmable Nanopore by Using β-sheet Peptides
K. Shimizu, N. Saigo, Y. Sekiya, S. Sakashita, Y. Hamada, K. Usui, and R. Kawano
Proceedings of MicroTAS, 2017, 1549-1550. - Biological Nanopore Filter: Separation and Detection of Oligonuceotides
A. Tada, A. Tamotsu, M. Hiratani and R. Kawano
Proceedings of MicroTAS, 2017, 1328-1329.
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Peer-reviewed Journals
- Rhodium–Organic Cuboctahedra as Porous Solids with Strong Binding Sites
Shuhei Furukawa, Nao Horike, Mio Kondo, Yuh Hijikata, Arnau Carné-Sánchez, Patrick Larpent, Nicolas Louvain, Stéphane Diring, Hiroshi Sato, Ryotaro Matsuda, Ryuji Kawano and Susumu Kitagawa
Inorganic Chemistry, 2016, 55 (21), 10843–10846, DOI: 10.1021/acs.inorgchem.6b02091 [Link] - Scalable fabrication of microneedle arrays via spatially controlled UV exposure
†Hidetoshi Takahashi, †Yun Jung Heo, Nobuchika Arakawa, Tesuo Kan, Kiyoshi Matsumoto, Ryuji Kawano and Isao Shimoyama (†equal contribution)
Microsystems & Nanoengineering, 2016, 2, 16049, DOI:10.1038/micronano.2016.49 [Link] - Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
Koki Kamiya, Ryuji Kawano, Toshihisa Osaki, Kazunari Akiyoshi and Shoji Takeuchi
Nature Chemistry, 2016, 8, 881–889, DOI:10.1038/nchem.2537 [Link] - Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array
Masayuki Ohara, Yusuke Sekiya and Ryuji Kawano
Electrochemistry, 2016, 84(5), 338-341, DOI: 10.5796/electrochemistry.84.338 [Link][pdf] - Logic Gate Operation by DNA Translocation through Biological Nanopores
†Hiroki Yasuga,† Ryuji Kawano, Masahiro Takinoue, Yutaro Tsuji, Toshihisa Osaki, Koki Kamiya, Norihisa Miki and Shoji Takeuchi
(†equal contribution)
PLoS One., 2016, 11(2):e0149667, DOI: 10.1371/journal.pone.0149667 [Link] - Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, using the Droplet Contact Method
Hirokazu Watanabe and Ryuji Kawano
Analytical Sciences, 2016, 32(1), 57-60, DOI: 10.2116/analsci.32.57 [Link][pdf]
Peer-Reviewed Proceedings
- A Single Molecular Logic Gate: “AND” Operation Using DNA Immobilized in Biological Nanopore
M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 118-119 - Recognition of microRNA Expression Pattern in Serum Using Programmable Droplet System for Cancer Diagnosis
M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1244-1245 - Direct Urinary Diagnosis System for Bladder Cancer: Fourier Analysis of microRNA Pattern From Nanopore Measurements
A. Tamotsu, M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 579-580 - Biological Nanopore Probe: Evaluation of Physicochemical Properties of DNA in Yocto (10-24) Litre Space
M. Matsushita, H. Watanabe, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1471-1472 - Exploer The Appropriate Biological Nanopore Selected from Pore Forming Protein Families
H. Watanabe, K. Tanaka, K. Tsumoto and R. Kawano
Proceedings of MicroTAS, 2016, 1304-1305 - A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine
Y. Sekiya, H. Watanabe, K. Usui and R. Kawano
Proceedings of MicroTAS, 2016, 595-596 - Molecular Evolution of Antimicrobial Peptides Using from Ascidiacea, Frog and Human
N. Saigo, Y. Sekiya, H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2016, 1511-1512
Peer-Reviewed Proceedings
- Double-Stacking Lipid Bilayer Formation Using Five-Layerd Microchannels
K. Shoji and R. Kawano
Proceedings of MicroTAS, 2016, 723-724 - Electrical Analysis of The Pore-Forming Translocon Using Lipid Bilayers in Droplet System
K. Hakamada, H. Watanabe, R. Kawano, K Noguchi and M. Yohda
Proceedings of MicroTAS, 2016, 659-660 - Self-Assembled Nanoplates at The Water-Oil Interface
D. Ishikawa, Y. Suzuki, C. Kurokawa, M. Ohara, M. Morita, M. Yanagisawa, R. Kawano, M. Endo and M. Takinoue
Proceedings of MicroTAS, 2016, 116-117 - Stimuli-Responsive Microfiber Fabricated with Double-Network Hydrogel
S. Nakajima, R. Kawano and H. Onoe
Proceedings of MicroTAS, 2016, 1128-1129
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Peer-reviewed Journals
- Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins
Y. Abe, K. Kamiya, T. Osaki, H. Sasaki, R. Kawano, N. Miki and S. Takeuchi
Analyst, 2015, 140, 5557-5562, DOI: 10.1039/C4AN02201G [Link] - Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications
M. Tani, R. Kawano, K. Kamiya & K. Okumura
Scientific Reports, 2015, 5, 10263, DOI: 10.1038/srep10263 [Link] - Preparation of Molecule-Responsive Microsized Hydrogels via Photopolymerization for Smart Microchannel Microvalves
Y. Shiraki, K. Tsuruta, J. Morimoto, C. Ohba, A. Kawamura, R. Yoshida, R. Kawano, T. Uragami and T. Miyata
Macromol. Rapid Commun., 2015, 36, 515–519, DOI: 10.1002/marc.201400676 [Link] - Preparation of Structurally Colored, Monodisperse Spherical Assemblies Composed of Black and White Colloidal
Particles using a Micro Flow-Focusing Device
M. Teshima, T. Seki, R. Kawano, S. Takeuchi, S. Yoshioka and Y. Takeoka
Journal of Materials Chemistry C, 2015, 3, 769 - 777, DOI: 10.1039/C4TC01929F. [Link]
Peer-Reviewed Proceedings
- Logic Gate Operation using Three-way Junction DNA and Biological Nanopore
M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 1493-1495 - EFFECTS OF ANTIMICROBIAL ACTIVITIES ON D- AND L-BOMBININ USING ARTIFICIAL BACTERIA CELL-MEMBRANE
Y. Sekiya, H. Watanabe, I. Kawamura, R.Kawano
Proceedings of MicroTAS, 2015, 823-825 - Autonomous Diagnosis and Therapy: miRNA Detection and Drug Release using Programmable DNA and Biological Nanopore
M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 906-908 - A SINGLE MOLECULE DETECTION OF PROTEIN BY APPROPRIATE SIZED BIOLOGICAL NANOPORES
H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2015, 51-53
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Peer-reviewed Journals
- A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
R. Kawano, Y. Tsuji, K. Kamiya, T. Kodama, T. Osaki, N. Miki, S. Takeuchi
PLoS ONE, 2014, 9(7): e102427, DOI: 10.1371/journal.pone.0102427 [Link] - Lipid bilayers on a picoliter microdroplet array for rapid fluorescence detection of membrane transport
T. Tonooka, K. Sato, T. Osaki, R. Kawano, S. Takeuchi
Small, 2014, 10, No. 16, 3275–3282, DOI: 10.1002/smll.201470093 [Link] (Selected as the cover of issue)
Peer-Reviewed Proceedings
- Extend The Size of Biological Nanopore Using Mgainin and Perforin Pores
H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2014, 2125-2127. - DNA/RNA Computing with Biological Nanopore in Droplets System: AND Operation Using RNA Polymerization
M. Ohara, M. Takinoue and R. Kawano
Proceedings of MicroTAS, 2014, 1790-1792.
Peer-reviewed Journals
- Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Anal. Chem., 2013, 85, 10913-10919. [Link] (†equal contribution) - Automated Parallel Recordings of Topologically Identified Single Ion Channels
R. Kawano, Y. Tsuji, K. Sato, T. Osaki, K. Kamiya, M. Hirano, T. Ide, N. Miki, S. Takeuchi
Scientific Reports, 2013, 3, 1995, DOI: 10.1038/srep01995. [Link] - Round-Tip Dielectrophoresis-Based Tweezers for Single Micro-Object Manipulation
T. Kodama, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi
Biosensors and Bioelectronics, 2013, 47, 206-212. [Link] - Droplet based lipid bilayer system integrated with microfluidic channels for solution exchange
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Lab on a Chip, 2013, 13, 1476-1481. [Link] (†equal contribution) (Selected as the inside cover of the issue)
2019
2018
2017
2016
2015
2014
2013
Cover Gallery
PAST
Proceedings, etc.
Jun. 14, 2019
A study about diagnostics of cancer with DNA computing was published in NIKKEI.
Apr. 24, 2019
A study about Bombinin was released from EurekAlert!
Apr. 16, 2019
A study about Bombinin was press released form TUAT and Yokohama National University.
Mar. 13, 2019
A study about Nanoneedle-Probe conducted by Dr. Shoji was published in Nikkei Sangyo Shinbun.
Feb. 22, 2019
A study about Nananeedle-Probe conducted by Shoji-san was press released from TUAT.
Oct. 10, 2018
A study about diagnostics of small cell lung cancer with DNA computing conducted by Hiratani-san was press released from EurekAlert!
Jul. 12, 2018
A study about diagnostics of small cell lung cancer with DNA computing conducted by Hiratani-san was press released from TUAT.
Oct. 13, 2017
A study about microRNA detection at femtomolar concentration conducted by Hiratani-san and Zhang-san was press released from TUAT.
Jun. 13, 2017
A study about synthetic ion channels conducted by Dr. Kawano was appeared in the Chem-Station.
May 17, 2017
The achievement of the study about nanopore DNA logic operation conducted by Ohara-san was published in Mynavi News.
May 15, 2017
The achievement of the study about nanopore DNA logic operation conducted by Ohara-san was press released from TUAT.
Mar. 3, 2017
The collaborating research with Prof. Furukawa (Kyoto Univ.) was published in Mynavi News.
Feb. 23, 2017
A study about theranostics was published in NIKKEI SANGYO SHIMBUN.
Feb. 11, 2017
A study about theranostics was published in Nikkei Web and Mynavi News.
Feb. 10, 2017
A study about theranostics conducted by Hiratani-san was press released from TUAT!
Oct. 19, 2016
Rapid detection of microRNA was published in NIKKEI SANGYO SHIMBUN.
Jan. 18, 2016
Measurement of membrane protein near the top of Mt. Fuji performed by Dr. Kawano was registered with Guinness World Records as "the highest altitude biological cell experiment took place at 3,623 m."
Aug. 7, 2014
Measurement of membrane protein near the top of Mt. Fuji was published in NIKKEI SANGYO SHIMBUN.
Feb 6, 2020
Oral presentation for Master theses, Shimizu-kun got the best presentation award.
Dec. 20-21, 2019
Liu-san, Sato-kun and Takiguchi-kun won a special award, Usami-kun and Takai-san won an audience award @Saibounics.
Nov. 19-21, 2019
Takai-san won excellent presentation award on CHEMINAS40@Shizuoka.
Oct. 23-25, 2019
Shimizu-kun won Award for Excellence at Poster Presentation on The 56th Japanese Peptide Symposium@Tokyo.
Sep. 24-26, 2019
Liu-san, Yamaji-san and Usami-kun won Travel Grant, and Shimizu-kun and Tada-san won Student Presentation Award on The 57th Annual Meeting of The Biophysical Society of Japan＠Miyazaki.
Mar. 14-15, 2019
Dr. Shoji, Takeuchi-san, Numaguchi-san won the Young Scientist Award on Molecular Robotics @ Tokyo Tech.
Dec. 19, 2018
5th Cyborgnics@Keio univ., Numaguchi-san won a special award and Takeuchi-san won an audience award.
Oct. 16, 2018
Dr. Kawano was commended by the Dean of TUAT!
Sep. 14-15, 2018
Dr. Kawano gave a lecture on The 28th Annual Meeting of The Japanese Society of Oral Medicine & The 31st Annual Meeting of The Japanese Society of Oral Diagnosis / Oral Medicine＠Yokohama and received a commendation.
Aug. 1, 2018
The 2nd science live ticket＠Konan Univ., Shimizu-kun and Izumi-san won encouragement award. Numaguchi-san won excellent presentation award.
Mar. 6, 2018
Molecular Robotics@Sendai, Shimizu-kun got the Young Researcher Poster Award.
Feb 21, 2018
Oral presentation for Graduation theses, Tada-san got the best presentation award.
Feb 6, 2018
Oral presentation for Master theses, Hiratani-san got the best presentation award.
Dec. 01, 2017
The 4th Cyborgnics symposium@TUAT, Saigo-kun and Tada-san won excellent dawn research award. Shimizu-kun won excellent presentation research award.
Sep. 25, 2017
The 34th Electrochemical Society of Japan Summer School, Hiratani-san got the best poster award.
Sep. 22, 2017
2nd Japan-Korea International Symposium on Cyborgnics@Beppu, Hiratani-san got the "BEST POSTER PRESENTATION AWARD."
Dec. 20, 2016
The 3rd Cyborgnics symposium@Suzukake Campus of Tokyo Tech., Matsushita-kun won excellent dawn research award.
Nov. 25-27, 2016
The 54th Annual Meeting of the Biophysical Society of Japan@Tsukuba International Congress Center, Hiratani-san won Student Presentation Award!
Oct. 24, 2016
Shoji-san won the 2016th research grant from The Okawa Foundation for Information and Telecommunications.
Oct. 9-14, 2016
microTAS 2016@Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS Young Researcher Poster Award.
Sep. 8-9, 2016
The 34th Electrochemical Society of Japan Summer School, Watanabe-kun got the best oral award and Hiratani-san got the best poster award.
Aug. 7-10, 2016
IEEE ICMA 2016@Harbin, Dr. Shoji got the "Toshio Fukuda Best Paper Award in Mechatronics."
Feb. 23, 2016
Oral presentation for Graduation theses of B4, Hiratani-san got the best presentation award.
Feb. 12, 2016
Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an alumni association.
2015. 12. 8
The 2nd Cyborgnics symposium@ Keio Univ., Sekiya-kun got the excellence research audience award. Hiratani-san got the
excellence research oral award.
Oct. 25 - 29, 2015
microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015
The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015
The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014
The 1st Cyborgnics symposium, Watanabe-kun got the best oral award.
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PRESS
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ADDRESS
205 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Email: rjkawano
at
cc.tuat.ac.jp
ACCESS
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
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