Jul. 24, 2017
Dr. Kawano was introduced in ACS Synthetic Biology the page of Introducing Our Authors.
Jul. 15, 2017
Hiratani-san, Tamotsu-kun, Shimizu-kun, Takai-san and Tada-san gave a poster presentation on Molecular robotics final symposium@ Tokyo.
Jul. 09, 2017
Shoji-san, Tamotsu-kun, Matsushita-kun and Tada-san were accepted as poster presenter (acceptance rate: 72%), and Hiratani-san was accepted as oral presenter (acceptance rate: 9.3%) in MicroTAS2017@Savannah.
Jun. 29, 2017
A paper about droplets DNA logic gate conducted by Yasuga-kun and Inoue-kun (Keio Univ.) was accepted in PLOS ONE. It was an achievement of the collaborative study with KISTEC, Keio Univ., Tokyo Tech.
Jun. 13, 2017
A study about artificial ionic channel was posted in Chem-Station that is a chemical portal site.
May 31, 2017
We joined softball tournament of life science and biotechnology department with Nakazawa lab.
May 22-23, 2017
Shoji-san, Saigou-kun, Tamotsu-kun, Matsushita-kun, Takai-san and Tada-san gave poster presentation on CHEMINAS 35@Tokyo tech.
May 15, 2017
The achievement of the study about nanopore DNA logic operation was press released from TUAT.
Apr. 17, 2017
The study about nanopore DNA logic operation conducted by Ohara-kun was accepted by ACS Synthetic Biology. It was the joint achievement with Takinoue laboratory of Tokyo Tech.
Apr. 10, 2017
The study about stimuli-responsive gel fiber with Onoe lab. was accepted by the Soft Matter.
Apr. 7, 2017
We held cherry blossom viewing with Yanagisawa lab.
Jul. 15, 2017 Molecular robotics final symposium@ Tokyo.
Sept. 1, 2017 Laboratory camp
Sept. 9, 2017 Analytical chemistry@ Tokyo
Sept. 10, 2017 Electrical chemistry@ Nagasaki
Sept. 19 - 21, 2017 Biophysical society@ Kumamoto
Sept. 22, 2017 Japan-Korea symposium@ Beppu
Sept. 25, 2017 Summer school of electrical chemistry@ Hachioji
Oct. 22 - 26, 2017 MicroTAS 2017@ U.S.
Nov. 17, 2017 Tenure-track symposium@ TUAT
Nov. 20 - 22, 2017 Peptide symposium@ Osaka
Dec. 1, 2017 Saibounics@ TUAT
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure under a low
energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding the nano
scale structure produced by living things in artifacts.
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro
electro mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in
lipid bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography in a clean room.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> Application: drug testing, food inspection, and diagnostic pathology.
The mimetic device of cellular information transfer mechanism
> Bottom-up construction with biological molecules for “Molecular robot”.
> Construction of logic circuits modeled after living cells by networking droplets.
> Large-scale parallel computation of the droplet system by combining chemical sensing
Electrical molecular computing using nanopores
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule
Reconstitution membrane proteins in lipid bilayers
> Antimicrobial peptides kill mycetocyte by forming nanopore into the cell membranes.
> Pore-forming of the antimicrobial peptide is measured electrically using artificial cell
> Clarification of pore-forming mechanism of the antimicrobial peptide and its application
of antimicrobial drugs are expected.
High-throughput measurement and preparation of droplets
J. Mater, Chem. C, 2015, 3, 769–777
Tani, M. et al., Sci. Rep., 2015, 5, 10263
> Microfluidic enables energy and sample saving in chemical analysis by microscale
operation or chemical reaction on a microchip.
> The flow velocity in micro channels can be controlled precisely.
> The simultaneous chemical reaction is caused by flowing into some reaction fields
> The control of a flux in an oil layer and a water layer enables to form the uniform-
Ryuji Kawano, Ph.D.
Associate Professor( tenure-track )
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
The master's course
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
Favorites : Chorus, Music, Mah‐jong
Favorites : Theatergoing, Savings
Favorites : American Football
Favorites : Electrical work
Favorites : Watching soccer, Aikido
Favorites : Soft Tennis
Favorites : Softball, Reading
Favorites : Cooking, Photo
Kan Shoji, Ph.D.
PRESS & AWARD
May 5, 2017 The achievement of the study about nanopore DNA logic operation was press released from TUAT.
Mar. 3, 2017 The collaborating research with Prof. Furukawa (Kyoto Univ.) was published in Mynavi News.
Feb. 10, 2017 Hiratani-san's study about theranostics was press released from TUAT!
Oct. 24, 2016 Shoji-san won the 2016th research grant from The Okawa Foundation for Information and
Oct. 19, 2016 The study about miRNA nanopore detection of Hiratani-san was appeared in the Nikkei Business Daily!
Oct. 9-14, 2016 microTAS 2016@ Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS
Young Researcher Poster Award.
Feb. 23, 2016 Oral presentation for Graduation theses of B4, Hiratani-san got the pest presentation award.
Feb. 12, 2016 Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an
Oct. 25 - 29, 2015 microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015 The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015 The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014 1st. SAIBONICS symposium, Watanabe-kun got the best oral award.
Aug. 7, 2014 NIKKEI SANGYO SHIMBUN
210 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
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