WHAT's NEW
Nov. 19-21, 2019
Takai-san and Sato-kun gave a poster presentation on CHEMINAS40@Shizuoka.
Takai-san won excellent presentation award!
Nov. 4-5, 2019
Dr. Kawano, Liu-san, Takeuchi-san and Numaguchi-san gave a lecture on Workshop on Microfluidic Lipid Bilayer System and Organ-on-a-chips@Univ. of Helsinki.
Ryuji Kawano “Artificial Cell-membrane System Constructed by Microfluidic Technologies”
Yuri Numaguchi “Electrophysiological Analysis of Aβ42 in Planar Lipid Bilayer Imitating Nervous Cell-Membrane”
Nanami Takeuchi “Nanopore decoding for microRNA pattern of cancer with DNA computation”
Ping Liu “Specific Detection of Point-Mutation-Position Using Biological Nanopore”
Nov. 1-2, 2019
Dr. Kawano gave a lecture on Imperial Seminer@Imperial College London.
"De Novo Design of Transmembrane Peptides Constructed in Artificial Cell Membrane System"
Oct. 29-31, 2019
Tada-san and Takiguchi-kun gave a poster presentation on ISNAC@Tokyo.
Oct. 27-31, 2019
Takeuchi-san and Numaguchi-san gave an oral presentation and Liu-san gave a poster presentation on MicroTAS2019@Basel.
Oct. 24-25, 2019
Dr. Kawano gave a lecture on DNA Nanotechnology Workshop@LJP, Paris.
"Nanopore decoding for DNA computation"
Oct. 23-25, 2019
Shimizu-kun, Izumi-san, Numaguchi-san, Usami-kun and Saito-san gave a poster presentation on The 56th Japanese Peptide Symposium@Tokyo. Shimizu-kun won Award for Excellence at Poster Presentation!
Oct. 17-18, 2019
Izumi-san and Takeuchi-san gave a poster presentation on Japanese Society for Cell Synthesis Research@Ehime.
Oct. 5, 2019
Dr. Kawano gave a lecture at 26th Peptide Forum@Tottori.
Previously update
EVENTS
Dec. 20-21 Saibounics
Jan. 8 The 1st International Symposium on Molecular
Jan. 9-10 Molecular Engine Area Meeting
Jan. 17-18 Liquid Biopsy
Feb. 15-19 64th Annual Meeting of the Biophysical Society
RESEARCH
#1
#2
#3
#4
#5
#6
#7
#8
#9
#10
#11
#12
#13
#1
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure
under a low energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding
the nano scale structure produced by living things in artifacts.
#2
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
#3
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro electro
mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in lipid bilayer
membranes.
> Application for biosensor, drug development and physicochemical study in controlled nano-space.
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in our study.
#4
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography with plasma-etching and exposure‑masking.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
#5
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
#6
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> DNA unzipping make the blocking time longer.
> Application: drug testing, food inspection, and diagnostic pathology.
#7
The mimetic device of cellular information transfer mechanism
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule level.
#8
Electrical molecular computing using nanopores
> Micro RNA(miRNA) is non-coding RNA, and it shows overexpress in cancer.
> The pattern recognition is necessary because expression pattern of miRNAs differ with cancer types.
> This system can detect overexpress miRNAs in cancer specific by combination of DNA computing
and nanopore measurement.
> Application to simple diagnosis is expected.
#9
Characterization of various nanopores
> Nanopores used for sensing include various types of biological and solid nanopores.
> Characterization of these nanopores can find out optimal nanopore for sensing.
> By using nanopores with large diameter, it is possible to detect large molecules such as
diagnostic marker protein with high sensitivity.
#10
High-throughput measurement and preparation of droplets
> We achieved an artificial system generating two conductance states by embedding
a synthetic metal-organic porous (MOP) into lipid bilayers.
> Two-pore channels which are expressed ubiquitously in animals and plants control
the resting membrane potential by using dual channels.
> We demonstrated two distinct ion conductance states by embedding a single MOP
molecule with the geometry of an Archimedean cuboctahedron.
> The triangular and square apertures in the cuboctahedron work independently as
ion-transporting pathways.
#11
High-throughput measurement and preparation of droplets
> Antimicrobial peptides kill cell bacteria by forming nanopore into lipid bilayer.
> Pore-forming activity of the antimicrobial peptide is measured by electrical measurement.
> Current signals are classified by those of shapes, and we are enable to consider the
movement of peptides on membrane.
> This study contributes to clear the mechanism of antimicrobial peptides and to apply
them as antimicrobial drugs
#12
High-throughput measurement and preparation of droplets
> Molecular robots are composed of sensors, calculators, and actuators that are
all implemented in liposomes or hydrogels.
> Synthetic ion channels(nanopores) are potential candidates for the sensor sections
of molecular robots.
#13
High-throughput measurement and preparation of droplets
> The droplet contact method (DCM) has been reported to prepare stable planar bilayer
lipid membranes (pBLMs).
> In this study, we applied a DCM strategy to pBLM formation using microfluidic techniques
and attempted to form double-stacked pBLMs in micro-meter scale.
>First, microchannels with micro pillars were designed via hydrodynamic simulations to
form a five-layered flow with aqueous and lipid/oil solutions.
>Then, pBLMs were successfully formed by controlling the pumping pressure of the
solutions and allowing contact between the two lipid monolayers.
MEMBERS
Ryuji Kawano, Ph.D.
Associate Professor
Research experience and Educational background
・2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
・2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
・2008: Postdoctral Fellow, University of Utah
・2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
・2005: Lecturer, VBL, Yokohama National Univsersity
・2005: Ph. D., Yokohama National University (Watanabe lab.)
・2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
Favorites
Bouldering
Kojiro (cat)
The master's course
kan-shoji(@m2.tuat.ac.jp)
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
kei-smz(@st.go.tuat.ac.jp)
Favorites : Soft Tennis
takai723(@st.go.tuat.ac.jp)
Favorites : Softball, Reading
ask-tada(@st.go.tuat.ac.jp)
Favorites : Cooking, Photo
liuping(@m2.tuat.ac.jp)
Favorites : Eating
k-izumi(@st.go.tuat.ac.jp)
Favorites : Day trip, Running
n-takeuchi(@st.go.tuat.ac.jp)
Favorites : Drumming, Eating
y-numaguchi(@st.go.tuat.ac.jp)
Favorites : Watching figure skating
m-yamaji(@st.go.tuat.ac.jp)
Favorites : Watching baseball, Music
m-usami(@st.go.tuat.ac.jp)
Favorites : Play the guitar
c-saito(@st.go.tuat.ac.jp)
Favorites : Softball, Drinking
mi-sato(@st.go.tuat.ac.jp)
Favorites : Watching soccer, Yosakoi, Travel
s-takiguchi(@st.go.tuat.ac.jp)
Favorites : Basketball
Favorites : Guitar, Karaoke
hayashi(@st.go.tuat.ac.jp)
favorite : baseball, drawing, travel
fukuda(@st.go.tuat.ac.jp)
favorite : playing the piano, walking
ikuro2mzgc(@st.go.tuat.ac.jp)
favorite : baseball, Music, travel
mitsuki(@st.go.tuat.ac.jp)
favorite : Watching TV-drama, travel
k-izumi(@st.go.tuat.ac.jp)
favorite : travel, running
Kan Shoji, Ph.D.
Keisuke Shimizu
Natsumi Takai
Asuka Tada
Liu Ping
Nanami takeuchi
Yuri Numaguchi
Misa Yamaji
Masataka Usami
Chihiro Saito
Mikiyoshi Sato
Sotaro Takiguchi
Iiro Kiiski
Kohei Hayashi
Miyu Fukuda
Ikuro Mizoguchi
Mitsuki Miyagi
Undergraduates
Kayano Izumi
Postdoctoral Fellow
Keiko Okabe
technical staff
Visiting Scholar
GIYSE program
Masaki Kamimura
PUBLICATIONS
Peer-reviewed Journals
Peer-reviewed Journals
Peer-reviewed Journals
Peer-Reviewed Proceedings
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Peer-reviewed Journals
Peer-Reviewed Proceedings
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Peer-reviewed Journals
Peer-Reviewed Proceedings
Peer-reviewed Journals
2019
2018
2017
2016
2015
2014
2013
Cover Gallery
PAST
Proceedings, etc.
Jun. 14, 2019
A study about diagnostics of cancer with DNA computing was published in NIKKEI.
Apr. 24, 2019
A study about Bombinin was released from EurekAlert!
Apr. 16, 2019
A study about Bombinin was press released form TUAT and Yokohama National University.
Mar. 13, 2019
A study about Nanoneedle-Probe conducted by Dr. Shoji was published in Nikkei Sangyo Shinbun.
Feb. 22, 2019
A study about Nananeedle-Probe conducted by Shoji-san was press released from TUAT.
Oct. 10, 2018
A study about diagnostics of small cell lung cancer with DNA computing conducted by Hiratani-san was press released from EurekAlert!
Jul. 12, 2018
A study about diagnostics of small cell lung cancer with DNA computing conducted by Hiratani-san was press released from TUAT.
Oct. 13, 2017
A study about microRNA detection at femtomolar concentration conducted by Hiratani-san and Zhang-san was press released from TUAT.
Jun. 13, 2017
A study about synthetic ion channels conducted by Dr. Kawano was appeared in the Chem-Station.
May 17, 2017
The achievement of the study about nanopore DNA logic operation conducted by Ohara-san was published in Mynavi News.
May 15, 2017
The achievement of the study about nanopore DNA logic operation conducted by Ohara-san was press released from TUAT.
Mar. 3, 2017
The collaborating research with Prof. Furukawa (Kyoto Univ.) was published in Mynavi News.
Feb. 23, 2017
A study about theranostics was published in NIKKEI SANGYO SHIMBUN.
Feb. 11, 2017
A study about theranostics was published in Nikkei Web and Mynavi News.
Feb. 10, 2017
A study about theranostics conducted by Hiratani-san was press released from TUAT!
Oct. 19, 2016
Rapid detection of microRNA was published in NIKKEI SANGYO SHIMBUN.
Jan. 18, 2016
Measurement of membrane protein near the top of Mt. Fuji performed by Dr. Kawano was registered with Guinness World Records as "the highest altitude biological cell experiment took place at 3,623 m."
Aug. 7, 2014
Measurement of membrane protein near the top of Mt. Fuji was published in NIKKEI SANGYO SHIMBUN.
Nov. 19-21, 2019
Takai-san won excellent presentation award on CHEMINAS40@Shizuoka.
Oct. 23-25, 2019
Shimizu-kun won Award for Excellence at Poster Presentation on The 56th Japanese Peptide Symposium@Tokyo.
Sep. 24-26, 2019
Liu-san, Yamaji-san and Usami-kun won Travel Grant, and Shimizu-kun and Tada-san won Student Presentation Award on The 57th Annual Meeting of The Biophysical Society of Japan@Miyazaki.
Mar. 14-15, 2019
Dr. Shoji, Takeuchi-san, Numaguchi-san won the Young Scientist Award on Molecular Robotics @ Tokyo Tech.
Dec. 19, 2018
5th Cyborgnics@Keio univ., Numaguchi-san won a special award and Takeuchi-san won an audience award.
Oct. 16, 2018
Dr. Kawano was commended by the Dean of TUAT!
Sep. 14-15, 2018
Dr. Kawano gave a lecture on The 28th Annual Meeting of The Japanese Society of Oral Medicine & The 31st Annual Meeting of The Japanese Society of Oral Diagnosis / Oral Medicine@Yokohama and received a commendation.
Aug. 1, 2018
The 2nd science live ticket@Konan Univ., Shimizu-kun and Izumi-san won encouragement award. Numaguchi-san won excellent presentation award.
Mar. 6, 2018
Molecular Robotics@Sendai, Shimizu-kun got the Young Researcher Poster Award.
Feb 21, 2018
Oral presentation for Graduation theses, Tada-san got the best presentation award.
Feb 6, 2018
Oral presentation for Master theses, Hiratani-san got the best presentation award.
Dec. 01, 2017
The 4th Cyborgnics symposium@TUAT, Saigo-kun and Tada-san won excellent dawn research award. Shimizu-kun won excellent presentation research award.
Sep. 25, 2017
The 34th Electrochemical Society of Japan Summer School, Hiratani-san got the best poster award.
Sep. 22, 2017
2nd Japan-Korea International Symposium on Cyborgnics@Beppu, Hiratani-san got the "BEST POSTER PRESENTATION AWARD."
Dec. 20, 2016
The 3rd Cyborgnics symposium@Suzukake Campus of Tokyo Tech., Matsushita-kun won excellent dawn research award.
Nov. 25-27, 2016
The 54th Annual Meeting of the Biophysical Society of Japan@Tsukuba International Congress Center, Hiratani-san won Student Presentation Award!
Oct. 24, 2016
Shoji-san won the 2016th research grant from The Okawa Foundation for Information and Telecommunications.
Oct. 9-14, 2016
microTAS 2016@Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS Young Researcher Poster Award.
Sep. 8-9, 2016
The 34th Electrochemical Society of Japan Summer School, Watanabe-kun got the best oral award and Hiratani-san got the best poster award.
Aug. 7-10, 2016
IEEE ICMA 2016@Harbin, Dr. Shoji got the "Toshio Fukuda Best Paper Award in Mechatronics."
Feb. 23, 2016
Oral presentation for Graduation theses of B4, Hiratani-san got the best presentation award.
Feb. 12, 2016
Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an alumni association.
2015. 12. 8
The 2nd Cyborgnics symposium@ Keio Univ., Sekiya-kun got the excellence research audience award. Hiratani-san got the
excellence research oral award.
Oct. 25 - 29, 2015
microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015
The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015
The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014
The 1st Cyborgnics symposium, Watanabe-kun got the best oral award.
V
V
PRESS
AWARD
ADDRESS
205 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Email: rjkawano
at
cc.tuat.ac.jp
ACCESS
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
LINKS
COPYRIGHT(c) 2014-2018 KAWANO Lab. All Rights Reserved.