WHAT's NEW
Jul. 24, 2017
Dr. Kawano was introduced in ACS Synthetic Biology the page of Introducing Our Authors.
Jul. 15, 2017
Hiratani-san, Tamotsu-kun, Shimizu-kun, Takai-san and Tada-san gave a poster presentation on Molecular robotics final symposium@ Tokyo.
Jul. 09, 2017
Shoji-san, Tamotsu-kun, Matsushita-kun and Tada-san were accepted as poster presenter (acceptance rate: 72%), and Hiratani-san was accepted as oral presenter (acceptance rate: 9.3%) in MicroTAS2017@Savannah.
Jun. 29, 2017
A paper about droplets DNA logic gate conducted by Yasuga-kun and Inoue-kun (Keio Univ.) was accepted in PLOS ONE. It was an achievement of the collaborative study with KISTEC, Keio Univ., Tokyo Tech.
Jun. 13, 2017
A study about artificial ionic channel was posted in Chem-Station that is a chemical portal site.
May 31, 2017
We joined softball tournament of life science and biotechnology department with Nakazawa lab.
May 22-23, 2017
Shoji-san, Saigou-kun, Tamotsu-kun, Matsushita-kun, Takai-san and Tada-san gave poster presentation on CHEMINAS 35@Tokyo tech.
May 15, 2017
The achievement of the study about nanopore DNA logic operation was press released from TUAT.
Apr. 17, 2017
The study about nanopore DNA logic operation conducted by Ohara-kun was accepted by ACS Synthetic Biology. It was the joint achievement with Takinoue laboratory of Tokyo Tech.
Apr. 10, 2017
The study about stimuli-responsive gel fiber with Onoe lab. was accepted by the Soft Matter.
Apr. 7, 2017
We held cherry blossom viewing with Yanagisawa lab.
Previously update
EVENTS
Jul. 15, 2017 Molecular robotics final symposium@ Tokyo.
Sept. 1, 2017 Laboratory camp
Sept. 9, 2017 Analytical chemistry@ Tokyo
Sept. 10, 2017 Electrical chemistry@ Nagasaki
Sept. 19 - 21, 2017 Biophysical society@ Kumamoto
Sept. 22, 2017 Japan-Korea symposium@ Beppu
Sept. 25, 2017 Summer school of electrical chemistry@ Hachioji
Oct. 22 - 26, 2017 MicroTAS 2017@ U.S.
Nov. 17, 2017 Tenure-track symposium@ TUAT
Nov. 20 - 22, 2017 Peptide symposium@ Osaka
Dec. 1, 2017 Saibounics@ TUAT
RESEARCH
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#10
#1
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure under a low
energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding the nano
scale structure produced by living things in artifacts.
#2
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
#3
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro
electro mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in
lipid bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
nano-space.
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in
our study.
#4
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography in a clean room.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
#5
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
#6
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> Application: drug testing, food inspection, and diagnostic pathology.
#7
The mimetic device of cellular information transfer mechanism
> Bottom-up construction with biological molecules for “Molecular robot”.
> Construction of logic circuits modeled after living cells by networking droplets.
> Large-scale parallel computation of the droplet system by combining chemical sensing
using nanopores.
#8
Electrical molecular computing using nanopores
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule
level.
#9
Reconstitution membrane proteins in lipid bilayers
> Antimicrobial peptides kill mycetocyte by forming nanopore into the cell membranes.
> Pore-forming of the antimicrobial peptide is measured electrically using artificial cell
membranes.
> Clarification of pore-forming mechanism of the antimicrobial peptide and its application
of antimicrobial drugs are expected.
#10
High-throughput measurement and preparation of droplets
J. Mater, Chem. C, 2015, 3, 769–777
Tani, M. et al., Sci. Rep., 2015, 5, 10263
> Microfluidic enables energy and sample saving in chemical analysis by microscale
operation or chemical reaction on a microchip.
> The flow velocity in micro channels can be controlled precisely.
> The simultaneous chemical reaction is caused by flowing into some reaction fields
thorough microchannels.
> The control of a flux in an oil layer and a water layer enables to form the uniform-
sized microdroplet.
MEMBERS
Ryuji Kawano, Ph.D.
Associate Professor( tenure-track )
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
Favorites
Bouldering
Kojiro (cat)
The master's course
kan-shoji(@m2.tuat.ac.jp)
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
Favorites : Chorus, Music, Mah‐jong
s145903x(@st.go.tuat.ac.jp)
Favorites : Theatergoing, Savings
s122806v(@st.go.tuat.ac.jp)
s137112r(@st.go.tuat.ac.jp)
Favorites : American Football
s138243r(@st.go.tuat.ac.jp)
Favorites : Electrical work
s137664x(@st.go.tuat.ac.jp)
Favorites : Watching soccer, Aikido
kei-smz@st.go.tuat.ac.jp
Favorites : Soft Tennis
takai723@st.go.tuat.ac.jp
Favorites : Softball, Reading
ask-tada@st.go.tuat.ac.jp
Favorites : Cooking, Photo
Kan Shoji, Ph.D.
Yusuke Sekiya
Moe Hiratani
Naoki Saigo
Akihiro Tamotsu
Masaki Matsushita
Keisuke Shimizu
Natsumi Takai
Asuka Tada
Liu Ping
Undergraduates
Postdoctoral Fellow
Research student
PUBLICATIONS
- Serial DNA Relay in DNA Logic Gates by electrical Fusion and Mechanical Splitting of Droplets
H. Yasuga, K. Inoue, R. Kawano, M. Takinoue, T.Osaki, K. Kamiya, N. Miki, S. Takeuchi
PLOS ONE, accepted. - Nanopore Logic Operation with DNA to RNA Transcription in a Droplet System
M. Ohara, M. Takinoue, R. Kawano
ACS Synthetic Biology, Article ASAP, DOI: 10.1021/acssynbio.7b00101 [Link] - Stimuli-responsive Hydrogel Microfibers with Controlled Anisotropic Shrinkage and Cross-sectional Geometries
Shunsuke Nakajima, Ryuji Kawano, Hiroaki Onoe
Soft Matter, 2017, Advance Article, DOI: 10.1039/C7SM00279C [Link] - Metal-Organic Cuboctahedra for Synthetic Ion Channels with Multiple Conductance States
Ryuji Kawano, Nao Horike, Yuh Hijikata, Mio Kondo, Arnau Carné-Sánchez, Patrick Larpent, Shuya Ikemura, Toshihisa Osaki, Koki Kamiya, Susumu Kitagawa, Shoji Takeuchi and Shuhei Furukawa
Chem, 2017, 2, 393-403, DOI: http://dx.doi.org/10.1016/j.chempr.2017.02.002 [Link] - Amplification and quantification of an antisense oligonucleotide from target microRNA using programmable DNA and a biological nanopore
M. Hiratani, M. Ohara and R. Kawano
Analytical Chemistry, 2017, 89(4), 2312-2317, DOI: 10.1021/acs.analchem.6b03830 [Link] - Expression and characterization of the Plasmodium translocon of exported proteins component EXP2
K. Hakamada, H. Watanabe, R. Kawano, K. Noguchi, M. Yohda
Biochemical and Biophysical Research Communications (BBRC), 2017, 482(4), 700-705, DOI:10.1016/j.bbrc.2016.11.097 [Link]
Peer-reviewed Journals
- Rhodium–Organic Cuboctahedra as Porous Solids with Strong Binding Sites
S. Furukawa, N. Horike, M. Kondo, Y. Hijikata, A. Carné-Sánchez, P. Larpent, N. Louvain, S. Diring, H. Sato, R. Matsuda, R. Kawano,
S. Kitagawa
Inorg. Chem., 2016, 55 (21), 10843–10846, DOI: 10.1021/acs.inorgchem.6b02091 [Link] - Scalable fabrication of microneedle arrays via spatially controlled UV exposure
†H. Takahashi, †Y.J. Heo, N. Arakawa, T. Kan, K. Matsumoto, R. Kawano, I. Shimoyama (†equal contribution)
Microsystems & Nanoengineering, 2016, 2, 16049, DOI:10.1038/micronano.2016.49 [Link] - Cell-sized asymmetric lipid vesicles facilitate the investigation of asymmetric membranes
K. Kamiya, R. Kawano, T. Osaki, K. Akiyoshi, S. Takeuchi
Nature Chemistry, 2016, 8, 881-889, DOI:10.1038/nchem.2537 [Link] - Hairpin DNA Unzipping Analysis Using a Biological Nanopore Array
M. Ohara, Y. Sekiya, R. Kawano
Electrochemistry, 2016, 84(5), 338-341, DOI: 10.5796/electrochemistry.84.338 [Link] - Logic Gate Operation by DNA Translocation through Biological Nanopores
†H. Yasuga,† R. Kawano, M. Takinoue, T. Tsuji, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi (†equal contribution)
PLoS One. 2016, 11(2):e0149667, DOI: 10.1371/journal.pone.0149667 [Link] - Channel Current Analysis for Pore-forming Properties of an Antimicrobial Peptide, Magainin 1, using the Droplet Contact Method
H. Watanabe and R. Kawano
Analytical Sciences, 2016, 32(1), 57-60, DOI: 10.2116/analsci.32.57 [Link][pdf]
Peer-Reviewed Proceedings
- A Single Molecular Logic Gate: “AND” Operation Using DNA Immobilized in Biological Nanopore
M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 118-119 - Recognition of microRNA Expression Pattern in Serum Using Programmable Droplet System for Cancer Diagnosis
M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1244-1245 - Direct Urinary Diagnosis System for Bladder Cancer: Fourier Analysis of microRNA Pattern From Nanopore Measurements
A. Tamotsu, M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 579-580 - Biological Nanopore Probe: Evaluation of Physicochemical Properties of DNA in Yocto (10-24) Litre Space
M. Matsushita, H. Watanabe, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2016, 1471-1472 - Exploer The Appropriate Biological Nanopore Selected from Pore Forming Protein Families
H. Watanabe, K. Tanaka, K. Tsumoto and R. Kawano
Proceedings of MicroTAS, 2016, 1304-1305 - A Systematic Study on Molecular Mechanism of Pore-Forming Peptides for Discovering Antimicrobial Medicine
Y. Sekiya, H. Watanabe, K. Usui and R. Kawano
Proceedings of MicroTAS, 2016, 595-596 - Molecular Evolution of Antimicrobial Peptides Using from Ascidiacea, Frog and Human
N. Saigo, Y. Sekiya, H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2016, 1511-1512
- Double-Stacking Lipid Bilayer Formation Using Five-Layerd Microchannels
K. Shoji and R. Kawano
Proceedings of MicroTAS, 2016, 723-724 - Electrical Analysis of The Pore-Forming Translocon Using Lipid Bilayers in Droplet System
K. Hakamada, H. Watanabe, R. Kawano, K Noguchi and M. Yohda
Proceedings of MicroTAS, 2016, 659-660 - Self-Assembled Nanoplates at The Water-Oil Interface
D. Ishikawa, Y. Suzuki, C. Kurokawa, M. Ohara, M. Morita, M. Yanagisawa, R. Kawano, M. Endo and M. Takinoue
Proceedings of MicroTAS, 2016, 116-117 - Stimuli-Responsive Microfiber Fabricated with Double-Network Hydrogel
S. Nakajima, R. Kawano and H. Onoe
Proceedings of MicroTAS, 2016, 1128-1129
^
v
Peer-reviewed Journals
- Nonlinear concentration gradients regulated by the width of channels for observation of half maximal inhibitory concentration (IC50) of transporter proteins
Y. Abe, K. Kamiya, T. Osaki, H. Sasaki, R. Kawano, N. Miki and S. Takeuchi
Analyst, 2015, 140, 5557-5562, DOI: 10.1039/C4AN02201G [Link] - Towards combinatorial mixing devices without any pumps by open-capillary channels: fundamentals and applications
M. Tani, R. Kawano, K. Kamiya & K. Okumura
Scientific Reports, 2015, 5, 10263, DOI: 10.1038/srep10263 [Link] - Preparation of Molecule-Responsive Microsized Hydrogels via Photopolymerization for Smart Microchannel Microvalves
Y. Shiraki, K. Tsuruta, J. Morimoto, C. Ohba, A. Kawamura, R. Yoshida, R. Kawano, T. Uragami and T. Miyata
Macromol. Rapid Commun., 2015, 36, 515–519, DOI: 10.1002/marc.201400676 [Link] - Preparation of Structurally Colored, Monodisperse Spherical Assemblies Composed of Black and White Colloidal
Particles using a Micro Flow-Focusing Device
M. Teshima, T. Seki, R. Kawano, S. Takeuchi, S. Yoshioka and Y. Takeoka
Journal of Materials Chemistry C, 2015, 3, 769 - 777, DOI: 10.1039/C4TC01929F. [Link]
Peer-Reviewed Proceedings
- Logic Gate Operation using Three-way Junction DNA and Biological Nanopore
M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 1493-1495 - EFFECTS OF ANTIMICROBIAL ACTIVITIES ON D- AND L-BOMBININ USING ARTIFICIAL BACTERIA CELL-MEMBRANE
Y. Sekiya, H. Watanabe, I. Kawamura, R.Kawano
Proceedings of MicroTAS, 2015, 823-825 - Autonomous Diagnosis and Therapy: miRNA Detection and Drug Release using Programmable DNA and Biological Nanopore
M. Hiratani, M. Ohara and R. Kawano
Proceedings of MicroTAS, 2015, 906-908 - A SINGLE MOLECULE DETECTION OF PROTEIN BY APPROPRIATE SIZED BIOLOGICAL NANOPORES
H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2015, 51-53
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v
Peer-reviewed Journals
- A Portable Lipid Bilayer System for Environmental Sensing with a Transmembrane Protein
R. Kawano, Y. Tsuji, K. Kamiya, T. Kodama, T. Osaki, N. Miki, S. Takeuchi
PLoS ONE, 2014, 9(7): e102427, DOI: 10.1371/journal.pone.0102427 [Link] - Lipid bilayers on a picoliter microdroplet array for rapid fluorescence detection of membrane transport
T. Tonooka, K. Sato, T. Osaki, R. Kawano, S. Takeuchi
Small, 2014, 10, No. 16, 3275–3282, DOI: 10.1002/smll.201470093 [Link] (Selected as the cover of issue)
Peer-Reviewed Proceedings
- Extend The Size of Biological Nanopore Using Mgainin and Perforin Pores
H. Watanabe and R. Kawano
Proceedings of MicroTAS, 2014, 2125-2127. - DNA/RNA Computing with Biological Nanopore in Droplets System: AND Operation Using RNA Polymerization
M. Ohara, M. Takinoue and R. Kawano
Proceedings of MicroTAS, 2014, 1790-1792.
Peer-reviewed Journals
- Droplet Split-and-Contact Method for High-Throughput Transmembrane Electrical Recording
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Anal. Chem., 2013, 85, 10913-10919. [Link] (†equal contribution) - Automated Parallel Recordings of Topologically Identified Single Ion Channels
R. Kawano, Y. Tsuji, K. Sato, T. Osaki, K. Kamiya, M. Hirano, T. Ide, N. Miki, S. Takeuchi
Scientific Reports, 2013, 3, 1995, DOI: 10.1038/srep01995. [Link] - Round-Tip Dielectrophoresis-Based Tweezers for Single Micro-Object Manipulation
T. Kodama, T. Osaki, R. Kawano, K. Kamiya, N. Miki, S. Takeuchi
Biosensors and Bioelectronics, 2013, 47, 206-212. [Link] - Droplet based lipid bilayer system integrated with microfluidic channels for solution exchange
†Y. Tsuji,†R. Kawano, T. Osaki, K. Kamiya, N. Miki, S. Takeuchi
Lab on a Chip, 2013, 13, 1476-1481. [Link] (†equal contribution) (Selected as the inside cover of the issue)
2017
2016
2015
2014
2013
PRESS & AWARD
May 5, 2017 The achievement of the study about nanopore DNA logic operation was press released from TUAT.
Mar. 3, 2017 The collaborating research with Prof. Furukawa (Kyoto Univ.) was published in Mynavi News.
Feb. 10, 2017 Hiratani-san's study about theranostics was press released from TUAT!
Oct. 24, 2016 Shoji-san won the 2016th research grant from The Okawa Foundation for Information and
Telecommunications.
Oct. 19, 2016 The study about miRNA nanopore detection of Hiratani-san was appeared in the Nikkei Business Daily!
Oct. 9-14, 2016 microTAS 2016@ Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS
Young Researcher Poster Award.
Feb. 23, 2016 Oral presentation for Graduation theses of B4, Hiratani-san got the pest presentation award.
Feb. 12, 2016 Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an
alumni association.
Oct. 25 - 29, 2015 microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015 The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015 The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014 1st. SAIBONICS symposium, Watanabe-kun got the best oral award.
Aug. 7, 2014 NIKKEI SANGYO SHIMBUN
ADDRESS
210 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Email: rjkawano
at
cc.tuat.ac.jp
ACCESS
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
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