Jun. 28, 2016
The paper about "micro-needles" collaborated with Prof. Heo (TUAT) and Prof. Shimoyama (U of Tokyo) is accepted in Microsystems & Nanoengineering (Nature Publishing Group)!
Jun. 25, 2016
All lab members are accepted in MicroTAS2016. Ohara-kun will give a oral presentation (acceptance ratio=9%) in there!
Jun. 14, 2016
The paper about "asymmetric vesicles" collaborated with Prof. Takeuchi group (KAST) is accepted in Nature Chemistry!
Jun. 1, 2016
Ms. Miyazawa joined us as a technical stuff.
Apr. 25-26, 2016
Four students gave a poster presentation on the 33rd CHEMINAS@U of Tokyo.
Apr. 1, 2016
Dr. Shoji-san joined us as a post doctorial fellow.
Mar. 14-16, 2016
Dr. Kawano gave an oral presentation, and Ohara-kun, Watanabe-kun, Sekiya-kun and Hiratani-san gave a poster presentation on the 7th Meeting of Molecular Robotics @Gamagori.
Mar. 6-10, 2016
Dr. Kawano gave a talk on PITTCON 2016 @Atlanta, USA.
Feb. 5, 2016
The paper about DNA unzipping by Ohara and Sekiya was accepted in Electrochemistry!
Feb. 4, 2016
The paper about DNA logic gate (NAND) by Yasuga (KAST), Prof. Takeuchi (UT), and Prof. Takinoue (Tokyo Tech.) was accepted in PLOS ONE!
Jan. 18 2016
Dr. Kawano's application for "Highest altitude biological cell experiment" has been accepted at the Guinness World Records!
He is now the Guinness World Records Title Holder!
Oct. 2-7, 2016
Dr. Kawano, Dr. Shoji, and Mr. Watanabe will give the presentation in PRiME2016@Hawaii, USA.
Oct. 9-13, 2016
All lab members will give the presentation in MiroTAS2016@Dublin, Ireland.
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure under a low
energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding the nano
scale structure produced by living things in artifacts.
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro
electro mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in
lipid bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography in a clean room.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> Application: drug testing, food inspection, and diagnostic pathology.
The mimetic device of cellular information transfer mechanism
> Bottom-up construction with biological molecules for “Molecular robot”.
> Construction of logic circuits modeled after living cells by networking droplets.
> Large-scale parallel computation of the droplet system by combining chemical sensing
Electrical molecular computing using nanopores
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule
Reconstitution membrane proteins in lipid bilayers
> Antimicrobial peptides kill mycetocyte by forming nanopore into the cell membranes.
> Pore-forming of the antimicrobial peptide is measured electrically using artificial cell
> Clarification of pore-forming mechanism of the antimicrobial peptide and its application
of antimicrobial drugs are expected.
High-throughput measurement and preparation of droplets
J. Mater, Chem. C, 2015, 3, 769–777
Tani, M. et al., Sci. Rep., 2015, 5, 10263
> Microfluidic enables energy and sample saving in chemical analysis by microscale
operation or chemical reaction on a microchip.
> The flow velocity in micro channels can be controlled precisely.
> The simultaneous chemical reaction is caused by flowing into some reaction fields
> The control of a flux in an oil layer and a water layer enables to form the uniform-
Ryuji Kawano, Ph.D.
Associate Professor( tenure-track )
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
The master's course
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
Favorites : Photo, PC, Swimming
Favorites : Travel, Jogging, Eating
Favorites : Chorus, Music, Mah‐jong
Favorites : Theatergoing, Savings
Kan Shoji, Ph.D.
PRESS & AWORD
Feb. 23, 2016 Oral presentation for Graduation theses of B4, Hiratani-san got the pest presentation award.
Feb. 12, 2016 Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an
Oct. 25 - 29, 2015 microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015 The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015 The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014 1st. SAIBONICS symposium, Watanabe-kun got the best oral award.
Aug. 7, 2014 NIKKEI SANGYO SHIMBUN
210 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
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