Nov. 30, 2016
Kawano gave a talk on TAMA Association's Medical Innovation Forum @Hachiohji.
Nov. 25-27, 2016
The 54th Annual Meeting of the Biophysical Society of Japan ＠Tsukuba International Congress Center. All M1 and B4 member gave poster presentation. Hiratani-san won Student Presentation Award!
Nov. 16, 2016
A paper about the study of translocon with Yohda laboratory was accepted in Biochemical and Biophysical Research Communications (BBRC).
Nov. 14, 2016
Hiratani-san gave a poster presentation on The 6th CSJ Chemistry Festa@Tower Hall Funabori.
Nov. 9, 2016
We participated in the Biotechnology and Life Science softball tournament with Nakazawa laboratory.
Nov. 8, 2016
Dr. Kawano gave a talk on MNC2016 (29th International Microprocesses and Nanotechnology Conference)@Kyoto.
Nov. 7, 2016
Dr. Kawano gave a talk at Kyoto University Institute for Chemical Research.
Oct. 26-28, 2016
Watanabe-kun, Sekiya-kun and Saigo-kun gave a poster presentation on The 53rd Japanese Peptide Symposium@Kyoto Terrsa.
Oct. 25-27, 2016
Shoji-san gave a poster presentation on Chem-Bio Informatics Society Annual Meeting 2016@Tower Hall Funabori.
Oct. 24, 2016
Shoji-san won the 2016th research grant from The Okawa Foundation for Information and Telecommunications.
Artificial objects and Natural products
> In the nature, molecules assemble by themselves and produce a minute nanostructure under a low
energy (low temperature, ordinary pressure).
> Microfabrication can create controlled microstructures.
> It is difficult to create natural structures under a nanoscale (missing area).
> We construct a structure controlled sizes from an angstrom to a meter scale, embedding the nano
scale structure produced by living things in artifacts.
Artificial cell membranes (Lipid Bilayer) and membrane proteins
> Phospholipid molecules spontaneously form bilayer lipid membranes by
Hydrophobic and Hydrophilic Interaction in the water.
> Membrane proteins perform various functions in the cell membranes.
> Some membrane protein transport substances, energy and information between
outside and inside the cells.
> Membrane proteins perform as the transporter of ions and medicines, the receptor
of odorant and light, and the perception of mechanical stress
MEMS(MicroElectroMechanicalSystem) and membrane proteins
In our laboratory,
> We manufacture the platform for preparing artificial cell membranes by using a micro
electro mechanical system (MEMS).
> System construction for measuring the signals of membrane protein reconstituted in
lipid bilayer membranes.
> Application for biosensor, drug development and physicochemical study in controlled
> Utilizing molecules, proteins, DNA/RNA, lipid, cell, MEMS, microfluidics techniques in
Micro-sized processing techniques (Lithography, Milling)
> Microfabrication by photolithography in a clean room.
> CAD/CAM micromilling for plastic fabrication.
> Manufacture of electrodes by metal deposition and confirmation of processing accuracy
by a measuring microscope.
Droplet contact method: Formation of artificial cell membranes
> Applying of oil/lipid solution and buffer solution into a chamber, two lipid monolayers
are formed at the surface of aqueous droplets.
> Two droplet contact together and bilayer lipid membrane can be formed.
> Artificial cell membranes mimicking natural cell membranes are able to be formed by
various types of lipid molecules.
A electrical single molecule detection by nanopore sensing
> Ions pass through a nanopore (≈1.4 nm in diameter) reconstituted in membranes
cause current increase.
> A translocating molecules through the pore show the blocking current.
> Application: drug testing, food inspection, and diagnostic pathology.
The mimetic device of cellular information transfer mechanism
> Bottom-up construction with biological molecules for “Molecular robot”.
> Construction of logic circuits modeled after living cells by networking droplets.
> Large-scale parallel computation of the droplet system by combining chemical sensing
Electrical molecular computing using nanopores
> DNA computing, which uses programmed DNA as logic elements, enables large-scale
parallel computation which is difficult for existing computers.
> Although this calculation requires longer detection time because of DNA amplification
by PCR and detection by fluorescence imaging, we implemented a reduction of detection
time by electrical detection using nanopore sensing.
> Nanopores enable the detection and translocation to next droplet at the single molecule
Reconstitution membrane proteins in lipid bilayers
> Antimicrobial peptides kill mycetocyte by forming nanopore into the cell membranes.
> Pore-forming of the antimicrobial peptide is measured electrically using artificial cell
> Clarification of pore-forming mechanism of the antimicrobial peptide and its application
of antimicrobial drugs are expected.
High-throughput measurement and preparation of droplets
J. Mater, Chem. C, 2015, 3, 769–777
Tani, M. et al., Sci. Rep., 2015, 5, 10263
> Microfluidic enables energy and sample saving in chemical analysis by microscale
operation or chemical reaction on a microchip.
> The flow velocity in micro channels can be controlled precisely.
> The simultaneous chemical reaction is caused by flowing into some reaction fields
> The control of a flux in an oil layer and a water layer enables to form the uniform-
Ryuji Kawano, Ph.D.
Associate Professor( tenure-track )
Research experience and Educational background
･2014.1-present: Associate Professor, Tokyo University of Agriculture and Technology
･2009: Researcher, Kanagawa Academy of Science and Technology (Takeuchi lab.)
･2008: Postdoctral Fellow, University of Utah
･2006: JSPS Postdoctroal Fellow, University of Utah (Henry. S. White lab.)
･2005: Lecturer, VBL, Yokohama National Univsersity
･2005: Ph. D., Yokohama National University (Watanabe lab.)
･2000: B. Eng., Tokyo Metropolitan University (Iyoda lab.)
The master's course
Favorites :Playing baseball
・2016: JSPS Research Fellowship (PD)
・2014: JSPS Research Fellowship (DC2)
・2016: Ph. D., Osaka University
・2013: Master of Engineering, Tokyo University of Agriculture and Technology
・2011: Bachelor’s degree, Tokyo University of Agriculture and Technology
Favorites : Photo, PC, Swimming
Favorites : Travel, Jogging, Eating
Favorites : Chorus, Music, Mah‐jong
Favorites : Theatergoing, Savings
Kan Shoji, Ph.D.
PRESS & AWARD
Oct. 24, 2016 Shoji-san won the 2016th research grant from The Okawa Foundation for Information and
Oct. 19, 2016 The study about miRNA nanopore detection of Hiratani-san was appeared in the Nikkei Business Daily!
Oct. 9-14, 2016 microTAS 2016@ Dublin, Ireland, Ohara-kun gave oral presentation. Hiratani-san won CHEMINAS
Young Researcher Poster Award.
Feb. 23, 2016 Oral presentation for Graduation theses of B4, Hiratani-san got the pest presentation award.
Feb. 12, 2016 Ohara-kun, Watanabe-kun, Sekiya-kun, Hiratani-san got the research encouragement award for an
Oct. 25 - 29, 2015 microTAS 2015@ Gyeongju, Korea Watanabe-kun gave oral presentation.
Oct. 13 - 15, 2015 The 5th CSJ chemical festa 2015, Watanabe-kun got the best poster award.
Aug. 26 - 27, 2015 The 33th Electrochemical Society of Japan Summer School, Watanabe-kun got the best poster award.
Dec. 19, 2014 1st. SAIBONICS symposium, Watanabe-kun got the best oral award.
Aug. 7, 2014 NIKKEI SANGYO SHIMBUN
210 Building 12, Tokyo University of Agriculture and Technology
2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588
Take the JR Chuo Line from Tokyo Station (rapid train) to Higashi-Koganei Station: 40 minutes.
Walk about 10 minutes to campus.
Take the JR Chuo line tom Musashi-Koganei Station.
Walk about 20 minutes to campus.
・Tokyo University of Agriculture and Technology
・Tokyo University of Agriculture and Technology/ Biotechnology and Life Science
・Tokyo University of Agriculture and Technology/ tenure-track program
・Kyoto University/ KyotoInstitute for Chemical Research
・The University of Tokyo / TAKEUCHI RESEARCH GROUP
・Tokyo Institute of Technology / Takinoue Lab.
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