論文・実績

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2018年

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Shiina, T., Nakagawa, K., Fujisaki, Y., Ozaki, T., Toyomasu, T., Hashimoto, M., Koshino, H., Minami A., Kawaide, H. & Oikawa, H.
Biosynthetic study of conidiation-inducing factor conidiogenone: Heterologous production and cyclization mechanism of a key bifunctional diterpene synthase Biosci.Biotechnol. Biochem
論文リンク

Takuya Hasegawa, Atsushi Okabe, Yusuke Kato, Atsushi Ooshiro, Hiroshi Kawaide and Masahiro Natsume
Ethyl β-d-glucoside: a novel chemoattractant of Ralstonia solanacearum isolated from tomato root exudates by a bioassay-guided fractionation Bioscience, Biotechnology, and Biochemistry
論文リンク

Hajime Hayakawa, Kento Motoyama, Fumiaki Sobue, Tomokazu Ito, Hiroshi Kawaide, Tohru Yoshimura, and Hisashi Hemmi
Modified mevalonate pathway of the archaeon Aeropyrum pernix proceeds via trans-anhydromevalonate 5-phosphate Proc. Natl. Acad. Sci. USA(2018) doi/10.1073/pnas.1809154115
論文リンク

Miyazaki S., Hara M., Ito S., Tanaka K., Asami T., Hayashi K.-i., Kawaide H., and Nakajima M.
An ancestral gibberellin in a moss Physcomitrella patens Molecular Plantdoi:10.1016/j.molp.2018.03.010.
論文リンク

2017年

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Hasegawa, T., Okabe, A., Kato, Y., Ooshiro, A., Kawaide, H. & Natsume, M.
Bioassay-guided isolation of a novel chemoattractant for Ralstonia solanacearum in tomato root exudates. Journal of General Plant Pathology DOI: 10.1007/s10327-017-0752-5
論文リンク

Ye, Z., Yamazaki, K., Minoda, H., Miyamoto, K., Miyazaki, S., KAWAIDE, H., YAJIMA, A., NOJIRI, H., YAMANE, H. & OKADA, K.
In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2 Biosci. Biotechnol. Biochem.

Noguchi, C., Miyazaki, S. Kawaide, H., Gotoh, M., Yoshida, Y. and Aoyama, Y.
Characterization of moss ent-kaurene oxidase (CYP701B1) using a highly purified preparation. The Journal of Biochemistry (Tokyo)

Masahiro Natsume, Nobuya Tashiro, Akio Doi, Yatsuka Nishi & Hiroshi Kawaide
Effects of concanamycins produced by Streptomyces scabies on lesion type of common scab of potato.Journal of General Plant Pathology Volume 83, Issue 2, pp 78-82, 2017
論文リンク

2016年

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YE, Zhongfeng, NAKAGAWA, Kazuya, NATSUME, Masahiro, NOJIRI,Hideaki, KAWAIDE, Hiroshi & OKADA, Kazunori.
Biochemical synthesis of uniformly 13C-labeled diterpene hydrocarbons and their bioconversion to diterpenoid phytoalexins in planta.>Biosci. Biotechnol. Biochem. Vol.81 2017 (accepted)

Motoyama, K., Unno, H., hattori, A., Takaoka, T., Ishikita, H., Kawaide, H., Yoshimura, T. & Hennmo, H.
A single amino acid mutation converts (R)-5-diphosphomevalonate decarboxylase into a kinase.>J. Biol. Chem. 2017 (Feb. Issue) doi/10.1074/jbc.M116.752535
論文リンク

Toyomasu, T., Miyamoto, K., Shenton, M.R, Sakai, A., Sugawara, C., Horie, K., Kawaide, H., Hasegawa M., Chuba, M., Mitsuhashi, W., Yamane, H., Kurata, N. & Okada, K.
Characterization and evolutionary analysis of ent-kaurene synthase like genes from the wild rice species Oryza rufipogon.>Biochem. Biophys. Res. Commun. (2016) accepted for publication dated on 19th Oct. (BBRC-7403267)
論文リンク

Okada, M., Matsuda, Y., Mitsuhashi, T., Hoshino, S., Mori, T., Nakagawa, K., Quan, Z., Qin, B., Zhang, H., Hayashi, F., Kawaide, H. & Abe, I.
Genome-based discovery of an unprecedented cyclization mode in fungal sesterterpenoids biosynthesis.>J. Am. Chem. Soc. (2016) in press (DOI: 10.1221./jacs.6b05799). 論文リンク

Miyamoto, K., Fujita, M., Shenton, M., Akashi, S., Sugawara, C., Sakai, A., Horie, K., Hasegawa, M., Kawaide, H., Mitsuhashi, W., Nojiri, H., Yamane, H., Kurata, N., *Okada, K. & *Toyomasu, T.
Evolutionary trajectory of phytoalexin biosynthetic gene clusters in rice. >Plant J. (2016) in press DOI: 10.1111/tpj.13200. 論文リンク

Kazunori Okada, Hiroshi Kawaide, Koji Miyamoto, Sho Miyazaki, Ryosuke Kainuma, Honoka Kimura, Kaoru Fujiwara, Masahiro Natsume, Hideaki Nojiri, Masatoshi Nakajima, Hisakazu Yamane, Yuki Hatano, Hiroshi Nozaki & Ken-ichiro Hayashi HpDTC1, a Stress-Inducible Bifunctional Diterpene Cyclase Involved in Momilactone Biosynthesis, Functions in Chemical Defence in the Moss Hypnum plumaeforme. Scientific Reports (2016) 6:25316 | DOI: 10.1038/srep25316

2015年

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Sugawara, S., Mashiguchi,K., Tanaka, K., Hishiyama, S., Sakai, T., Hanada, K., Kinoshita-Tsujimura, K., Yu, H., Dai, X., Takebayashi, Y., Takeda-Kamiya, N., Kakimoto, T., Kawaide, H., Natsume, M., Estelle, M., Zhao, Y.,Hayashi, K., Kamiya, Y. and Kasahara, H.
Distinct characteristics of indole-3-acetic acid and phenyl acetic acid, two common auxins in plants. Plant & Cell Physiology (2015) 10.1093/pcp/pcv088
理研プレスリリース

川出 洋
菌類およびコケ・シダ類における生理活性ジテルペノイドの生合成研究
植物の生長調節50巻,12−20頁,2015

Miyazaki S., Kimura H., Natsume M., Asami T., Hayashi K., Kawaide H. and Nakajima M.
Analysis ot ent-kaurenoic acid by ultra performance liquid chromatography-tandem mass spectrometry. Biochemistry and Biophysics Reports (2015) 2, 103-107
論文リンク

Ogata, T., Okada, H., Kawaide, H., Takahashi, H., Seo, S., Matsuhara, I. and Matsushita, Y.
Involvement of NtERF3 in the cell death signaling pathway mediated by SIPK/WIPK and WRKY1 in tobacco plants. Plant Biology (Stuttgart Germany) (2015) 17 (in press)
論文リンク

Miyazaki, S., Nakajima, M. and Kawaide, H.
Hormonal diterpenoids derived from ent-kaurenoic acid are involved in the blue-light avoidance response of Physcomitrella patens. Plant Signal. Behav. (2015) 10(2), e989046_1-e989046_4
論文リンク

Shimada, S., Komatsu, T., Yamagami, A., Nakazawa, M., Matsui, M., Kawaide, H., Natsume,M., Osada, H., Asami, T. and Nakano, T.
Formation and dissociation of BSS1 protein complex regulates plant development via brassinosteroid signaling. Plant Cell (2015) 27, 375-390
理研プレスリリース

2014年

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Shimane, M., Ueno, Y., Morisaki, K., Oogami, S., Natsume, M., Hayashi, K., Nozaki, H. and Kawaide, H.
Molecular evolution of the substrate specificity of ent-kaurene synthases to adapt to gibberellin biosynthesis in land plants. Biochem. J. (2014) 462, 539-546
論文リンク

Tanaka, K., Hayashi, K., Natsume, M., Kamiya, Y., Sakakibara, H., Kawaide H. and Kasahara H.
UGT74D1 catalyzes the glucosylation of 2-oxindole-3-acetic acid inthe auxin metabolic pathway in Arabidopsis. Plant Cell Physiol. (2014) 55, 218-228
論文リンク

Kitaoka, N., Kawaide, H., Amano, N., Matsubara, T., Nabeta, K., Takahashi, K. and Matsuura, H.
CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation of 12-O-β-glucopyranosyl-jasmoyl-L-isoleucine as an additional metabolite. Phytochemistry (2014) 99, 6-13
論文リンク

Miyazaki, S., Toyoshima, H., Natsume, M., Nakajima, M. and Kawaide, H.
Blue-light irradiation up-regulates the ent-kaurene synthase gene and affects the avoidance response of protonemal growth in Physcomitrella patens. Planta (2014) 240, 117-124
論文リンク

Azami, Y., Hattori, A., Nishimura, H., Kawaide, H., Yoshimura, T., and Hemmi, H.
(R)-mevalonate 3-phosphate is an intermediate of the mevalonate pathway in Thermoplasma acidophilum. J. Biol. Chem. (2014) 289, 15957-15967

2013年

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Magome, H., Nomura, T., Hanada, A., Takeda-Kamiya, N., Ohnishi, T., Shinma, Y., Katsumata, T., Kawaide, H, Kamiya, Y. and Yamaguchi, S.
CYP714B1 and CYP714B2 encode gibberellin 13-oxidases that reduce gibberellin activity in rice. Proc. Natl. Acad. Sci. USA (2013) 110, 1947-1952
理研プレスリリース(PDF) 論文リンク

2012年

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Shimane, M., Sugai, Y., Kainuma, R., Natsume, M. and Kawaide, H.
Mevalonate-dependent enzymatic synthesis of amorphadiene driven by an ATP-regeneration system using polyphosphate kinase. Biosci. Biotechnol. Biochem. (2012) 76, 1558-1560
論文リンク

Toyama, T., Tahara, M., Nagamune, K., Arimitsu, K., Hamashima, Y., Palacpac, N.M., Kawaide, H., Horii, T., and Tanabe, K.
Gibberellin biosynthetic inhibitors make human malaria parasite Plasmodium falciparum cells swell and rupture to death. PLoS ONE (2012) 7, e32246
論文リンク

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菅井 佳宣、川出 洋
酵素合成法による完全13C標識化が可能にした多次元NMRによるmiltiradieneの構造解析 ―複雑精緻なテルペノイドの構造を明瞭に決定― 化学と生物 2012年 50巻 704-705

2011年

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Aoki, Y., Matsumoto, D., Kawaide, H. and Natsume, M.
Physiological role of germicidins in spore germination and hyphal elongation in Streptomyces coelicolor A3(2). J. Antibiot. (2011) 64, 607-611
論文リンク

Hashimoto, M., Katsura, H., Kato, R., Kawaide, H. and Natsume, M.
Effect of pamamycin-607 on secondary metabolite production by Streptomyces spp. Biosci. Biotechnol. Biochem. (2011) 75, 1722-1726
論文リンク

Ooshiro, A., Kaji, M., Katoh, Y., Kawaide, H. and Natsume, M.
Antibacterial activity of alkyl gallates and related compounds against Ralstonia solanacearum. J. Pestic. Sci. (2011) 36, 240-242
論文リンク

Kawaide, H., Hayashi, K., Kawanabe, R., Sakigi, Y., Natsume, M. and Nozaki, H.
Identification of the single amino acid involved in quenching the ent-kauranyl cation by a water molecule in ent-kaurene synthase of Physcomitrella patens. FEBS J. (2011) 278, 123-133
論文リンク

Sugai, Y. Miyazaki, S., Mukai, S., Yumoto, I., Natsume, M. and Kawaide, H.
Enzymatic total synthesis of gibberellin A4 from acetate. Biosci. Biotechnol. Biochem. (2011) 75, 128-135
(2011年BBB論文賞)
論文リンク

Katsumata, T., Fukazawa, J., Magome, H., Jikumaru, Y., Kamiya, Y., Natsume, M., Kawaide, H. and Yamaguchi, S.
Involvement of the CYP78A subfamily of cytochrome P450 monooxygenases in protonema growth and gametophore formation in the moss Physcomitrella patens. Biosci. Biotechnol. Biochem. (2011) 75, 331-336
論文リンク

Miyazaki, S., Katsumata, T., Natsume, M. and Kawaide, H.
The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. FEBS lett. (2011) 75, 331-336
論文リンク

Kitaoka, N., Matsubara, T., Sato, M., Takahashi, K., Wakuta, S., Kawaide, H., Matsui, H., Nabeta, K., and Matsuura, H.
Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the ocxidative catabolism of jasmonate. Plant Cell Physiol. (2011) 52, 1757-1765
論文リンク

Mashiguchi, K., Tanaka, K., Sakai, T., Sugawara, S., Kawaide, H., Natsume, M., Hanada, A., Yaeno, T., Shirasu, K., Yao, H., McSteen, P., Zhao, Y., Hayashi, K., Kamiya, Y. and Kasahara, H.
The main auxin biosynthesis pathway in Arabidopsis. Proc. Natl. Acad. Sci. USA (2011) 108, 18512-18517
論文リンク

Sugai, Y., Ueno, Y., Hayashi, K., Oogami, S., Toyomasu, T., Matsumoto, S., Natsume, M., Nozaki, H., and Kawaide, H.
Enzymatic 13C labeling and multidimensional NMR analysis of miltiradiene synthesized by bifunctional diterpene cyclase in Selaginella moellendorffii. J. Biol. Chem. (2011) 286, 42840-42847
論文リンク

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菅井 佳宣、川出 洋
生体反応を模倣した天然生物活性物質の酵素的全合成 BIOINDUSTRY 2011年 28巻 49-55

2010年

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Komatsu, T., Kawaide, H., Saito, C., Yamagami, A., Shimada, S., Nakazawa, M., Nakano, A., Tsujimoto, M., Natsume, M., Abe, H., Asami, T. and Nakano, T.
The Chloroplast protein BPG2 functions in brassinosteroid-mediated posttranscriptional accumulation of chloroplst rRNA. Plant J. (2010) 61, 409-422
論文リンク

Hayashi, K., Horie, K., Hiwatashi, Y., Kawaide, H., Yamaguchi, S., Hanada, A., Nakashima, T., Nakajima, M., Mander, L.N., Yamane, H., Hasebe, M., and Nozaki, H.
Endogenous diterpenes derived from ent-kaurene, a common gibberellin precursor, regulate protonema differentiation of the moss Physcomitrella patens. Plant Phyisiol. (2010) 153, 1085-1097
論文リンク

2009年

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Ooshiro, A., Hiradate, S., Kawaino, S., Takushi, T., Fujii, Y., Natsume, M. and Abe, H.
Identification and activity of ethyl gallate as an antimicrobial compound produced by Geranium carolinianum L. Weed Biol. Manag. (2009) 9, 169-172
論文リンク

2008年

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Kozone, I., Hashimoto, M., Graefe, U., Kawaide, H., Abe, H. and Natsume, M.
Structure-activity relationship of pamamycins: Effect of side chain length on aerial mycelium-inducing activity. J. Antibiot. (2008) 61, 98-102
論文リンク

Katsumata, T., Hasegawa, A., Fujiwara, T., Komatsu, T., Notomi, M., Abe, H., Natsume, M. and Kawaide, H.
Arabidopsis CYP85A2 catalyzes lactonization reactions in the biosynthesis of 2-deoxy-7-oxalactone brassinosteroids. Biosci. Biotechnol. Biochem. (2008) 49, 604-616
論文リンク

Okada, K., Kasahara, H., Yamaguchi, S., Kawaide, H., Kamiya, Y., Nojiri, H. and Yamane, H.
Genetic evidence for the role of isopentenyl diphosphate isomerases in the mevalonate pathway and plant development in Arabidopsis. Plant Cell Physiol. (2008) 49, 604-616
論文リンク

Sawada, Y., Katsumata, T., Kitamura, J., Kawaide, H., Nakajima, M., Asami, T., Nakaminami, K., Kurahashi, T., Mitsuhashi, W., Inoue, Y. and Toyomasu, T.
Germination of photoblastic lettuce seeds is regulated via the control of endogenous physiologically active gibberellin content, rather than of gibberellin responsiveness. J. Exp. Bot. (2008) 59, 3383-3393
論文リンク

Ko, KW., Lin, F., Katsumata, T., Sugai, Y., Miyazaki, S., Kawaide, H., Okada, K., Nojiri, H. and Yamane, H.
Functional identification of a rice ent-kaurene oxidase, OsKO2, using the Pichia pastoris expression system. Biosci. Biotechnol. Biochem. (2008) 72, 3285-3288
論文リンク

2007年

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Aoki, Y., Yoshida, M., Kawaide, H., Abe, H., and Natsume, M.
Structural determination of hypnosin, a spore germination inhibitor of phytopathogenic Streptomyces sp. causing root tumor in melon (Cucumis sp.). J. Agric. Food Chem. (2007) 55, 10622-10627
論文リンク

Aoki, Y., Yoshida, M., Kawaide, H., Abe, H., and Natsume, M.
Isolation and characterization of a spore germination inhibitor from Streptomyces sp. CB-1-1, a phytopathogen causing root tumor of melon. Biosci. Biotechnol. Biochem. (2007) 71, 986-992
論文リンク

Ooshiro, A. and Natsume, M.
Control of potato scab by Geranium carolinianum L. Weed Biol. Manag. (2007) 7, 124-127
論文リンク

Nozaki, H., Hayashi, K., Nishimura, N., Kawaide, H., Matsuo, A. and Takaoka, D.
Momilactone A and B as allelochemicals from moss Hypnum plumaeforme: First occurrence in bryophytes. Biosci. Biotechnol. Biochem. (2007) 71, 3127-3130
論文リンク

icon 総説

大城 篤、田場 聡、夏目 雅裕
Penicillium geastrivorusを利用した3種のサツマイモ病害の生物防除 BIOINDUSTRY 2011年 28巻 109-115

2006年

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Aoki, Y., Yoshida, Y., Yoshida, M., Kawaide, H., Abe, H. and Natsume, M.
Anthranilic acid, a spore germination inhibitor of phytopathogenic Streptomyces sp. B-9-1 causing root tumor of melon. Actinomycetologica (2006) 19, 48-54
論文リンク

Hayashi, K., Kawaide, H., Notomi, M., Sakigi, Y., Matsuo, A. and Nozaki, H.
Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens. FEBS Lett. (2006) 580, 6175-6181
論文リンク

Kawaide, H.
Biochemical and molecular analyses of gibberellin biosynthesis in fungi. Biosci. Biotechnol. Biochem. (2006) 70, 583-590
論文リンク

受賞関連

2014 Valent Bioscience Cooperation Best Poster Presentation Award (PGRSA/JSCRP 7th Joint Meeting)(2014年7月, サンフランシスコ)
受賞題目:Substrate Recognition-Product Diversity of ent-kaurene synthases in land plants

植物化学調節学会第47回大会ポスタ−賞(2012年10月,山形)
受賞題目:黄麹菌Aspergillus oryzae由来キメラ型新規ジテルペン合成酵素の反応解析

日本放線菌学会第27回大会ポスタ−賞(2012年9月,東京)
受賞題目:Streptomyces turegidiscabiesの植物毒素生産性と分類

社団法人 日本農芸化学会 欧文誌(Bioscience, Biotechnology and Biochemistry) 2011年BBB論文賞
受賞題目:Enzymatic total synthesis of gibberellin A4 from acetate

植物化学調節学会第46回大会ポスタ−賞(2011年11月,宇都宮)
受賞題目:酵素合成法による完全C-13標識化と多次元NMRを組合わせたmiltiradiene合成酵素の機能解析

社団法人 日本農芸化学会 日本農芸化学会2010年度大会トピックス賞(2010年3月,東京)
受賞題目:酢酸を原料とした活性型ジベレリンの酵素的全合成

TERPNET 2009 Good Presentation Award (9th International meeting: Biosynthesis and function of isoprenoids in plants, microorganisms and parasites)
受賞題目:Biosynthetic enzyme-dependent synthesis and labeling of natural terpenoids.

植物化学調節学会第42回大会ポスタ−賞(2007年10月,静岡)
受賞題目:酢酸を出発原料としたゲラニルゲラニル二リン酸の酵素的合成