東京農工大学大学院工学研究院生命機能科学部門 小関・山田・辻本研究室

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[2019 年]
[原著論文]
Sakaguchi, K., Isobe, C., Fujita, K., Ozeki, Y. and Miyahara, T. Production of novel red-purple delphinium flowers containing cyanidin-based anthocyanin using hybridization breeding. Horitic. J. 88(4): 514-520 (2019) (doi: 10.2503/hortj.UTD-100)
Tsujimoto, T., Nishihara, M., Osumi, Y., Hakamatsuka, T., Goda, Y., Uchiyama, N. and Ozeki, Y. Structural analysis of polygalaxanthones, C-glucosyl xanthones of Polygala tenuifolia roots. Chem. Pharm. Bull. 67(11), 1242–1247 (2019) (doi: 10.1248/cpb.c19-00608)
Tran, N.-H., Oguchi, T., Akatsuka, N., Matsunaga, E., Kawaoka, A., Yamada, A., Ozeki, Y., Watanabe, K. and Kikuchi, A. Development and evaluation of novel salt-tolerant eucalyptus trees by molecular breeding using an RNA-binding-protein gene derived from common ice plant (Mesembryanthemum crystallinum L.). Plant Biotechnol. J. 17(4): 801-811 (2019) (doi:10.1111/pbi.13016)

[2018 年]
[原著論文]
Miyahara, T., Sugishita, N., Ishida-Dei, M., Okamoto, E., Kouno, T., Cano, E.A., Sasaki, N., Watanabe, A., Tasaki, K., Nishihara, M. and Ozeki, Y. Carnation I locus has contains two chalcone isomerase genes involved in orange flower coloration. Breed. Sci. 68(4): 481-487 (2018) (doi:10.1270/jsbbs.18029)
Komatsu, R., Yamaguchi, T., Kobayashi, N., Ozeki, Y. and Sakurai, K. Synthesis of alkyne-tagged and biotin-tagged Sortin1 as novel photoaffinity probes. Bioorg. Med. Chem. Lett. 28(4): 1562-1565 (2018) (doi:10.1016/j.bmcl.2018.03.060)
Tokumoto, H., Shimomura, H., Hakamatsuka, T., Ozeki, Y. and Goda, Y. Fluorescence coupled with macro and microscopic examinations of morphological phenotype give key characteristics for identification of crude drugs derived from scorpion. Biol. Pharm. Bull. 41(4): 510-523 (2018) (doi:10.1248/bpb.b17-00801).
Totsuka, A., Okamoto, E., Miyahara, T., Kouno, T., Cano, E.A., Sasaki, N., Watanabe, A., Tasaki, K., Nishihara, M. and Ozeki, Y. Repressed expression of a gene for a basic helix-loop-helix protein causes a white flower phenotype in carnation. Breed. Sci. 68(1): 139-143 (2018) (doi:10.1270/jsbbs.17072).

[2017 年]
[原著論文]
Ishii, I., Sakaguchi, K., Fujita, K., Ozeki, Y. and Miyahara, T. A double knockout mutant of acyl-glucose-dependent anthocyanin glucosyltransferase genes in Delphinium grandiflorum. J. Plant Physiol. 216(1): 74-78 (2017) (DOI: 10.1016/j.jplph.2017.05.009).

[2016 年]
[原著論文]
Miyahara, T., Miyake, N., Sawahuji, K., Kitta, K., Nakamura, K., Kondo, K. and Ozeki, Y. Wheat DNA fragmentation of commercial processed foods. Jpn. J. Food Chem. Safety23(3): 141-148 (2016).
Miyahara, T., Hamada, A., Okamoto, M., Hirose, Y., Sakaguchi, K., Hatano, S. and Ozeki, Y. Identification of flavonoid 3'-hydroxylase in the yellow flower of Delphinium zalil. J. Plant Physiol. 202(3): 92-96 (2016) (DOI: 10.1016/j.jplph.2016.07.013).
Tokumoto, H., Shimomura, H., Hakamatsuka, T., Ozeki, Y. and Goda, Y. Detection of Nicotiana tabacum leaf contamination in pharmaceutical products. Biol. Pharm. Bull. 39(8): 1263-1272 (2016) (doi.org/10.1248/bpb.b15-01004).

[2015 年]
[原著論文]
Miyagawa, N., Miyahara, T., Okamoto, M., Hirose, Y., Sakaguchi, K., Hantano, S. and Ozeki, Y. Dihydroflavonol 4-reductase activity is associated with the intensity of flower colors in delphinium. Plant Biotechnol. 32(3): 249-255 (2015) (DOI:10.5511/plantbiotechnology.15.0702b).
Ito, A., Taguchi, T., Mogi, T., Mitsumori, Y., Tadenuma, T., Wake, H., Tanaami, T., Akiyama, H., Teshima, R., Sasaki, N., Yamada, A. and Ozeki, Y. Identification of genetically modified organisms by detection of target gene pattern using DNA microarrays. Jpn. J. Food Chem. Safety 22(2): 133-138 (2015).

[2014 年]
[原著論文]
Miyahara, T., Tani, T., Takahashi, M., Nishizaki, Y., Ozeki, Y. and Sasaki, N. Isolation of anthocyanin 7-O-glucosyltransferase from Canterbury bells (Campanula medium). Plant Biotechnol. 31(5):555-559 (DOI:10.5511/plantbiotechnology.14.0908a) (2014).
Sasaki, N., Nishizaki, Y., Ozeki, Y. and Miyahara, T. The role of acyl-glucose in anthocyanin modifications. Molecules 19: 18747-18766 (DOI:10.3390/molecules191118747) (2014).
Suzuki, M., Miyahara, T., Tokumoto, H., Hakamatsuka, T., Goda, Y., Ozeki, Y. and Sasaki, N. Transposon-mediated mutation of CYP76AD3 affects betalain synthesis and produces variegated flowers in four o’clock (Mirabilis jalapa). J. Plant Physiol. 171(8):1586-1590 (DOI:10.1016/j.jplph.2014.07.010) (2014).
Nishizaki, Y., Sasaki, N., Yasunaga, M., Miyahara, T., Okamoto, E., Okamoto, M., Hirose, Y. and Ozeki, Y. Identification of the glucosyltransferase gene that supplies the p-hydroxybenzoyl-glucose for 7-polyacylation of anthocyanin in delphinium. J. Exp. Bot. 65(9): 2495-2506 (doi:10.1093/jxb/eru134) (2014).
Yagi, M., Kosugi, S., Hirakawa, H., Ohmiya, A., Tanase, K., Harada, T., Kishimoto, K., Nakayama, M., Ichimura, K., Onozaki, T., Yamaguchi, H., Sasaki, N., Miyahara, T., Nishizaki, Y., Ozeki, Y., Nakamura, N., Suzuki, T., Tanaka, Y., Sato, S., Shirasawa, K., Isobe, S., Miyamura, Y., Watanabe, A., Nakayama, S., Kishida, Y., Kohara, M., and Tabata, S. Sequence analysis of the genome of carnation (Dianthus caryophyllus L.). DNA Res. 21(2): 231-241 (DOI:10.1093/dnares/dst053) (2014).
Wakana, D , Kato, H., Momose, T., Sasaki, N., Ozeki, Y. and Goda, Y. NMR-based characterization of a novel yellow chlorophyll catabolite, Ed-YCC, isolated from Egeria densa. Tetrahedron Lett. 55(18): 2982-2985 (2014) (DOI: 10.1016/j.tetlet.2014.03.114).
Miyagawa, N., Nishizaki, Y., Miyahara, T., Okamoto, M., Hirose, Y., Ozeki, Y. and Sasaki, N. Sequence variations in the flavonoid 3′,5′-hydroxylase gene associated with reddish flower phenotypes in three delphinium varieties. Plant Biotechnol., 31(1): 83-87 (2014) (DOI: 10.5511/plantbiotechnology.13.1203c).
Nakamura, R., Nakamura, R., Adachi, R., Hachisuka, A., Yamada, A., Ozeki, Y. and Teshima, R. Differential analysis of protein expression in RNA-binding-protein transgenic and parental rice seeds cultivated under salt stress. J. Proteome Res., 13(2): 489-495 (2014). (DOI:10.1021/pr4006487).

[2013 年]
[原著論文]
Momose, M., Itoh, Y., Umemoto, N., Nakayama, M. and Ozeki, Y. Molecular characterization of carnation (Dianthus caryophyllus) S locus alleles for flower color intensity. Breeding Sci., 63(4): 435-440 (2013) (DOI:10.1270/jsbbs.63.435).
Nishizaki, Y., Yasunaga, M., Okamoto, E., Okamoto, M., Hirose, Y., Yamaguchi, M., Ozeki, Y. and Sasaski, N. p-Hydroxybenzoyl-glucose is a “Zwitter donor” for the biosynthesis of polyacyl-anthocyanin in delphinium. Plant Cell, 25(10): 4150-4165 (2013) (DOI: 10.1105/tpc.113.113167).
Sasaki, N., Matsuba, Y., Abe, Y., Okamura, M., Momose, M., Umemoto, N., Nakayama, M., Itoh, Y. and Ozeki, Y. Recent advances in understanding the anthocyanin modification steps in carnation flowers. Sci. Hortic., 163(1): 37-45 (DOI: 10.1016/j.scienta.2013.07.029) (2013).
Momose, T. and Ozeki, Y. Regulatory effect of stems on sucrose-induced chlorophyll degradation and anthocyanin synthesis in Egeria densa leaves. J. Plant Res., 126(5): 859-867 (2013) (DOI:10.1007/s10265-013-0581-3).
Nakatsuka, T., Yamada, E., Takahashi, H., Imamura, T., Suzuki, M., Ozeki, Y., Tsujimura, I., Saito, M., Sakamoto, Y., Sasaki, N. and Nishihara, M. Genetic engineering of yellow betalain pigments beyond the species barrier. Scientific Reports, 3: 1970 (DOI: 10.1038/srep01970) (2013).
Luang, S., Cho, J., Mahong, B., Opassiri, R., Akiyama, T., Phasai, K., Komvongsa, J., Sasaki, N., Hua, Y., Matsuba, Y., Ozeki, Y., Jeon, J.-S., Ketudat Cairns, J.R. Rice Os9BGlu31 is a transglucosidase with the capacity to equilibrate phenylpropanoid, flavonoid, and phytohormone glycoconjugates. J. Biol. Chem., 288(14): 10111-10123 (2013) (DOI: 10.1074/jbc.M112.423533).
Momose, M., Nakayama, M., Itoh, Y., Umemoto, N., Toguri, T. and Ozeki, Y. An active hAT transposable element causing bud mutation of carnation by insertion into flavonoid 3'-hydoroxylase gene. Mol. Gen. Genomics, 288(3): 175-184 (2013) (DOI: 10.1007/s00438-013-0742-z).
Okamura, M., Nakayama, M., Umemoto, N., Cano, E. A., Hase, Y., Nishizaki, Y., Sasaki, N. and Ozeki, Y. Crossbreeding of a metallic color carnation and diversification of the peculiar coloration by ion-beam irradiation. Euphytica, 191: 45-56 (2013) (DOI: 10.1007/s10681-012-0859-x).
Miyahara, T., Sakiyama, R., Ozeki, Y. and Sasaki, N. Acyl-glucose-dependent glucosyltransferase catalyzes the final step of anthocyanin formation in Arabidopsis. J. Plant Phyisiol., 170(6): 619-624 (2013) (DOI: 10.1016/j.jplph.2012.12.001).
Yu, X., Kikuchi, A., Shimazaki, T., Yamada, A., Ozeki, Y., Matsunaga, E., Ebinuma, H. and Watanabe, K. N. Assessment of the salt tolerance and environmental biosafety of Eucalyptus camaldulensis harboring a mangrin transgene. J. Plant Res., 126(1): 141-150 (DOI 10.1007/s10265-012-0503-9) (2013).
[総説他]
小関良宏、松葉由紀、佐々木伸大.フラボノイドの生合成・アントシアニンの生合成 — 植物色素フラボノイド.(武田幸作、齋藤規夫、岩科 司編)文一書店,331-343 (2013)

[2012 年]
[原著論文]
Mikawa, T., Kubota, H., Ozeki, Y., Yoshida, M., Nakanishi, T., Sato, K. and Akiyama, H. Determination of sodium stearoyl lactylates in foods using HPLC after derivatization with 2-nitrophenyl hydrazine. Jpn. J. Food Chem. Safety, 19(3): 178-184 (2012).
Ito, A., Taguchi, T., Mogi, T., Wake, H., Tanaami, T., Akiyama, H., Teshima, R., Sasaki, N., Yamada, A. and Ozeki, Y. Comparison of signal enhancement techniques using DNA microarrays for screening GM crops. Jpn. J. Food Chem. Safety, 19(2): 141-148 (2012).
Kubota, H., Sato, K., Sasaki, N., Kawamura, Y., Ozeki, Y. and Akiyama H. Formation of volatile halogenated compounds in fresh-cut cabbage treated with sodium hypochlorite. Jpn. J. Food Chem. Safety, 19(2): 94-103 (2012).
Ochi, K., Nishizawa, T., Inaoka, T., Yamada, A., Hashimoto, K., Okamoto, S. and Ozeki, Y. Heterologous expression of plant RelA/SpoT homolog results in increased stress tolerance in Saccharomyces cerevisiae by accumulation of the bacterial alarmone ppGpp. Microbiol., 158 (8): 2213-2224 (2012)(DOI 10.1099/mic.0.057638-0).
Miyahara, T., Takahashi, M., Ozeki, Y. and Sasaki, N. Isolation of an acyl-glucose-dependent anthocyanin 7-O-glucosyltransferase from the monocot Agapanthus africanus. J. Plant Phyisiol., 169(8): 1321-1326 (2012) (DOI: 10.1016/j.jplph.2012.05.004).
Sasaki,N., Nishizaki, Y., Uchida, Y., Wakamatsu, E., Umemoto, N., Momose, M., Okamura, M., Yoshida, H., Yamaguchi, M., Nakayama, M., Ozeki, Y. and Itoh, Y. Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations. Plant Biotechnol., 29(3): 223-227 (2012) (DOI: 10.5511/plantbiotechnology.12.0120a).
Morita,Y., Saito, R., Ban, Y., Tanikawa, N., Kuchitsu, K., Ando, T., Yoshikawa, M., Habu, Y., Ozeki, Y. and Nakayama, M. Tandemly arranged chalcone synthase A genes contribute to the spatially regulated expression of siRNA and the natural bicolor floral phenotype in Petunia hybrida. Plant J., 70: 739-749 (2012) (DOI: 10.1111/j.1365-313X.2012.04908.x).

[2011 年]
[原著論文]
Nishizaki, Y., Matsuba, Y., Okamoto, E., Okamura, M., Ozeki, Y. and Sasaki, N. Structure of the acyl-glucose-dependent anthocyanin 5-O-glucosyltransferase gene in carnations and its disruption by transposable elements in some varieties. Mol. Gen. Genomics, 286(3): 383-394 (2011) (DOI: 10.1007/s00438-011-0655-7).
Terato, M., Ishikawa, A., Yamada, K., Ozeki, Y. and Kitamura, Y. Increased furanocoumarin production by Glehnia littoralis roots induced via Agrobacterium rhizogenes infection. Plant Biotechonol., 28(3): 317-321 ((2011)DOI: 10.5511/plantbiotechnoloby.11.0330a).
Miyahara, T., Matsuba, Y., Ozeki, Y. and Sasaki, N. Identification of genes in Arabidopsis thaliana with homology to a novel acyl-glucose dependent glucosyltransferase of carnations. Plant Biotechonol., 28(3): 311-315 (DOI: 10.5511/plantbiotechnoloby.11.0111b) (2011).
浅子洋一, 大脇裕介, 小関良宏, 佐々木伸大, 阿部裕, 百瀬忠征, 下村講一郎. 地下部内部全体にペラルゴニジンを主たるアントシアニジンとして蓄積するダイコン(Raphanus sativus L.)の中間母本「乾谷」とその遺伝様式. 育種学研究,13 : 65-73 (2011)
[総説]
Ozeki, Y., Matsuba, Y., Abe, Y., Umemoto, N. and Sasaki, N. Pigment Biosynthesis I. Anthocyanins In: Ashihara, H., Crozier, A. and Komamine, A. (Eds), Plant Metabolism and Biotechnology, John Wiley & Sons, Ltd, pp. 313-333 (2011).
松葉由紀、佐々木伸大、小関良宏. 花の色とアントシアニン分子の修飾-新たな糖修飾酵素-.バイオサイエンスとインダストリー,69 (3): 190 - 191 (2011).
佐々木伸大、松葉由紀、小関良宏. 植物二次代謝に関わる新規糖転移酵素の発見.バイオサイエンスとインダストリー,69 (3): 200 - 203 (2011).

[2010 年]
[原著論文]
Sekiguchi, H., Ozeki, Y. and Sasaki, N. In vitro synthesis of betaxanthins using recombinant DOPA 4,5-dioxygenase and evaluation of their radical-scavenging activities. J. Agri. Food Chem., 58(23): 12504-12509 (2010).
Akiyama, H., Makiyama, D., Nakamura, K., Sasaki, N., Minegishi, Y., Mano, J., Kitta, K., Ozeki, Y. and Teshima, R. A novel detection system for the genetically modified canola (Brassica rapa) line RT73. Anal. Chem., 82(23): 9909-9916 (2010).
Matsuba, Y., Sasaki, N., Tera, M., Okamura, M., Abe, Y., Okamoto, E., Nakamura, H., Funabashi, H., Saito, M., Matsuoka, H., Nagasawa, K and Ozeki, Y. A novel glucosylation reaction on anthocyanins catalyzed by acyl-glucose dependent glucosyltransferase in the petals of carnation and delphinium. Plant Cell, 22(10): 3374-3389 (2010) (DOI: 10.1105/tpc.110.077487).
Momose, M., Abe, Y. and Ozeki, Y. Miniature inverted-repeat transposable elements of Stowaway are active in potato. Genetics, 186(1): 59-66 (2010).
Wako, W., Kimura, S., Chikagawa,Y. and Ozeki, Y. Characterization of MYB proteins acting as transcriptional regulatory factors for carrot phenylalanine ammonia-lyase gene (DcPAL3). Plant Biotechonol., 27(2): 131-139 (2010).
Miyahara, T., Satoh, S., Maeda, K., Kimura, S., Sasaki, N. and Ozeki, Y. Isolation of Daucus carota ethylene insensitive3-like (DcEIL) involved in stress-inducible DcMYB1 expression in suspension-cultured carrot cells. Plant Biotechonol., 27(1): 91-97 (2010).
[総説]
小関良宏. 遺伝子組換え食品の安全性評価.日本農薬学会誌,35 (3): 383 - 388 (2010).

[2009 年]
[原著論文]
Sasaki, N., Abe, Y., Goda, Y., Adachi, T., Kasahara, K. and Ozeki, Y. Detection of DOPA 4,5-dioxygenase (DOD) activitiy using the recombinant protein prepared from Escherichia coli cells harboring cDNA encoding DOD from Mirabilis jalapa. Plant Cell Physiol., 50 (5): 1012-1016 (2009).
Ishikawa, A., Kuma, T., Sasaki, H., Sasaki, N., Ozeki, Y. Kobayashi, N. and Kitamura, Y. Constitutive expression of bergaptol O-methyltransferase in Glehnia littoralis to cell cultures. Plant Cell Rep., 28 (1): 257-265 (2009).
[総説他]
小関良宏. 動けない植物が動物から食われないように — 防御物質としての植物毒.生物の科学「遺伝」,63 (2): 54-59 (2009).
小関良宏 (分担執筆).遺伝子組換え食品 — 食品安全の事典.(日本食品衛生学会編)朝倉書店,531-536 (2009).

[2008 年]
[原著論文]
Abe, Y., Sawada, A., Momose, T., Sasaki, N., Kawahara, N., Kamakura, H., Goda, Y. and Ozeki, Y. Structure of an anthocyanin-anthocyanin dimer molecule in anthocyanin-producing cells of a carrot suspension culture. Tetrahedron Lett., 49(51): 7330-7333 (2008).
Abe, Y., Shoji, T., Kawahara, N., Kamakura, H., Kanda, T., Goda, Y., and Ozeki, Y. Structural characterization of a procyanidin tetramer and pentamer from the apple by low-temperature NMR analysis. Tetrahedron Lett., 49(45): 6413-6418 (2008).
Kimura, S., Oyanagi, M., Fukuda, T., Ohno, Y., Hongo, C., Itoh, Y., Koda, T. and Ozeki, Y. Role of miniature inverted repeat transposable elements inserted into the promoter region of a carrot phenylalanine ammonia-lyase gene and its gene expression. Plant Biotechonol., 25(5): 473-481 (2008).
Kimura, S., Chikagawa, Y., Kato, M., Maeda, K. and Ozeki, Y. Upregulation of the promoter activity of the carrot phenylalanine ammonia-lyase gene (DcPAL3) was caused by new members of the transcriptional regulatory proteins, DcERF1 and DcERF2, which bound to the GCC-box homolog and acted as an activator to the DcPAL3 promoter. J. Plant Res., 121(5): 499-508 (2008).
Abe, Y., Tera, M., Sasaki, N., Okamura, M., Umemoto, N., Momose, M., Kawahara, N., Kamakura, H., Goda, Y., Nagasawa, K., and Ozeki, Y. Detection of 1-O-malylglucose: pelargonidin 3-O-glucose-6¢¢-O-malyltransferase activity in carnation (Dianthus caryophyllus) . Biochem. Biophys. Res. Comm., 373(3): 473-477 (2008).
Matsuba, Y., Okuda Y., Abe, Y., Kitamura, Y., Terasaka, K., Mizukami, H., Kamakura, H., Kawahara, N., Goda, Y., Sasaki, N., and Ozeki, Y. Enzymatic preparation of 1-O-hydroxycinnamoyl-b-D-glucoses and their application to the study of 1-O-hydroxycinnamoyl-b-D-glucose-dependent acyltransferase in anthocyanin-producing cultured cells of Daucus carota and Glehnia littoralis. Plant Biothechnol., 25(4): 369-375(2008).

[2007 年]
[原著論文]
Saito, R., Kuchitsu, K., Ozeki, Y., and Nakayama, M. Spatiotemporal metabolic regulation of anthocyanin and related compounds during the development of marginal picotee petals in Petunia hybrida (Solanaceae). J. Plant Res., 120(4): 563-568 (2007).
Ishikawa, A., Kitamura, Y., Ozeki, Y. and Watanabe, M. Different responses of shoot and root cultures of Glehnia littoralis to yeast extract. J. Nat. Med., 61: 30-37 (2007).

[2006 年]
[原著論文]
Nagira, Y., Shimamura, K., Hirai, S., Shimanuki, M., Kodama, H. and Ozeki, Y. Identification and characterization of genes induced for anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots using suppression subtractive hybridization, cDNA microarrays, and RNAi techniques. J. Plant Res., 119: 217-230 (2006).
Maeda, K. and Ozeki, Y. Self-interaction of DcMYB1 involved in stress-inducible DcPAL1 expression. Plant Biothechnol., 23: 219-225 (2006).
Maeda, K., Kimura, S., Noma, W. and Ozeki, Y. A promoter analysis of the DcMYB1 gene that encodes the transcriptional activator of the stress-inducible DcPAL1 gene. Plant Biothechnol., 23: 203-209 (2006).
Nagira, Y., Ikegami, K., Koshiba, T. and Ozeki, Y. Effect of exogenous and endogenous ABA upon anthocyanin synthesis in regenerated torenia shoots.J. Plant Res., 119: 137-144 (2006).
Saito, R., Fukuta, N., Ohmiya, A., Itoh, Y., Ozeki, Y., Kuchitsu, K. and Nakayama, M. Regulation of anthocyanin biosynthesis involved in the formation of marginal picotee petals in Petunia. Plant Science, 170: 828-834 (2006).

[2005 年]
[原著論文]
Sasaki, K., Umetsu, H., Yamada, A., Kamada, H. and Ozeki, Y. Detection of NPTII in genetically modified foods with ELISA. Jpn. J. Food Chem., 12: 140-144 (2005).
Sasaki, N., Abe, Y., Wada, K., Koda, T., Goda, Y., Adachi, K. and Ozeki, Y. Amaranthin in feather cockscombs is synthesized via glucuronylation at the cyclo-DOPA glucoside step in the betacyanin biosynthetic pathway. J. Plant Res., 118: 439-442 (2005).
Maeda, K., Kimura, S., Demura, T., Takeda, J. and Ozeki, Y. DcMYB1 acts as a transcriptional activator of the carrot phenylalanine ammonia-lyase gene (DcPAL1) in response to elicitor treatment, UV-B irradiation and the dilution effect.Plant Mol. Biol., 59: 739-752(2005).
Ishikawa, A., Kitamura, Y., Ozeki, Y., Itoh, Y., Yamada, A. and Watanabe, M. Post-stress metabolism involves umbelliferone production in anthocyanin-producing and non-producing cells of Glehnia littoralis suspension cultures. J. Plant Physiol., 162: 703-710 (2005).
Sasaki, N., Wada, K., Koda, T., Kasahara, K., Adachi, K. and Ozeki, Y. Isolation and characterization of cDNAs encoding the enzyme having glycosylatransferase activity to cyclo-DOPA from four o’clocks and feather cockscomb. Plant Cell Physiol., 46: 671-675 (2005).
Ogasawara, T., Chikagawa, Y., Arakawa, F., Nozaki, A., Itoh, Y., Sasaki, K., Umetsu, H., Watanabe, T., Akiyama, H., Maitani, T., Toyada, M., Kamada, H., Goda, Y. and Ozeki, Y. Mutations of the transgene of Roundup Ready® soybeans, which could result in the loss of the glyphosate-tolerant phenotype, might be reduced using an artificial selection bias. J. Health Sci., 51: 197-201 (2005).
[総説]
小関良宏. 遺伝子組換え食品における安全性評価.FFI ジャーナル,210: 647-654 (2005).

[2004 年]
[原著論文]
Nozaki, A., Yamada, A., Mimura, T. and Ozeki, Y. Role of eEF1A in the mangrove plant Brugiera sexangula under salt and osmotic stress. Mar. Biotechnol., 6: S48-S51 (2004).
Yamada, A., Mimura, T. and Ozeki, Y. Novel salt-resistant protein, mangrin, isolated from a mangrove plant, Brugiera sexangula. Mar. Biotechnol., 6: S52-S55 (2004).
Ogata, J., Itoh, Y., Ishida, M., Yoshida, H. and Ozeki, Y. Cloning and heterologous expression of a cDNA encoding flavonoid glucosyltransferases from Dianthus caryophyllus. Plant Biothechnol., 21: 367-375 (2004).
Ogasawara, T., Arakawa, F., Watanabe, T., Akiyama, H., Hino, H., Maitani, T., Goda, Y. and Ozeki, Y. Genomic DNA fragmentation of genetically modified corn during food processing. Jpn. J. Food Chem., 11: 137-143 (2004).
Nagira, Y. and Ozeki, Y. A system in which anthocyanin synthesis is induced in regenerated torenia shoots. J. Plant Res., 117: 377-383 (2004).
Sasaki, N., Adachi, T., Koda, T. and Ozeki, Y. Detection of UDP–glucose:cyclo-DOPA 5-O-glucosyltransferase activity in four o'clocks (Mirabilis jalapa L.). FEBS Letters, 568: 159-162 (2004).
Yoshida, H., Itoh, Y., Iwashina, T., Yamaguchi,M. and Ozeki, Y. Differences in chalcononaringenin 2'-O-glucoside content in the petals of carnations (Dianthus caryophyllus) bearing yellow flowers. Scientia Horticulturae, 99: 175-186 (2004).
Yoshida, H., Akimoto, H., Yamaguchi, M., Shibata, M., Habu, Y., Iida, S. and Ozeki, Y. Alteration of methylation profiles in distinct cell lineages of the layers during vegetative propagation in carnation (Dianthus caryophyllus). Euphytica, 135: 247-253 (2004).
[総説他]
小関良宏. 遺伝子組換え種子植物とその掛け合わせ品種.食品衛生研究,54: 13-18 (2004).
伊藤佳央、小関良宏. 花の模様は遺伝子のしわざだった! 化学,59 (12) : 40-43 (2004).
小関良宏・伊藤佳央 (分担執筆). 色素生合成系の酵素遺伝子の解析法 — 植物色素研究法.(植物色素研究会編)大阪公立大学共同出版会,149-159 (2004)
小関良宏 (分担執筆). 食品衛生法と食品安全基本法 − 新しい遺伝子組換え体 (GMO) の安全性評価システムガイドブック.(田部井豊・日野明寛・矢木修身編)エヌ・ティー・エス社,171-184 (2004)
小関良宏 (分担執筆). 食品(種子植物)としての安全性評価 − 新しい遺伝子組換え体 (GMO) の安全性評価システムガイドブック.(田部井豊・日野明寛・矢木修身編)エヌ・ティー・エス社,232-273 (2004)

[2003 年]
[原著論文]
Ogasawara, T., Arakawa, F., Akiyama, H., Goda, Y. and Ozeki, Y. Fragmentation of DNAs of processed foods made from genetically modified soybeans. Jpn. J. Food Chem., 10: 155-160 (2003).
Itoh, Y., Hasebe, M., Davies, E., Takeda, J. and Ozeki, Y. Survival of Tdc transposable elements of the En/Spm superfamily in the carrot genome. Mol. Gen. Genomics, 269: 49-59 (2003).
Ozeki, Y., Chikagawa, Y., Kimura, S., Soh, H.-c., Maeda, K., Pornsiriwong, W., Kato, M., Akimoto, H., Oyanagi, M., Fukuda, F., Koda, T., Itoh, Y., Yamada, A., Davies, E., Ueno, H. and Takeda, J. Putative cis-elements in the promoter region of the carrot phenylalanine ammonia-lyase gene induced during anthocyanin synthesis. J. Plant Res., 116: 155-159 (2003).
Yamada, A., Nozaki, A., Sano, E, Mimura, T. and Ozeki, Y. Expression of mangrove eEF1A enhances tolerance to salt and osmotic stress in Escherichia coli. Plant Biotechnol., 20: 81-85 (2003).
Yamada, A., Tsutsumi, K., Tanimoto, S. and Ozeki, Y. Plant RelA/SpoT homolog confers salt tolerance in Eschericia coli and Saccharomyces cerevisiae. Plant Cell Physiol., 44: 3-9 (2003).

[2002 年]
[原著論文]
Yamada, A., Sekiguchi, M., Mimura, T. and Ozeki, Y. The role of plant CCTα genes in salt- and osmotic-stress tolerance. Plant Cell Physiol., 43: 1043-1048 (2002).
Yamada, A., Sekiguchi, M., Mimura, T. and Ozeki, Y. Expression of plant CCT genes enhances salt and osmotic tolerance in Escherichia coli. Plant Biotechnol., 19: 191-196 (2002).
Takeda, J., Itoh, Y., Maeda, K. and Ozeki, Y. Assignment of UVB-responsive cis-element and protoplastization- (dilution-) and elicitor-responsive ones in the promoter region of a carrot phenylalanine ammonia-lyase gene (gDcPAL1). Photochem. Photobiol., 76: 232-238 (2002).
Yamada, A., Saitoh, T., Mimura, T. and Ozeki, Y. Expression of mangrove allen oxide cyclase enhances salt tolerance in Escherichia coli, yeast and tobacco cells. Plant Cell Physiol., 43: 903-910 (2002).
Yamada, A., Saitoh, T., Mimura, T. and Ozeki, Y. Detection of differences in mRNA expression regulated by salt-stress in mangrove cultured cells. Plant Biotechnol. 19: 145-148 (2002).
Itoh, Y., Higeta, D., Suzuki, A., Yoshida, H. and Ozeki, Y. Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus). Plant Cell Physiol., 43: 578-585 (2002).
Itoh, Y. and Ozeki, Y. Identification of genetic alterations in carrot cell lines using transposable elements belonging to the Tdc1 family. Plant Biotechnol. 19: 37-44 (2002).

[2001 年]
[原著論文]
Mato, M., Onozaki, T., Ozeki, Y., Higeta, D., Itoh, Y., Hisamatsu, T., Yoshida, H. and Shibata, M. Flavonoid biosynthesis in pink-flowered cultivars derived from ‘William Sim’ carnation. (Dianthus caryophyllus). J. Japan Soc. Hort. Sci. , 70 : 315-319 (2001).
[総説他]
小関良宏. 植物における遺伝子組換え技術.『生物の科学 遺伝』,55 (6) : 29-34 (2001).
小関良宏. 遺伝子組換え食品の現状.臨床栄養,98: 266-272 (2001).
小関良宏. 遺伝子組換え食品の現状と安全性.月刊「厚生」,2001 年 6 月号 15-17

[2000 年]
[原著論文]
Yoshimoto, Y., Higeta, D., Itoh, Y., Yoshida, H., Hasebe, M. and Ozeki, Y. Isolation and characterization of a cDNA for phenylalanine ammonia-lyase (PAL) from Dianthus caryophyllus (carnation). Plant Biotechnol., 17 : 325-329 (2000).
Mato, M., Onozaki, T., Ozeki, Y., Higeta, D., Itoh, Y., Yoshimoto, Y., Ikeda, H., Yoshida, H. and Shibata, M. Flavonoid biosynthesis of white-flowered Sim carnations (Dianthus caryophyllus). Scientia Horticulturae, 84 : 333-347 (2000).

東京農工大学工学研究院生命機能科学部門
小関・山田・宮原研究室