Ohno & Nakamura Laboratory

藤田 恭子 Kyoko Fujita

E-mail: kyokof (at) cc.tuat.ac.jp
※スパム対策のため、「@」を「(at)」と表記しています。


学歴

1999年 3月 東京農工大学工学部生命工学科卒業
2001年 3月 東京農工大学大学院工学府生命工学専攻博士前期課程修了
2004年 3月 東京農工大学大学院工学府生命工学専攻博士後期課程修了

職歴

2001年 4月 - 2004年 3月 日本学術振興会特別研究員(DC1)
2002年 12月 - 2003年 5月 カリフォルニア工科大学(アメリカ合衆国) 訪問研究員
2004年 6月 - 2006年 2月 Monash大学(オーストラリア) 博士研究員
2006年 5月 - 2008年 3月 東京農工大学工学府 科研費等研究支援研究員
2008年 4月 - 2010年 1月 日本学術振興会 特別研究員(RPD)
2010年 2月 - 2011年 3月 東京農工大学工学府 産学官連携研究員
2011年 4月 - 2013年 9月 東京農工大学工学研究院 特任助教
2013年 10月 - 東京農工大学工学研究院 講師

研究分野

電気化学・生体高分子


キーワード

タンパク質・イオン液体・光導波路分光法・バイオ燃料電池


原著論文

32. Sugar chain-binding specificity and native folding state of lectin preserved in hydrated ionic liquids, K. Fujita, M. Sanada, and H. Ohno, Chem. Comm., 2015, 51, 10883-10886. Link

31. Is seven a minimum number of water molecules per ion pair for assured biological activity in ionic liquid/water mixtures?, H. Ohno, K. Fujita Y. Kohno, Physical Chemistry Chemical Physics, 2015, 17, 14454-14460.Link

30. A simple recovery process for biodegradable plastics accumulated in cyanobacteria treated with ionic liquids, D. Kobayashi, K. Fujita N. Nakamura and H. Ohno, Applied Microbiology and Biotechnology, 2015,99, 1647-1653

29. 2-(Trimethylazaniumyl)ethyl hydrogen phosphate (phosphocholine) monohydrate, Y. Nikawa, K. Fujita and H. Ohno, Acta Cryst., 2014, E70, o549, o549. Link

28. Cold crystallisation behaviour of water molecules in ionic liquids as a screening method to evaluate biocompatibility of the hydrated ionic liquids, K. Fujita, Y. Nikawa, and H. Ohno, Chem. Commun., 2013, 49, 3257-3259. Link

27. Direct dissolution of wet and saliferous marine microalgae by polar ionic liquids without heating, K. Fujita, D. Kobayashi, N. Nakamura, and H. Ohno, Enzyme and Microbial Tech., 2013, 52, 199-202. Link

26. Contracting cardiomyocytes in hydrophobic room-temperature ionic liquid, T. Hoshino, K. Fujita, A. Higashi, K. Sakiyama, H. Ohno, and K. Morishima, Biochem. Biophys. Res. Commun., 2012, 427, 379-384. Link

25. Stable G-quadruplex structure in a hydrated ion pair: cholinium cation and dihydrogen phosphate anion, K. Fujita and H. Ohno, Chem. Commun., 2012, 48, 5751-5753. Link

24. Electrochemical analysis of electrode-immobilized dehydrogenases in hydrated choline dihydrogen phosphate type ionic liquid, K. Fujita, N. Nakamura, K. Murata, K. Igarashi, M. Samejima, and H. Ohno, Electrochim. Acta, 2011, 56, 7224-7227. Link

23. Enzymatic activity and thermal stability of metallo proteins in hydrated ionic liquids, K. Fujita and H. Ohno, Biopolymers, 2010, 93, 1093-1099. Link

22. Biocompatible ionic liquids: a new approach for stabilizing proteins in liquid formulation, R. M. Vrikkis, K. J. Fraser, K. Fujita, D. R. MacFarlane, and G. D. Elliott, J. Biomec. Eng., 2009, 131, 0745141-0745144. Link

21. Polarized optical waveguide spectroscopy: Effective tool to analyze adsorption process of dye molecules, H. Ohno, K. Taniguchi, and K. Fujita, Optical Review, 2009, 16, 233-240. Link

20. Tetramethylammonium dihydrogen phosphate hemihydrate, K. Fujita, D. R. MacFarlane, K. Noguchi and H. Ohno, Acta Cryst., 2009, E65, o797. Link

19. Biocatalytic oxidation of cellobiose in an hydrated ionic liquid, K. Fujita, N. Nakamura, K. Igarashi, M. Samejima and H. Ohno, Green Chem., 2009, 11, 351-354. Link

18. Choline dihydrogen phosphate, K. Fujita, D. R. MacFarlane, K. Noguchi and H. Ohno, Acta Cryst., 2009, E65, o709. Link

17. Biocompatible ionic liquids: a new approach for stabilizing proteins in liquid formulation, R. Kemp, K. Fraser, K. Fujita, D. R. MacFarlane, and G. Elliott, Proceedings of the ASME 2008 Summer Bioengineering Conference SBC 2008

16. Solubility and stability of cytochrome c in hydrated ionic liquids: Effect of oxo acid residues and kosmotropicity, K. Fujita, D.R. MacFarlane, M. Forsyth, M. Yoshizawa-Fujita, K. Murata, N. Nakamura, and H. Ohno, Biomacromolecules, 2007, 8, 2080-2086. Link

15. A new class of proton-conducting ionic plastic crystals based on organic cations and dihydrogen phosphate, M. Yoshizawa-Fujita, K. Fujita, M. Forsyth, and D. R. MacFarlane, Electrochem. Commun., 2007, 9, 1202-1205. Link

14. Direct visible spectral analysis of solid samples by optical waveguide spectroscopy due to adsorbed sample molecules after sublimation, H. Takahashi, K. Fujita, and H. Ohno, Chem. Lett., 2007, 36, 116-117. Link

13. Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids, K. Fujita, M. Forsyth, D. R. MacFarlane, R. W Reid, and G. D Elliott, Biotechnology and Bioengineering, 2006, 94, 1209-1213. Link

12. Importance of poly(ethylene oxide)-modification and chloride anion for the electron transfer reaction of cytochrome c in 1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide, H. Ohno, C. Suzuki, and K. Fujita, Electrochim. Acta, 2006, 51, 3685-3691. Link

11. Protein solubilising and stabilising ionic liquids, K. Fujita, D. R. MacFarlane, and M. Forsyth, Chem. Commun., 2005, 4804-4806. Link

10. Dynamic analysis of aggregation of methylene blue with polarized optical waveguide spectroscopy, K. Fujita, K. Taniguchi, and H. Ohno, Talanta, 2005, 65, 1066-1070. Link

9. Mimicking protein-protein electron transfer: Voltammetry of pseudomonas aeruginosa azurin and the thermus thermophilus CuA domain at ω-derivatized self-assembled-monolayer gold electrodes, K. Fujita, N. Nakamura, H. Ohno, B.S. Leigh, K. Niki, H.B. Gray, and J.H. Richards, J. Am. Chem. Soc., 2004, 126, 13954-13961. Link

8. Oriented structure of octadecyl acridine orange intercalated in the monolayer and Langmuir-Blodgett film of octadecyl adenine-thymine base pairs, K. Fujita, F. Nakamura, and H. Ohno, Polym. Adv. Technol., 2004, 15, 567-572. Link

7. Coupling to Lysine-13 promotes electron tunneling through carboxylate-terminated alkanethiol self assembled monolayers to cytochrome c, K. Niki, W.R. Hardy, M.G. Hill, H. Li, J.R. Spinkle, E. Margoliash, K. Fujita, R. Tanimura, N. Nakamura, H. Ohno, J. H. Richards, and H.B. Gray, J. Phys. Chem., B, 2003, 107, 9947-9949. Link

6. Electron transfer process of poly(ethylene oxide) modified cytochrome c in imidazolium type ionic liquid, H. Ohno, C.Suzuki, K. Fukumoto, M. Yoshizawa, and K. Fujita, Chem. Lett., 2003, 32, 450-451. Link

5. Redox reaction of PEO-modified cytochrome c adsorbed on the electrode in ion conductive PEO matrix analyzed with non-contact optical waveguide spectroscopy, K. Fujita and H. Ohno, Polym. Adv. Technol., 2003, 14, 486-491. Link

4. Non-contact spectral analysis of cytochrome c on carbon electrodes with optical waveguide spectroscopy, K. Fujita, C. Suzuki, and H. Ohno, Electrochem. Commun., 2003, 5, 47-50. Link

3. Optical waveguide spectrometry of acridine orange in monolayer and Langmuir-Blodgett film, H. Ohno, S. Yoneyama, F. Nakamura, K. Fukuda, M. Hara, and M. Shimomura, Langmuir, 2002, 18, 1661-1665. Link

2. Electron transfer reaction of cytochrome c at the electrode surface analyzed with noncontact optical waveguide spectroscopy, K. Fukuda and H. Ohno, Electroanalysis, 2002, 14, 605-610. Link

1. Non-contact measurement of adsorbed cytochrome c with optical waveguide spectrometry; The effect of distance between waveguide and protein on the spectral sensitivity, H. Ohno, K. Fukuda, and F. Kurusu, Chem. Lett., 2000, 29, 76-77. Link


総説及び成書

Ionic liquids designed for advanced applications in bioelectrochemistry, K. Fujita, K. Murata, M. Masuda, N. Nakamura, and H. Ohno, RSC Advances, 2012, 2, 4018-4030. Link

タンパク質を未変性で溶解できるイオン液体の設計藤田恭子、 田村薫、 大野弘幸 ファインケミカル201140, 31-36.

Electrochemical Aspects of Ionic Liquids, 2nd Edition, H. Ohno ed., Wiley Interscience, New York, 2011, Chapter 7, 12, 13.

イオン液体III -ナノ・バイオサイエンスへの挑戦-, 監修:大野弘幸, シーエムシー出版2010, 第1, 2, 22, 23, 26章.

タンパク質の新溶媒としての水和イオン液体藤田恭子、大野弘幸, 高分子2009582, 88-88.

イオン液体II -驚異的な進歩と多彩な近未来-, 監修:大野弘幸, シーエムシー出版2006, 第14章, 154-156.

Electrochemical Aspects of Ionic Liquids, H. Ohno ed., Journal of Biomechanical Engineering2005131, Chapter 7, 12, 13.

測定法Q&A 電気化学 -そこを知りたい、議論したい -光導波路分光法による電気化学測定のノウハウ-, 大野弘幸、藤田恭子Electrochemistry200472, 521-523.

導波路分光法を用いた光不透過表面の解析, 大野弘幸、藤田恭子表面200341, 45-57.

界面の新しい解析法:非接触光導波路分光法, 大野弘幸、藤田恭子日本接着学会誌200238, 306-312.