Kouta Takeda
Position: Research Fellow of Biotechnology and Life Science
E-mail; kota.takeda23(at)gmail.com
Phone; +81(0)42 388 7467
Room; Builiding 12 #304
University; Tokyo University of Agriculture and Technology
Department; Biotechnology and Life Science
Address; Koganei, Tokyo 184-8588, Japan.
Educational Background
Mar. 2015, Doctor of Engineering, Dept. of Biotechnology and Life Science, Graduate School of TUAT
Doctor thesis: Study of a new eukaryotic pyrroloquinoline quinone dependent pyranose dehydrogenase
Mar. 2012, Master of Engineering, Dept. of Biotechnology and Life Science, Graduate School of Tokyo University of Agriculture and Technology
Master Thesis: A novel PQQ alcohol dehydrogenase as a catalyst for a bioanode
Mar. 2010, Bachelor of Engineering, Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology
Undergraduate thesis: Expression of recombinant quinoprotein alcohol dehydrogenase homologs
Work Experience
Research Fellowship for Young Scientists (PD): Apr. 2015-present
Research Fellowship for Young Scientists(DC2): Apr. 2014-Mar. 2015
Super Research Assistant: Feb. 2013-Mar. 2013
Research Assistant: Nov. 2012-Feb. 2013
Research
Characterization and application of novel PQQ dehydrogenases and some metalloproteins, such as cytochrome P450 and Fe-dependent Alcohol dehydrogenase, with focus on structure-activity relationship and electron transfer mechanism.
Research Subjects: Biochemistry / Electrochemistry
Techniques:
Protein expression, Electrochemical measurement, Protein crystallization, High-Speed Atomic Force Microscope
Keywords:
Pyrroloquinoline quinone / oxidoreductase / heme / Carbohydrate-Active Enzymes / Electron Transfer / Biosensor and Biofuel cell
collaborations:
Collaborating with Assoc. prof. Kiyohiko igarashi and Prof. Masahiro Samejima, Dept. of Biomaterial Sciences, Graduate School of Agriculture and Life Sciences, Tokyo Univ., Tokyo, Japan. To experiment in X-ray crystal structure analysis and extracellular oxidoreductases of cellulolytic fungi.
Assoc. prof. Takayuki Uchihashi, Dept. of Physics, Kanazawa Univ., Kanazawa, Japan. To optimize an electrochemical high-speed atomic force microscope.
Assoc. prof. Makoto Yoshida, Dept. of Environmental and Natural Resource Science, TUAT. To experiment in bioinformatics and gene analysis of cellulolytic fungi.
Publications
1. The two-step electrochemical oxidation of alcohols using a novel recombinant PQQ alcohol dehydrogenase as a catalyst for a bioanode, K. Takeda, H. Matsumura, T. Ishida, M. Samejima, K. Igarashi, N. Nakamura, and H. Ohno, Bioelectrochemistry, 2013, 94, 75-78. Link
2. Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase, K. Takeda,Takuya Ishida, Kiyohiko Igarashi, Masahiro Samejima, Nobuhumi Nakamura, Hiroyuki Ohno, Bioscience, Biotechnology, and Biochemistry, 2014, 78(7), 1195-1198.
3. Discovery of a eukaryotic pyrroloquinoline quinone-dependent oxidoreductase belonging to a new auxiliary activity family in the database of carbohydrate-active enzymes, Hirotoshi Matsumura*, Kiwamu Umezawa*, K. Takeda*, Naohisa Sugimoto, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, Nobuhumi Nakamura, PLoS ONE, 2014, 9(8), e104851.
4. Real-time dynamic adsorption processes of cytochrome c on an electrode observed through electrochemical high-speed atomic force microscopy, K. Takeda, Takayuki Uchihashi, Hiroki Watanabe, Takuya Ishida, Kiyohiko Igarashi, Nobuhumi Nakamura, Hiroyuki Ohno, PLoS ONE, 2015, 10(2), e0116685.
5. Characterization of a novel PQQ-dependent quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea classified into auxiliary activities family 12 in carbohydrate-active enzymes, K. Takeda*, Hirotoshi Matsumura*, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, Nobuhumi Nakamura, PLoS ONE, 2015, 10(2), e0115722.
6. A novel pyrroloquinoline quinone-dependent 2-keto-D-glucose dehydrogenase from?Pseudomonas aureofaciens, Kiwamu Umezawa, K. Takeda, Takuya Ishida, Naoki Sunagawa, Akiko Makabe, Kazuo Isobe, Keisuke Koba, Hiroyuki Ohno, Journal of bacteriology, 2015, 197(8), 1322-1329.
7. Multi-enzyme anode composed of FAD-dependent and NAD-dependent enzymes with a single ruthenium polymer mediator for biofuel cells, Riku Sakuta, K. Takeda, Takuya Ishida, Kiyohiko Igarashi, Masahiro Samejima, Nobuhumi Nakamura, Hiroyuki Ohno, Electrochemistry Communications, 2015, 56, 75-78.
8. pH-dependent electron transfer reaction and direct bioelectrocatalysis of the quinohemoprotein pyranose dehydrogenase from Coprinopsis cinerea, K. Takeda, Hirotoshi Matsumura, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, Nobuhumi Nakamura, Bioelectrochemistry, submitted.
*These authors contributed equally to this work.
Presentations
Oral presentation
1. ○Kota Takeda, Hirotoshi Matsumura, Kiyohiko Igarashi, Nobuhumi Nakamura, Masahiro Samejima, Hiroyuki Ohno, "A novel recombinant PQQ alcohol dehydrogenase as catalyst for bioanode: Two-step electrochemical oxidation of alcohols" Pacific Rim Meeting (PRiME 2012), Hawaii Convention Center and the Hilton Hawaiian Village, Honolulu, Hawaii, USA, October 7-12, 2012.
Additional 14 oral presentations in domestic conference.
Poster presentations
1. ○Kota Takeda, Hirotoshi Matsumura, Kiyohiko Igarashi, Nobuhumi Nakamura, Masahiro Samejima, Hiroyuki Ohno, " Expression and characterization of recombinant quinoprotein alcohol dehydrogenase homologs from Pseudomonas putida KT2440", The 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem2010), Honolulu, Hawaii, USA, December 15-20, 2010.
2. ○Kota Takeda, Misaki, Inukai, Hirotoshi Matsumura, Kiyohiko Igarashi, Nobuhumi Nakamura, Masahiro Samejima, and Hiroyuki Ohno, "Site directed mutagenesis within the heme domain of novel fucose dehydrogenase" The 6th Asian Biological Inorganic Chemistry Conference (AsBIC-VI), Hong Kong Polytechnic University, Hong Kong, China, November 5-8, 2012.
3. ○Kotua Takeda, Takuya Ishida, Hirotoshi Matsumura, Makoto Yoshida, Kiyohiko Igarashi, Masahiro Samejima, Nobuhumi Nakamura, and Hiroyuki Ohno, “Investigation of the pH-dependent electron transfer reaction of a novel sugar dehydrogenase from Coprinopsis cinerea”, International Conference on BioInorganic Chemistry (ICBIC 16), Grenoble, France, July, 2013.
4. ○Kouta Takeda, Takayuki Uchihashi, Hiroki Watanabe, Takuya Ishida, Kiyohiko Igarashi, Nobuhumi Nakamura, Hiroyuki Ohno, “Real-time imaging of dynamic adsorption processes of cytochrome c on the SAM modified gold electrode by electrochemical high-speed atomic force microscopy”, 8th International symposium on organic molecular electronics (ISOME 2014), Tokyo, Japan, May, 2014, Poster Award.
5.○Kouta Takeda, Hirotoshi Matsumura, Takuya Ishida, Masahiro Samejima, Hiroyuki Ohno, Makoto Yoshida, Kiyohiko Igarashi, Nobuhumi Nakamura, “Enzymatic properties of eukaryotic pyrroloquinoline quinone-dependent sugardehydrogenase belonging to a new auxiliary activity family 12”, 7th Asian Biological Inorganic Chemistry Conference (AsBIC7), Gold coast, Australia, Nov 30 - Dec 5, 2014.
Additional 14 oral presentations in domestic conference.
GRANTS
Apr. 2014-present
Grant-in-Aid?for Japan Society for the Promotion of Science (JSPS) Fellows (No. 14J08641)
AWARDS
Best Poster, "Electrochemical high-speed atomic force microscopy shows dynamic adsorption processes of a protein" Tokyo Univ. of Agri. and Technol. - The Univ. of Electro-Comm. Joint Symposium on Nano future material and Coherent photochemistry, December 15, 2012.
Best Poster, “Real-time imaging of dynamic adsorption processes of cytochrome c on the SAM modified gold electrode by electrochemical high-speed atomic force microscopy” The 8th International Symposium on Organic Molecular Electronics (ISOME 2014), May 15-16, 2014.
Affiliations
The Chemical Society of Japan.
The Society for Biotechnology, Japan.
The Japanese Biochemical Society
