The delivery of biomolecules in cells currently depend greatly on conventional methods such as electroporation, conjugation and transduction. However, although highly applicable, these methods can be tedious and time consuming. Low transformation efficiency and their applicability to certain bacterial strains is also known as a limitation. In our group, we try to employ cell-penetrating peptides as a tool to overcome some of these highlighted challenges.


PUBLICATION:  Inoue G. et al., ACS Appl. Bio Mater. 4:3462  (2021)

COLLABORATORS: Muraoka Lab, Tokyo University of Agriculture and Technology
PRESS RELEASE: 高い効率で細菌の細胞内へ透過するペプチドを開発-多種の細菌へ簡便に薬剤を導入し、細菌操作や殺菌を可能にする新物質-
SUMMARY: To further promote the efficicency of cell-penetrating peptide (CPP) permeation in diverse bacterial species, the critical side chain effects of CPP was evaluated. As a result, the introduction of shorter side chains to CPP sequences elevated CPP permeation in bacteria. 
多様な細菌種における細胞透過性ペプチド (CPP)の透過効率をさらに向上するために、CPP の重要な側鎖効果を評価した。その結果、より短い側鎖をCPP配列に挿入すると、細菌におけるCPP透過効率が上昇した。

PUBLICATION: Toyohara D et al., Front Microbiol. 10:2534 (2019)

COLLABORATORS: Muraoka Lab, Tokyo University of Agriculture and Technology
SUMMARY: To evaluate the applicability of cell-penetrating peptides (CPPs) as a biomolecule carrier with diverse bacterial strains, we evaluated and optimized factors that may play an important role in elevating CPP permeation efficiency. Based on our evaluation, we showed that 2 abiotic factors, temperature and tonicity promoted CPP permeation. Using our optimized conditions, we showed that CPP permeation was promoted in 9 out of 10 Enterobacteriaceae strains.