Abstract

Induced resistance and accessibility in rice plant using the incompatible and the compatible races of rice blast fungus: Some aspects of induced resistance and accessibility at microscopic level were investigated using the transformants expressing enhanced green fluorescence protein (EGFP) and red fluorescence protein (DsRed2). Pre-inoculation with the incompatible race on rice leaf sheath significantly suppressed invasive growth of the challenging compatible race. Strong auto-fluorescence on cell wall and HR were often observed in the cells infected with the compatible race, implying that resistance reaction was induced. But the induced areas were limited around ca. 500μm from the sites pre-infected with the incompatible race, suggesting that the resistance is induced locally rather then systemically. On the other hand, Pre-inoculation with the compatible race significantly suppressed resistance in the attacked cells and allowed the incompatible race to infect the cell. However the accessibility was limited only in the attacked or adjacent cells.