Abstract

Cong:1-2 is a virulence-loss-mutant of the soilborne cabbage yellows fungus ( Fusarium oxysporum f. sp. conglutinans; isolate Cong:1-1) emerged during repeated passages. Pre-treatment of cabbage seedlings with Cong:1-2 showed reduction of yellows, suggesting that Cong:1-2 carried biocontrol activity.1, 2) 2D electrophoresis showed a protein ( pI ~5.2, ~75 kD ) highly expressed especially in Cong:1-2. This protein was presumed as a GMC oxidoreductase by BLAST analysis. The gene encoding this protein in Cong:1-2 was cloned, and the putative ORF ( 1974 bp ) composed of two exons was named odx1. The ~5-kb genomic sequences including odx1 showed 100% homology between Cong:1-1 and Cong:1-2. To examine the relationships between ODX1 and pathogenicity or biocontrol activity, we generated odx1-disruptants from Cong:1-2.
1) Yoshida et al, Ann. Phytopathol. Soc. Jpn. 64, 611 (1998); 2) Nomura et al, Ann. Phytopathol. Soc. Jpn. 64, 612 (1998)

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